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426Gal-FUS-1-501aa-YFP

Plasmid

#29601

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-501aa (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-501

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-373aa-YFP

Plasmid

#29598

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-373aa (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-373

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-170aa-YFP

Plasmid

#29596

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-170aa (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-170

Available Since

April 29, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-413aa-YFP

Plasmid

#29599

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-413aa (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-413

Available Since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
pRRLsin.PPT.CMV‐GFP‐Eps8 WT

Plasmid

#74922

Purpose

Lentiviral plasmid expressing Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Menna et al PLoS Biol. 2009 Jun 30;7(6):e1000138. do

Insert

Eps8 (Eps8 Mouse)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-101-526aa-YFP

Plasmid

#29603

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-101-526aa (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 101-526

Available Since

July 20, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-453aa-YFP

Plasmid

#29600

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-453aa (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-453

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-267-526aa-YFP

Plasmid

#29605

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-267-526aa (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 267-526

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-285-526aa-YFP

Plasmid

#29606

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-285-526aa (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 285-526

Available Since

Sept. 7, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-1-270aa-YFP

Plasmid

#29597

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-270aa (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 1-270

Available Since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
426Gal-FUS-368-526aa-YFP

Plasmid

#29607

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-368-526aa (FUS Human)

Tags

YFP

Expression

Yeast

Mutation

Contains FUS aa# 368-526

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
Jam2-AP-His

Plasmid

#71953

Purpose

Expresses the extracellular region of the JAM-2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam2 (Jam2 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
METTL3

Plasmid

#155725

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

METTL3 (METTL3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

H330Q compared to BC052244, no mutations compared…

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-Ef1a-hMGT-PGK-puro

Plasmid

#172394

Purpose

Lentiviral expression vector for human direct cardiac reprogramming

Depositor

Li Qian

Article

Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

Insert

Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)

Use

Lentiviral

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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GAPDH

Plasmid

#155622

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

GAPDH (GAPDH Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Jam2-Fc-His

Plasmid

#72079

Purpose

Expresses the extracellular region of the JAM-2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Jam2 (Jam2 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP C1-Eps8 ∆cap/∆bund

Plasmid

#74891

Purpose

mammalian expression of the capping and bundling activity mutant Eps8 fused to GFP

Depositor

Giorgio Scita

Article

Hertzog et al PLoS Biol. 2010 Jun 1;8(6):e1000387. doi

Insert

Eps8 (Eps8 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

V689D, L693D, L757A, K759A

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only
pCS2-Prom1-TEVp-TwinStrep-His / IRES2 / Pcdh21-TEVp-Myc-Flag

Plasmid

#210820

Purpose

Mammalian overexpression vector for polycistronic co-expression of C-terminally Strep and His tagged human Prom1s1 (WT) and C-terminally Myc and Flag tagged human Pchd21 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Inserts

Prominin 1 (PROM1 Human)
Protocadherin 21 (CDHR1 Human)

Tags

Myc, 3xFlag and TwinStrep, 10xHis

Expression

Mammalian

Promoter

CMV and CMV / IRES2

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac Dual His6-TEV-CUL2, Rbx1

Plasmid

#231000

Purpose

Dual expression vector containing mouse CUL2 and Rbx1 for protein puritification from SF9 insect cells

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Inserts

Rbx1 (Rbx1 Mouse)
CUL2 (Cul2 Mouse)

Tags

6x HIS

Expression

Insect

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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P25-COMP-blac-flag-his

Plasmid

#110991

Purpose

Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

Depositor

Gavin Wright

Article

Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

Insert

ookinete surface protein P25 (PF10_0303 Synthetic)

Tags

Exogenous signal peptide of mouse origin and rat …

Expression

Mammalian

Mutation

All NXT/S sites mutated to NXA, where X is any am…

Promoter

CMV

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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