We narrowed to 62,186 results for: cloning
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Plasmid#108708PurposeExpression of a StrepII-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsStrepII-3C protease cleavage siteExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only
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pJR255
Plasmid#78549PurposeFor expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. CMV driven mCherry visible marker.DepositorTypeEmpty backboneUseRNAiExpressionMammalianPromoterU6, CMVAvailable SinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICSL60038
Plasmid#245677PurposeLevel 0 Golden Gate partAtUbi10 terminator, GCTT - CGCTDepositorInsertAtUbi10 terminator
UseSynthetic BiologyMutationBsaI/BpiI sites removed by point-mutationAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
R777-E125 Hs.MAPK1
Plasmid#70409PurposeGateway ORF clone of human MAPK1 [NM_002745.4] with stop codon (for native or N-terminal fusions)DepositorInsertMAPK1 (MAPK1 Human)
UseGateway CloningAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E015 Hs.BRAF
Plasmid#70299PurposeGateway ORF clone of human BRAF [NM_004333.4] with stop codon (for native or N-terminal fusions)DepositorInsertBRAF (BRAF Human)
UseGateway CloningAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCVD315
Plasmid#63737PurposeDerivative of pKO-1 carrying the multiple cloning region from M13mp19RFDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pE-mApple L5L2
Plasmid#187690PurposeC-Term Gateway cloning tagDepositorInsertmApple
UseGateway CloningTagsmAppleMutationWTAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
fj89c07 #2 in pBK-CMV
Plasmid#44966PurposeThis is intended for use in riboprobe synthesis, not for cloning.DepositorInsertAngptl4
ExpressionBacterialPromoterCMVAvailable SinceAug. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGPI-SceI
Plasmid#32060PurposeConstruction of unmarked gene deletions. Suicide cloning vector designed to introduce a targeted I-SceI restriction site in the genome of BurkholderiaDepositorTypeEmpty backboneUseMutagenesis plasmidAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-RFP (p15A origin)
Plasmid#74090PurposepTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin
UseSynthetic BiologyExpressionBacterialPromoterR0010 Const. Promoter controlling RFPAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHACK-GAL4>QF2(newHA1)
Plasmid#104873PurposeHACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of insertsDepositorInsertT2A-QF2
UseCRISPRExpressionInsectAvailable SinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-BN
Plasmid#60502PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of BFP, rtTA and neomycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterTCE & RPBSAAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
BBa_J72113-BBa_J72152
Plasmid#40784DepositorInsertPhagemid + GFP
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDual_dsCas9_BFP
Plasmid#214679PurposeLentiviral expression vector for an inducible Cas9-P2A-BFP with two empty sgRNA cloning sitesDepositorTypeEmpty backboneUseCRISPR and LentiviralAvailable SinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCVL TAL(Nd154C63) SFFV Xba-Sal linker
Plasmid#50420PurposepTAL plasmid compatible with the Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.DepositorTypeEmpty backboneUseLentiviral and TALENExpressionMammalianPromoterSFFVAvailable SinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Blast
Plasmid#227267PurposeEmpty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICSL13009
Plasmid#245657PurposeLevel 0 Golden Gate part TrpC promoter, GGAG - CCATDepositorInsertTrpC promoter
UseSynthetic BiologyMutationBsaI/BpiI sites removed by point-mutationAvailable SinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pICSL13018
Plasmid#245662PurposeLevel 0 Golden Gate part Suc2 promoter, GGAG - CCATDepositorInsertSuc2 promoter
UseSynthetic BiologyMutationBsaI/BpiI sites removed by point-mutationAvailable SinceJan. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pICSL12055
Plasmid#245648PurposeLevel 0 Golden Gate part Suc2 promoter, GGAG - AATGDepositorInsertSuc2 promoter
UseSynthetic BiologyMutationBsaI/BpiI sites removed by point-mutationAvailable SinceJan. 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pICSL60006
Plasmid#245667PurposeLevel 0 Golden Gate part RbcS terminator, GCTT - CGCTDepositorInsertRbcS terminator
UseSynthetic BiologyMutationBsaI/BpiI sites removed by point-mutationAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only