We narrowed to 16,161 results for: nans

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pNIR-Fb21

Plasmid

#184679

Purpose

NIR-Fb to spike-Cov-19 RBD

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb21 (S Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-zfCSF3b

Plasmid

#168202

Purpose

Express zebrafish Csf3b, mRNA synthesis

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

Insert

Csf3b (csf3b Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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PdnaK-IR3 GFP pZa

Plasmid

#170084

Purpose

GFP expression under the control of E. coli dnaK promoter engineered with IR3 HAIR motif from M. tuberculosis

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PdnaK-IR3

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Flag-stm2585

Plasmid

#174397

Purpose

Native S. Typhimurium gene stm2585 (sarA/steE) with N-terminal GFP and Flag tags.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Tags

EGFP and Flag

Expression

Mammalian

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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PdnaK-IR2 GFP pZa

Plasmid

#170081

Purpose

GFP expression under the control of E. coli dnaK promoter engineered with IR2 HAIR motif from M. tuberculosis

Depositor

Urartu Seker

Article

Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

Insert

Green fluorescent protein

Use

Synthetic Biology

Expression

Bacterial

Promoter

PdnaK-IR2

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK129-stm2585_S130A

Plasmid

#174403

Purpose

Bacterial expression of sarA with its GSK3-binding SxxxSP motif mutated to SxxxAP.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Expression

Bacterial

Mutation

sarA with S130A mutation

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pWSK129-stm2585_Y167F

Plasmid

#174402

Purpose

Bacterial expression of sarA with its STAT3-binding YxxQ motif mutated to FxxQ

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Expression

Bacterial

Mutation

sarA with Y167F mutation

Promoter

Endogenous

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-gp130dimer-stm2585

Plasmid

#174395

Purpose

Constitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

IL6ST cytosolic domain and sarA chimera

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-stm2585

Plasmid

#174391

Purpose

S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with N-terminal Flag tag

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

stm2585

Tags

Flag

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-zfCSF3a

Plasmid

#168201

Purpose

Express zebrafish Csf3a, mRNA synthesis

Depositor

Petr Bartunek

Article

Stachura et al Blood. 2013 Dec 5;122(24):3918-28. doi:

Insert

Csf3a (csf3a Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Mutation

Deletion of A27

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-Six1

Plasmid

#158532

Purpose

Expresses mouse Six1 in mammalian cells

Depositor

Neil Segil

Article

Menendez et al Elife. 2020 Jun 30;9. pii: 55249. doi: 1

Insert

SIX Homeobox 1 (Six1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP–acVHH–PB

Plasmid

#169685

Purpose

An anti-caffeine nanobody responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.

Depositor

Yubin Zhou

Article

Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214

Insert

anti-caffeine nanobody

Tags

modified STIM1-PB tag

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLV.U6gLacZ.EFS-NS.H2B-RFP

Plasmid

#170363

Purpose

Negative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B-RFP

Use

Lentiviral

Promoter

EFS-NS

Available Since

June 27, 2021

Availability

Academic Institutions and Nonprofits only

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Ash1

Plasmid

#169711

Purpose

Bacterial Expression of yeast Ash1 truncation (encodes amino acids 420-500)

Depositor

Tanja Mittag

Article

Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323

Insert

Ash1 (ASH1 Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

Truncation including encoding amino acids 420-500

Promoter

T7

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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GLN, Linear-3, PolyU(1), Hairpin Distance = 0nt

Plasmid

#166986

Purpose

Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(1), Hairpin = 0 nt

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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GLY, Linear-3, PolyU(4), Hairpin Distance = 0nt

Plasmid

#166991

Purpose

Tests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(4), Hairpin = 0 nt

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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GLY, Linear-3, PolyU(1), Hairpin Distance = 0nt

Plasmid

#166990

Purpose

Tests for the impact of 1 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLY tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(1), Hairpin = 0 nt

Expression

Mammalian

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-GCaMP6f-4x128-mAGNET

Plasmid

#166023

Purpose

Expresses GCaMP6f in CaMKII+ cells with low miRNA-128 expression

Depositor

Xue Han

Article

Keaveney et al J Neurosci. 2020 Dec 9;40(50):9576-9588.

Insert

GCaMP6f with 4 miR-128 binding sites

Use

AAV and Mouse Targeting

Tags

None

Expression

Mammalian

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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GLN, Linear-3, PolyU(4), Hairpin Distance = 0nt

Plasmid

#166987

Purpose

Tests for the impact of 4 uracil in conjunction with a hairpin structure in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(4), Hairpin = 0 nt

Expression

Mammalian

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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Donor-PIGAshort

Plasmid

#153547

Purpose

Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

PIGA (a 603-bp fragment from intron 5 and exon 6)

Use

Donor plasmid

Tags

N/A

Mutation

No mutation

Promoter

No promoter

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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