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pTW335f3

Plasmid

#237324

Purpose

expresses G. max RbcL RbcS-6xHis

Depositor

Tina Wang

Article

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Insert

G. max RbcL RbcS-6xHis

Tags

6xHis

Expression

Bacterial

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-crRNA-MS2

Plasmid

#207624

Purpose

Expresses crRNA-MS2 in mammalian cells (with a human-U6 promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

crRNA-MS2

Use

CRISPR

Promoter

hU6

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-BoxB-petracrRNA

Plasmid

#207625

Purpose

Expresses BoxB-petracrRNA in mammalian cells (with a human-U6 promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

BoxB-petracrRNA

Use

Synthetic Biology

Promoter

hU6

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-LambdaN-MCP

Plasmid

#207626

Purpose

Expresses LambdaN-MCP fusion protein in mammalian cells (with a CMV promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

LambdaN-MCP

Use

Synthetic Biology

Promoter

pCMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-ALFA-MCP

Plasmid

#207628

Purpose

Expresses ALFA-tagged MCP fusion protein in mammalian cells (with a CMV promoter)

Depositor

Jay Shendure

Article

Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

Insert

ALFA-MCP

Use

Synthetic Biology

Promoter

pCMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTW358c

Plasmid

#227934

Purpose

Express Aβ42 F19S/L34P-T7n

Depositor

Tina Wang

Article

Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

Insert

Aβ42-T7n

Expression

Bacterial

Mutation

F19S and L34P mutations in Aβ42

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1-p37-SHPmut-FLAG

Plasmid

#225502

Purpose

Inducible expression of FLAG-tagged mutated p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

FLAG

Expression

Mammalian

Mutation

F215A/E218A/QKL220-222AAA

Promoter

TRE promoter, Tet ON

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1-p37-WT-FLAG

Plasmid

#225501

Purpose

Inducible expression of FLAG-tagged wildtype p37 protein

Depositor

David Rubinsztein

Article

Wrobel et al Sci Adv. 2024 May 3;10(18):eadl6082. doi

Insert

p37 (UBXN2B Human)

Use

Lentiviral; Gateway destination vector, doxycycli…

Tags

FLAG

Expression

Mammalian

Mutation

no

Promoter

TRE promoter, Tet ON

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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LDB34

Plasmid

#185431

Purpose

Yeast expression of Nup1 C-terminal truncation (nup1-21 allele)

Depositor

Ken Belanger, Laura Davis

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Expression

Yeast

Mutation

Truncation at amino acid 1041 from the C-term of …

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB33

Plasmid

#185430

Purpose

Yeast expression of Nup1 N-terminal truncation (nup1-15 allele)

Depositor

Ken Belanger, Laura Davis

Article

Bogerd et al J Cell Biol. 1994 Oct . 127(2):319-32.

Insert

NUP1

Expression

Yeast

Mutation

Truncation of amino acids 3-191 from the N-term o…

Available Since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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CDW116

Plasmid

#129687

Purpose

transcription template, scrambled promoter

Depositor

Jeff Gelles

Article

Harden et al Nat Commun. 2020 Jan 23;11(1):448. doi:

Insert

synthetic transcription template

Expression

Bacterial

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-Craf SSAA

Plasmid

#118336

Purpose

Expression of human Craf

Depositor

Philip Stork

Article

Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

Insert

Craf SSAA (RAF1 Human)

Tags

Flag

Expression

Mammalian

Mutation

SSAA

Promoter

CMV

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pFS320-USP44-catDead

Plasmid

#43901

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Tags

MYC

Expression

Mammalian

Mutation

C282A, T91A

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pFS322-USP44-catDeadMyc

Plasmid

#43904

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Tags

HIS and MYC

Expression

Mammalian

Mutation

C282A, T91A

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pFS321-pDestMyc-USP44catDead

Plasmid

#43902

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Expression

Mammalian

Mutation

C282A

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pHR-SIN-GFP-PTEN-Y16C

Plasmid

#30542

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

GFP-PTEN-Y16C (PTEN Human)

Expression

Mammalian

Mutation

GFP-PTEN-Y16C

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-del(1-25)

Plasmid

#30390

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-del(1-25) (PTEN Human)

Expression

Mammalian

Mutation

del(1-25)

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-del(1-15)

Plasmid

#30389

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-del(1-15) (PTEN Human)

Expression

Mammalian

Mutation

del(1-15)

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-L25A

Plasmid

#30388

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-L25A (PTEN Human)

Expression

Mammalian

Mutation

L25A

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only
pHR-SIN-PTEN-D24N

Plasmid

#30387

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

Insert

PTEN-D24N (PTEN Human)

Expression

Mammalian

Mutation

D24N

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only