We narrowed to 7,572 results for: Lif

1. pMAL-MBP-KRas-Q61L

   Plasmid

   #214300

   Purpose

   Express constitutively active KRas fused with MBP

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Insert

   Constitutively active KRas (KRAS Human)
   

   Use

   Tags

   Maltose-binding protein (MBP)

   Expression

   Bacterial

   Mutation

   KRas-Q61L

   Promoter

   Tac

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMAL-MBP-KRas-S17N

   Plasmid

   #214301

   Purpose

   Express dominant negative KRas fused with MBP

   Depositor

   Cliff Stains

   Article

   He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

   Insert

   Dominant negative KRas (KRAS Human)
   

   Use

   Tags

   Maltose-binding protein (MBP)

   Expression

   Bacterial

   Mutation

   KRas-S17N

   Promoter

   Tac

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-Creb5(T59/T61A)(HA)

   Plasmid

   #195025

   Purpose

   Gateway vector containing HA-tagged Creb5 with T59/T61A mutation

   Depositor

   Andrew Lassar, Cheng-Hai Zhang

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   HA-tagged Creb5 (full length, 508aa; with with T59/T61A mutation) (CREB5 Bovine)
   

   Use

   Gateway cloning

   Tags

   3xHA

   Expression

   Mutation

   T59/T61A

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-Creb5(T59/T61D)(HA)

   Plasmid

   #195026

   Purpose

   Gateway vector containing HA-tagged Creb5 with T59/T61D mutation

   Depositor

   Andrew Lassar, Cheng-Hai Zhang

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   HA-tagged Creb5 (full length, 508aa; with with T59/T61D mutation) (CREB5 Bovine)
   

   Use

   Gateway cloning

   Tags

   3xHA

   Expression

   Mutation

   T59/T61D

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR

   Plasmid

   #207136

   Purpose

   DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

   Insert

   5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP Cav2.2 D122A pcDNA3

   Plasmid

   #206068

   Purpose

   expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a D122A mutation that disrupts the interaction with ?2?-1

   Depositor

   Annette Dolphin

   Article

   Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cav2.2 BBS W391A pcDNA3

   Plasmid

   #206077

   Purpose

   Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop that prevents Beta binding

   Depositor

   Annette Dolphin

   Article

   Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   W391A

   Promoter

   CMV

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. cacna2d1 HA D491A pcDNA3

   Plasmid

   #206070

   Purpose

   expression of rat calcium channel alpha2delta-1 with a single HA tag inserted after aa549 and a D491A mutation that prevents Gabapentin binding

   Depositor

   Annette Dolphin

   Article

   Gumerov et al Proc Natl Acad Sci U S A. 2022 Mar 8;119

   Insert

   cacna2d1 (Cacna2d1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D491A

   Promoter

   CMV

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. hPCNA-C148S

   Plasmid

   #190939

   Purpose

   untagged human PCNA with a cysteine to serine mutation

   Depositor

   Brian Kelch

   Article

   Magrino et al J Biol Chem. 2023 May;299(5):104656. doi

   Insert

   Proliferating Cell Nuclear Antigen (PCNA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed cysteine 148 to serine

   Promoter

   T7

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSUMO His6 SUMO SnRK2.3 (19-361)

    Plasmid

    #177846

    Purpose

    Bacterial Expression of SnRK2.3

    Depositor

    Karsten Melcher

    Article

    Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

    Insert

    SNF1-related protein kinase 2.3 (SNRK2.3 Mustard Weed)
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AdMKP-3 S159AS197A

    Plasmid

    #131523

    Purpose

    Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells

    Depositor

    Haiyan Xu

    Article

    Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

    Insert

    Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
    

    Use

    Adenoviral

    Tags

    No

    Expression

    Mutation

    changed Serine 159 to Alanine and Serine 197 to A…

    Promoter

    CMV

    Available since

    Oct. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST47-ARL4D

    Plasmid

    #67468

    Purpose

    Mammalian expression of hArl4D with stop codon (native expression)

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL4D (ARL4D Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    bp 272 A to C; N91T mutation; bp 327 T to G; sil…

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pDEST40-ARL4D-V5-His6

    Plasmid

    #67447

    Purpose

    Mammalian expression of hArl4D with C-term V5 and His6 tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL4D (ARL4D Human)
    

    Use

    Tags

    His and V5

    Expression

    Mammalian

    Mutation

    bp 272 A to C; N91T mutation; bp 327 T to G; sil…

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pET-DEST42-ARL4D-V5-His6

    Plasmid

    #67426

    Purpose

    Expression of hArl4D with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL4D (ARL4D Human)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial

    Mutation

    bp 272 A to C; N91T mutation; bp 327 T to G; sil…

    Promoter

    T7/lacO

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pDEST47-ARL4D-GFP

    Plasmid

    #67405

    Purpose

    Mammalian expression of hArl4D with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL4D (ARL4D Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    bp 272 A to C; N91T mutation; bp 327 T to G; sil…

    Promoter

    CMV

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pDONR221-ARL4D-no stop

    Plasmid

    #67337

    Purpose

    hArl4D without stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL4D (ARL4D Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Bacterial

    Mutation

    bp 272 A to C; N91T mutation; bp 327 T to G; sil…

    Promoter

    None

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pDONR221-ARF4-no stop

    Plasmid

    #67311

    Purpose

    hArf4 without stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF4 (ARF4 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Bacterial

    Mutation

    V53A, L123P and M134I mutations were unintended P…

    Promoter

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pCR2.1-Ins2-842

    Plasmid

    #53969

    Purpose

    842 bp insert from mouse Ins2 gene used as a control for methylation-specific PCR assays

    Depositor

    Kevin Ferreri

    Article

    Husseiny et al PLoS One. 2012;7(10):e47942. doi: 10.137

    Insert

    mouse Ins2 gene partial (Ins2 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1 Flag YFP hNKCC1 P676C I730C (NT809)

    Plasmid

    #50866

    Purpose

    Expresses human NKCC1 P676C I730C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    P676C I730C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1 Flag YFP hNKCC1 I674C (NT475)

    Plasmid

    #50875

    Purpose

    Expresses human NKCC1 I674C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    I674C in hNKCC1 in NT17

    Promoter

    CMV

    Available since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge