We narrowed to 59,364 results for: cloning

1. pUC19/N-eCFP

   Plasmid

   #183155

   Purpose

   Plant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eCFP

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pePEmax-pPuro

   Plasmid

   #215688

   Purpose

   Cloning vector for Uni-Vector prime editing system

   Depositor

   Kou-Juey Wu

   Article

   Huang et al Biotechnol J. 2024 Feb;19(2):e2300353. d

   Insert

   PEmax
   

   Use

   Unspecified

   Promoter

   EFS promoter

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAtC

   Plasmid

   #78097

   Purpose

   Plant expression of Cas9 and gRNA empty backbone; Basta resistance

   Depositor

   Jin-Soo Kim

   Article

   Kim et al J Integr Plant Biol. 2016 Mar 4. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   NLS-HA

   Expression

   Plant

   Promoter

   U6

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-CT3G-CERP2-MG-IRES2-mNeonGreen

   Plasmid

   #175508

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE3G-mCMV

   Available Since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. bdSUMO-TAT-GFP-HiBit

   Plasmid

   #170991

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-TAT-muGFP-HiBiT
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. hGip-R

   Plasmid

   #14942

   Depositor

   Bernard Thorens

   Article

   Gremlich et al Diabetes. 1995 Oct . 44(10):1202-8.

   Insert

   Gip-R (GIPR Human)
   

   Expression

   Mammalian

   Mutation

   Short form of the receptor

   Available Since

   June 7, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pPV-EF1a-EGxxFP-iP-A

   Plasmid

   #204624

   Purpose

   Cloning vector for SSA vector (EGxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

   Depositor

   Akitsu Hotta

   Article

   Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. 2Bc-T MBP_BoMoC(ed)_F753A_6xH

   Plasmid

   #185710

   Purpose

   Recombinant B. mori truncated R2 RT expression

   Depositor

   Kathleen Collins

   Article

   Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

   Insert

   B. mori truncated R2 RT
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   Changed F753 to A

   Promoter

   T7

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAtC

   Plasmid

   #78098

   Purpose

   Plant expression of Cas9 and gRNA empty backbone; Hygro Resistance

   Depositor

   Jin-Soo Kim, Sang-Gyu Kim

   Article

   Kim et al J Integr Plant Biol. 2016 Mar 4. doi: 10

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Promoter

   U6

   Available Since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO GFP DNAJB9

    Plasmid

    #19505

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB9 (DNAJB9 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCPP3373

    Plasmid

    #128718

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

    Insert

    hopQ1-1
    

    Expression

    Bacterial

    Mutation

    Synonymous point mutation in the wobble position…

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223 NRF2

    Plasmid

    #118708

    Purpose

    entry clone for NRF2

    Depositor

    William Kaelin

    Article

    Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

    Insert

    NRF2
    

    Expression

    Mammalian

    Mutation

    wt

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-Dyskerin

    Plasmid

    #207577

    Purpose

    N-terminal mCherry-tag using SalI/EcoRI

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    generated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19/N-mChe

    Plasmid

    #183159

    Purpose

    Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of mCherry.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS hShh (CT#401)

    Plasmid

    #13996

    Depositor

    Cliff Tabin

    Article

    Marigo et al Genomics. 1995 Jul 1. 28(1):44-51.

    Insert

    Shh (SHH Human)
    

    Expression

    Bacterial

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5/FRT/TO GFP DNAJB1

    Plasmid

    #19495

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB1 (DNAJB1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. bdSUMO-pHD118-GFP-HiBit

    Plasmid

    #171015

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-pHD118-muGFP-HiBiT
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. bdSUMO-pHlip-GFP-HiBit

    Plasmid

    #171016

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-pHlip-muGFP-HiBiT
    

    Use

    Luciferase

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. BPK1520_puroR

    Plasmid

    #173901

    Purpose

    SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection

    Depositor

    Ronald Cohn

    Article

    Erwood et al Genome Res. 2019 Dec;29(12):2010-2019. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV/U6

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5/FRT/TO V5 HSPA2

    Plasmid

    #19512

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 2 (HSPA2 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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