We narrowed to 16,131 results for: form

1. Human HMG-CoA lyase (HL)

   Plasmid

   #52433

   Purpose

   bacterial expression of human HMG-CoA lyase (HL)

   Depositor

   Henry Miziorko

   Article

   Montgomery et al Arch Biochem Biophys. 2011 Jul;511(1-2):

   Insert

   HMG CoA lyase (HMGCL Human)
   

   Tags

   His and S

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 8, 2014

   Availability

   Academic Institutions and Nonprofits only

2. GFP-C-CARMIL2b

   Plasmid

   #118741

   Purpose

   To express CARMIL2 with GFP tag on N-terminus

   Depositor

   John Cooper

   Article

   Lanier et al Mol Biol Cell. 2015 Dec 15;26(25):4577-8

   Insert

   CARMIL2 (CARMIL2 Human)
   

   Tags

   AcGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiGuide-Neo-CTRLg2

   Plasmid

   #139451

   Purpose

   Lentiviral vector with non-targeting gRNA and neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   Non-targeting sgRNA inserted; resistance gene: neoR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Neo1.a-AP-His

   Plasmid

   #71963

   Purpose

   Expresses the extracellular region of the Neogenin 1, isoform a protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Neo1.a (Neo1 Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. TOPO tTR-KRAB-rtTA3-Luc

   Plasmid

   #68449

   Purpose

   TOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible gene

   Depositor

   Tyler Jacks

   Article

   Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

   Insert

   tTR-KRAB-2A-rtTA3-2A-Luciferase
   

   Use

   Luciferase; Gmap

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5/mCherry-pBirA-HA

   Plasmid

   #154458

   Purpose

   expresses mCherry-pBirA-HA/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   mCherry-pBirA-HA
   

   Expression

   Mammalian

   Promoter

   CMV-F, T7 promoter

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBeast-pAhpC-RBS- LacZ

   Plasmid

   #190060

   Purpose

   LacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoter

   Depositor

   Jerome Bonnet

   Article

   Soudier et al ACS Synth Biol. 2022 Aug 19;11(8):2578-2

   Insert

   lacZ
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-hPPP3R1

   Plasmid

   #179136

   Purpose

   Expression vector of human protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1), CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

   Insert

   protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. FgH1tUTG_huBim_Ex3

   Plasmid

   #85532

   Purpose

   Inducible expression of guide RNA (huBim_Ex3) with fluorescent GFP reporter

   Depositor

   Marco Herold

   Article

   Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

   Insert

   hu Bim
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   H1t

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. LentiGuide-Puro-CTRLg1 (CG509)

    Plasmid

    #139455

    Purpose

    Lentiviral vector with non-targeting gRNA and puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    Non-targeting sgRNA inserted; resistance gene: puroR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCSD_2XNLS-SpCas9WT-NLS-3xHA-2XNLS-NmCas9WT-2XNLS

    Plasmid

    #107324

    Purpose

    Expresses SpCas9 fused to NmCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-NmCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 is fused to NmCas9

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-Q769gp120-8his

    Plasmid

    #100929

    Purpose

    Mammalian expression plasmid for gp120 subunit from the Q769.d22 HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (Q769.d22) Env
    

    Tags

    CD5 leader sequence and Purification tag after K5…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRetro-XT-PAK2-CFP(puro)-T402A

    Plasmid

    #81179

    Purpose

    Retroviral vector for expression of kinase inactive PAK2 with a CFP fusion

    Depositor

    Angeliki Malliri

    Article

    Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

    Insert

    p21 (RAC1) activated kinase 2 (PAK2 Human)
    

    Use

    Retroviral

    Tags

    CFP

    Mutation

    T402A

    Promoter

    P-tight TRE

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLIK-Neo-TGmiR-Luc

    Plasmid

    #25745

    Purpose

    Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA and GFP, constitutive Neomycin resistance gene coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    Luciferase miR-shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223 GABARAPL2 WT

    Plasmid

    #123086

    Purpose

    Gateway entry clone encoding human GABARAPL2 wild-type

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
    

    Use

    Gateway entry vector / entry clone

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCI-neo-mApg5

    Plasmid

    #22956

    Depositor

    Noboru Mizushima

    Article

    Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

    Insert

    autophagy related genes 5 (Atg5 Mouse)
    

    Expression

    Mammalian

    Available Since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pBabe puro H-Ras G12V E37G

    Plasmid

    #12275

    Depositor

    Bob Weinberg

    Article

    Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

    Insert

    H-Ras G12V E37G (HRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Contains G12V activating mutation. The E37G mutat…

    Available Since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

18. KM-CashGem-Xyl2-URA3-LINEAR

    Plasmid

    #174838

    Purpose

    pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Promoter

    TEF1p

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. attB-­GFP-­Poly(A)‐NeoR

    Plasmid

    #65526

    Purpose

    NeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-GFP
    

    Use

    Mouse Targeting; Bxb1

    Expression

    Mammalian

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cav2.2e[Δa10, Δ18a, 24a, Δ31a, 37b, 46]

    Plasmid

    #26571

    Depositor

    Diane Lipscombe

    Article

    Lin et al Neuron. 1997 Jan . 18(1):153-66.

    Insert

    calcium channel alpha-1B subunit (Cacna1b Rat)
    

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Deletion of exons a10, 18a, and 31a Contains e…

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only