We narrowed to 14,583 results for: pET

1. pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)

   Plasmid

   #61938

   Purpose

   Co-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Inserts

   hUCH37 (ISF2) (UCHL5 Human)
   hNFRKB (39-156) (NFRKB Human)
   

   Use

   Tags

   6x HIS

   Expression

   Bacterial

   Mutation

   Construct ends at residue 156, residues 2-38 dele…

   Promoter

   T7

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

2. HOXC6 (human) HIS-tag pET

   Plasmid

   #8565

   Depositor

   Corey Largman

   Insert

   HOXC6 (HOXC6 Human)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   This protein starts at an internal Met initially …

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pET30a-ASXL1-HARE-HTH domain

   Plasmid

   #107164

   Purpose

   Expresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. coli

   Depositor

   Douglas Feldman

   Article

   Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

   Insert

   ASXL1 (Asxl1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Contains amino acids 2-83

   Promoter

   T7

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-FgßF3-ddFLN4-HIS-ybbr

   Plasmid

   #101743

   Purpose

   mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

   Depositor

   Hermann Gaub

   Article

   Milles et al Science. 2018 Mar 30;359(6383):1527-1533

   Insert

   FgßF3 peptide, ddFLN4 fingerprint (FGB Dictyostelium discoideum, Human)
   

   Use

   Tags

   HIS and ybbr

   Expression

   Bacterial

   Mutation

   C18S in ddFLN4, added 9F in FgßF3

   Promoter

   T7

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. delNC C-His Ana GvpC in pET28a

   Plasmid

   #85736

   Purpose

   Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

   Insert

   Gas vesicle protein C (truncated and his-tagged)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Deletion of N and C terminal regions, addition of…

   Promoter

   T7

   Available since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET51b-His-TEV-SNAP-tag_cx

   Plasmid

   #167273

   Purpose

   Overexpression of crystallizing SNAP-tag variant (P179R) in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   SNAP-tag
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   P179R

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET151-GFP-PP-RPN2 (925-953)

   Plasmid

   #73744

   Purpose

   Expression of GFP-PP-RPN2 (925-953) fusion in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN2 (RPN2 Human)
   

   Use

   Tags

   6x His and GFP

   Expression

   Bacterial

   Mutation

   Construct possesses only residues 932-953 of RPN2

   Promoter

   T7

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)

   Plasmid

   #117910

   Purpose

   Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uM

   Depositor

   Kai Johnsson

   Article

   Yu et al Science. 2018 Sep 14;361(6407):1122-1126

   Insert

   SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
   

   Use

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Mutation

   R44A on eDHFR

   Promoter

   T7

   Available since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b/GgVcl 884-1066 (aka Vt)

   Plasmid

   #46176

   Depositor

   Susan Craig

   Article

   Steimle et al J Biol Chem. 1999 Jun 25;274(26):18414-2

   Insert

   Vcl 884-1066 (VCL Chicken)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   aa 884-1066 (tail domain)

   Promoter

   T7

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-ybbR-HIS-CBM-CohI

    Plasmid

    #58709

    Purpose

    Probing molecule to perform single molecule force spectroscopy experiments

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    CBM-CohI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    changed Alanine 2 to Cysteine in CBM domain

    Promoter

    T7

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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