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pGEX-5X-1-MePCE_aa470-689

Plasmid

#113544

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 470 - 689.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1_MePCE_aa432-689

Plasmid

#113543

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 432 - 689.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1_MePCE_aa400-689

Plasmid

#113542

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 400 - 689.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1_MePCE_aa1-400

Plasmid

#113541

Purpose

Produces an N-terminal GST-tagged MePCE protein consisting of amino acids 1 - 400.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Sesn-C mSesn2 (delAB)

Plasmid

#111799

Purpose

Mammalian Expression of mSesn2

Depositor

Jun Hee Lee

Article

Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

Insert

mSesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

AB domain truncation

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Sesn-B+C mSesn2 (delA)

Plasmid

#111798

Purpose

Mammalian Expression of mSesn2

Depositor

Jun Hee Lee

Article

Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

Insert

mSesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

A domain truncation

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Sesn-A mSesn2 (delBC)

Plasmid

#111797

Purpose

Mammalian Expression of mSesn2

Depositor

Jun Hee Lee

Article

Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

Insert

mSesn2 (Sesn2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

BC domain truncation

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-BirA-UBE3B-Puro

Plasmid

#107351

Purpose

Expresses UBE3B fused to BirA in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

BirA-UBE3B (UBE3B Human)

Use

Lentiviral

Tags

Biotin ligase

Expression

Mammalian

Promoter

CMV

Available Since

June 7, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCR3-vsv-CenpB 1-166

Plasmid

#108474

Purpose

expression of vsv-CenpB 1-166

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

CenpB 1-166 AA (CENPB Human)

Tags

VSV

Expression

Mammalian

Mutation

encodes only the DNA-binding domain of CENP-B

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-Mis12-dCEN-Incenp

Plasmid

#108477

Purpose

expression of vsv-Mis12-dCEN-INCENP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12-dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

CEN box replaced by Mis12

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET15b+C3(R112C/T180C)X

Plasmid

#108196

Purpose

Expresses P450cam-PCNA3(R112C/T180C)-PdX fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

Insert

P450cam-PCNA3(R112C/T180C)-PdX fusion protein

Tags

His6 tag

Expression

Bacterial

Mutation

R112C/T180C(PCNA3); C73S/C85S (PdX)

Promoter

T7 promoter

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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Twitch-2C pRSETB

Plasmid

#104500

Purpose

fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Article

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Insert

Twitch-2C

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only
pCDNA3-SPA mimic WT

Plasmid

#92350

Purpose

To mimic SPA RNA processing and expression

Depositor

Ling-Ling Chen

Article

Wu et al Mol Cell. 2016 Nov 3;64(3):534-548. doi:

Insert

SNRPN exon 9, intron9, exon10, intron10 partial partial of SPA1 (SNRPN Human)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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p35S::SISOBIR1-YFPc

Plasmid

#86164

Purpose

SOBIR1 split YFP

Depositor

Silke Robatzek

Article

Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

Insert

SOBIR1 (SOBIR1 Mustard Weed)

Tags

YFPc

Expression

Plant

Promoter

35S

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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LLhT-pHG165a V52A

Plasmid

#90050

Purpose

Constitutive expression of a chimeric LacI:TreR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhT/V52A

Expression

Bacterial

Mutation

V52A

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhA-pHG165a Q55A

Plasmid

#90052

Purpose

Constitutive expression of a chimeric LacI:AscG transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhA/Q55A

Expression

Bacterial

Mutation

Q55A

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LLhC-pHG165a Q55I

Plasmid

#90053

Purpose

Constitutive expression of a chimeric LacI:CytR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

Insert

LLhC/Q55I

Expression

Bacterial

Mutation

Q55I

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-) + SLNCR1 _2095-2257

Plasmid

#86825

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-) + SLNCR1 _884-1285

Plasmid

#86822

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-) + SLNCR1 _1-480

Plasmid

#86817

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-) + SLNCR1 372-672 (conserved sequence)

Plasmid

#86826

Purpose

Expression of conserved region of lncRNA SLNCR1

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
p35S::AtSOBIR1-YFPn

Plasmid

#86165

Purpose

SOBIR1 split YFP

Depositor

Silke Robatzek

Article

Postma et al New Phytol. 2016 Apr;210(2):627-42. doi:

Insert

SOBIR1 (SOBIR1 Mustard Weed)

Tags

YFPn

Expression

Plant

Promoter

35S

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-a4-delta tail

Plasmid

#80018

Purpose

Mammalian expression of human integrin alpha4 with no tail

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

integrin alpha4 (ITGA4 Human)

Expression

Mammalian

Mutation

no cytoplasmic tail (amino acids 1 to 1007)

Promoter

CMV

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pPB-CAG-Mbd3b-pA-pgk-bsd

Plasmid

#74920

Purpose

Mammalian expression of Mbd3b

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Insert

Mnd3b (Mbd3 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only
pPB-CAG-Mbd3c-pA-pgk-bsd

Plasmid

#74921

Purpose

Mammalian expression of Mbd3c

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Insert

Mbd3c (Mbd3 Mouse)

Expression

Mammalian

Promoter

CAG

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Ywhab-3'UTR-MRE-1 MUT

Plasmid

#61796

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhab with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhab 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Xbp1-3'UTR-MRE-3 MUT

Plasmid

#61793

Purpose

Luciferase reporter containing the 3'UTR of mouse Xbp1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Xbp1 3'UTR with miR-200c MRE-3 mutated

Use

Luciferase

Mutation

miR-200c MRE-3 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Smad5-3'UTR-MRE-1 MUT

Plasmid

#61791

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad5 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad5 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Map3k1-3'UTR-MRE-1 MUT

Plasmid

#61790

Purpose

Luciferase reporter containing the 3'UTR of mouse Map3k1 with MRE-1 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Map3k1 3'UTR with miR-200c MRE-1 mutated

Use

Luciferase

Mutation

miR-200c MRE-1 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT

Plasmid

#61789

Purpose

Luciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutated

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated

Use

Luciferase

Mutation

both miR-200c MRE-2 and MRE-3 mutated

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only