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Cas9_P2A_POLB

Plasmid

#190145

Purpose

Expresses SpCas9 and POLB

Depositor

Timothy Lu

Article

Nakade et al bioRxiv 2022.12.05.518807

Insert

SpCas9_P2A_POLB

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Codon Optimized for mammalian cells

Promoter

CBh

Available Since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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loxP-T2A-Myr-Casp8-ERT2-rox

Plasmid

#182362

Purpose

Shuttle vector for T2A-Myr-Casp8-ERT2

Depositor

Mario Capecchi

Insert

loxP-T2A-Myr-Casp8-ERT2-rox

Use

Cre/Lox and Mouse Targeting; Dre / rox

Expression

Mammalian

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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loxP-T2A-Myr-DeadCasp8-ERT2-rox

Plasmid

#182361

Purpose

Shuttle vector for T2A-Myr-DeadCasp8-ERT2

Depositor

Mario Capecchi

Insert

loxP-T2A-Myr-DeadCasp8-ERT2-rox

Use

Cre/Lox and Mouse Targeting; Dre / rox

Expression

Mammalian

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAM-35s-Snrk2.3-YFPn-1

Plasmid

#102391

Purpose

split YFP. Plant expression of Snrk2.3-YFPn-1

Depositor

Silke Robatzek

Insert

SNRK2.3 (SNRK2.3 Mustard Weed)

Tags

YFPn

Expression

Plant

Promoter

35S

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCM305

Plasmid

#44550

Depositor

Michael Grunstein

Article

Millar et al Genes Dev. 2006 Mar 15;20(6):711-22.

Insert

HA-htz1

Expression

Yeast

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
pPR244-Bic-C

Plasmid

#26139

Depositor

Phillip Newmark

Article

Wang et al Genes Dev. 2010 Sep 15. 24(18):2081-92.

Insert

Smed-Bicaudal-C

Use

RNAi

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pPR244-NF-YB

Plasmid

#26147

Depositor

Phillip Newmark

Article

Wang et al Genes Dev. 2010 Sep 15. 24(18):2081-92.

Insert

Smed-NF-YB

Use

RNAi

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only
pBS-NF-YB

Plasmid

#26136

Depositor

Phillip Newmark

Article

Wang et al Genes Dev. 2010 Sep 15. 24(18):2081-92.

Insert

Smed-NF-YB

Use

Cloning

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only
pYBS139

Plasmid

#18900

Depositor

Elaine Elion

Article

Kranz et al Genes Dev. 1994 Feb 1. 8(3):313-27.

Insert

FAR1 (FAR1 Budding Yeast)

Expression

Yeast

Available Since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut A

Plasmid

#11314

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation A (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut B

Plasmid

#11315

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation B (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut C

Plasmid

#11316

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation C (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut D

Plasmid

#11317

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation D (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut E

Plasmid

#11318

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation E (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut F

Plasmid

#11319

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation F (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut H

Plasmid

#11321

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation H (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut GFP

Plasmid

#11323

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 bulged binding sites for CXCR4 siRNA antisense, flanking 2 binding sites for GFP

Use

Luciferase

Tags

Rr-luc

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pFR_HCV_xb

Plasmid

#11510

Depositor

Phil Sharp

Article

Petersen et al Mol Cell. 2006 Feb 17. 21(4):533-42.

Insert

Firefly luciferase, Hepatitis C Virus IRES sequence, Renilla, and 3'UTR short RNA binding sites

Use

Luciferase

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64

Plasmid

#203350

Purpose

Template for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

Depositor

Christopher Grigsby, Molly Stevens

Insert

Nano Luciferase

Use

Template for t7-mediated in vitro transcription o…

Tags

Flanked by 5' and 3' UTRs from Xenopus …

Promoter

T7

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pTNS3

Plasmid

#63127

Purpose

Tn7 transposase expression

Depositor

Herbert Schweizer

Article

Choi et al Appl Environ Microbiol. 2008 Feb;74(4):1

Type

Empty backbone

Expression

Bacterial

Promoter

P1 and Ptac

Available Since

May 17, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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