We narrowed to 18,937 results for: Tec

1. mKO-Fibrillarin-7

   Plasmid

   #57842

   Purpose

   Localization: Nuclear - Nucleolus, Excitation: 548, Emission: 561

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Fibrillarin (FBL Human)
   

   Tags

   mKO

   Expression

   Mammalian

   Mutation

   R36Q, S66F

   Promoter

   CMV

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMDC32-FIT2

   Plasmid

   #96990

   Purpose

   Express mouse FIT2 gene in plants.

   Depositor

   Kent Chapman

   Article

   Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

   Insert

   MmFIT2 (Fitm2 Mouse)
   

   Expression

   Plant

   Promoter

   35S

   Available Since

   Aug. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCTCON2-BXG-2TAG

   Plasmid

   #158138

   Purpose

   Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (2 TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   BFP-GFP fragment
   

   Expression

   Yeast

   Mutation

   Cloned in a TAG codon at the first serine residue…

   Promoter

   GAL1-10

   Available Since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)

   Plasmid

   #160635

   Purpose

   TU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminator

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2022 Feb;20(2):399-4

   Insert

   AcrIIA4
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   35S

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-dLbCpf1-BE0ΔiNLS

   Plasmid

   #107684

   Purpose

   Expresses pCMV-dLbCpf1-BE0ΔiNLS crRNA in mammalian cells

   Depositor

   Jia Chen

   Article

   Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   dLbCpf1-BE0ΔiNLS
   

   Expression

   Mammalian

   Mutation

   D832A/E925A/D1148A

   Promoter

   CMV

   Available Since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUPD2 AtU6-26:LbCas12a_DR (GB1443)

   Plasmid

   #160575

   Purpose

   LbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   AtU6-26:LbCas12a_DR
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   U6-26

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCfB3038(XII-1 MarkerFree)

   Plasmid

   #73278

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-1 (Chr XII: 795787..796720)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCfB3040(XII-4 MarkerFree)

   Plasmid

   #73280

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-4 (Chr XII:830227..831248)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPK1E-1-gfp

   Plasmid

   #48133

   Purpose

   Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

   Depositor

   Dieter Jahn

   Article

   Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

   Insert

   gfp
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. TAL2317

    Plasmid

    #36743

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FGFR1-TALEN_Right (FGFR1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

11. TAL2316

    Plasmid

    #36742

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    FGFR1-TALEN_Left (FGFR1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Promoter

    CMV

    Available Since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pCEP4-HA-Flag-natT1R2 ECD

    Plasmid

    #113954

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 extracellular domain with signal peptide of influenza hemagglutinin

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Tags

    FLAG and Signal/leader sequence from influenza he…

    Expression

    Mammalian

    Mutation

    residues 22-568

    Promoter

    cmv

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBW1997_pExpr-hU6-CRISPR-DECODER

    Plasmid

    #87549

    Purpose

    Expresses NGN2, MIAT, ACTC1, and TTN gRNAs in response to combinations of Cre and Flp recombinases

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    gRNAs targeting NGN2, MIAT, ACTC1, TTN
    

    Use

    CRISPR, Cre/Lox, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCellFree_G03 GTF2A1

    Plasmid

    #67076

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2A1 (GTF2A1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pCellFree_G03 BTF3

    Plasmid

    #67059

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BTF3 (BTF3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCellFree_G03 SMAD2

    Plasmid

    #67095

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    SMAD2 (SMAD2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pLenti-LmoCascade-IL1RNcrRNA

    Plasmid

    #126493

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting IL1RN

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    Lmo IL1RN cr03
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-LmoCascade-HBE1crRNA

    Plasmid

    #126494

    Purpose

    Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting HBE1

    Depositor

    Charles Gersbach

    Article

    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

    Insert

    Lmo HBE1 cr06
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCellFree_G03 TYK2

    Plasmid

    #67133

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TYK2 (TYK2 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Mutation

    A435S

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. Zebrafish-gRNA-0002

    Plasmid

    #42242

    Purpose

    gRNA targeted to zebrafish gene drd3

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Insert

    gRNA-drd3 (drd3 Zebrafish)
    

    Use

    CRISPR; Zebrafish expression

    Promoter

    T7

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only