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pCDNA4.TO-ORF47-2xCSTREP

Plasmid

#136208

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF47

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF47

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF52-2xCSTREP

Plasmid

#136212

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF52

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF52

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF57-2xCSTREP

Plasmid

#136217

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF57

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF57

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-Ago2

Plasmid

#74231

Purpose

GFP-Ago2 lentiviral vector

Depositor

Carl Novina

Article

Janas et al RNA. 2012 Nov;18(11):2041-55. doi: 10.12

Insert

Ago2 (AGO2 Human)

Use

Lentiviral

Tags

eGFP

Available Since

April 6, 2016

Availability

Academic Institutions and Nonprofits only
pX330-RAB7A

Plasmid

#227297

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of RAB7A for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of RAB7A (RAB7A Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-moxGFP-Zeo-H2BC11

Plasmid

#207759

Purpose

Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

H2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 17, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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TMEM16F-I521K-peGFP-N1

Plasmid

#228588

Purpose

Expresses TMEM16F-I521K-peGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

I521K plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_antiCD19_eZ3_Gal4VP64

Plasmid

#169917

Purpose

Expression of a synNotch receptor containing antiCD19, a zebrafish Notch3 core with an additional EGF repeat, and Gal4VP64.

Depositor

Zhili Rong

Article

Huang et al Protein Cell. 2020 Apr;11(4):299-303. do

Insert

antiCD19-Notch3-GL4VP64

Use

Lentiviral

Expression

Mammalian

Mutation

Additional EGF repeat between the extracellular d…

Promoter

PGK

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pNeuLite

Plasmid

#16247

Depositor

Mien-Chie Hung

Article

Xing et al Nat Med. 2000 Feb . 6(2):189-95.

Insert

Her-2/neu promoter (ERBB2 Human)

Tags

Luciferase

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pAcBac1-GFP(39TAG)

Plasmid

#217360

Purpose

Expression of reporter EGFP with a TAG stop codon at 39 aa position; can be packaged into bacmid

Depositor

Abhishek Chatterjee

Article

Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

Insert

Enhanced green fluorescent protein

Use

Baculoviral

Expression

Insect and Mammalian

Mutation

Y39TAG

Promoter

CMV

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-ChRmine-eYFP-Kv2.1-WPRE

Plasmid

#130997

Purpose

Double floxed soma-targeted ChRmine-eYFP under the control of Ef1a promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

EYFP-Kv2.1

Promoter

Ef1a

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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TMEM16F-I611K-peGFP-N1

Plasmid

#228581

Purpose

Expresses mouse TMEM16F-I611K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)

Expression

Mammalian

Mutation

I611K plus a 3 amino acid N-terminal truncation (…

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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CaMKIIa-mScarlet-I-P2A-2M-Qt-FLAG

Plasmid

#206956

Purpose

AAV plasmid for CaMKIIa driven co-expression of mScarlet-I and 2M-Qt-FLAG EMcapsulins

Depositor

Gil Westmeyer

Article

Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

Insert

mScarlet-I, MmMT3, QtEnc

Use

AAV

Tags

FLAG

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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SARS_COV2_ORF3a_HA_BASU

Plasmid

#153994

Purpose

Express Orf3a gene along with HA and BASU

Depositor

Paul Khavari

Article

Meyers et al PLoS Pathog. 2021 Oct 1;17(10):e1009412.

Insert

Orf3a (ORF3a Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO5d.SFFV.eGFP-miR30n

Plasmid

#90333

Purpose

miR30 based shMir knockdown

Depositor

Dirk Heckl

Article

Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

Inserts

eGFP
na
SFFV
miR30n

Use

Lentiviral

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGL410-BRN2p

Plasmid

#110733

Purpose

Luciferase reporter for the human BRN2 promoter

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

BRN2 promoter luciferase (POU3F2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-Intron-CterFlag-MePCE

Plasmid

#113549

Purpose

Mammalian expression plasmid that can be used with the Flp-In T-REx system.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

Flag

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mMyh9 - 2

Plasmid

#198492

Purpose

lentiviral stable expression of mMyh9 gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mMyh9 (Myh9 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mMyh9 - 1

Plasmid

#198491

Purpose

lentiviral stable expression of mMyh9 gRNA 1

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mMyh9 (Myh9 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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Designed VEGF-binding scFv G6des13

Plasmid

#110213

Purpose

Yeast Surface Display of G6des13

Depositor

Sarel Fleishman

Article

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

G6des13

Tags

cmyc tag

Expression

Yeast

Mutation

L A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95F

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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