Skip to main content
Addgene
Showing: 721 - 740 of 3009 results

  6. pS148∙CsR

    Plasmid
    #197858
    Purpose
    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
    Depositor
    Susanne Häußler
    Article
    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41
    Insert
    gRNA scaffold - Ap/Cb resistance cassette - incP oriT
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    PEM7
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197858-plasmid-map-sequence-id-393255

    Enlarge

  7. LV-Let7d-5p

    Plasmid
    #117055
    Purpose
    Overexpression of Let-7d-5p using miR-451 backbone to enable DICER independent expression along with orange fluorescent protein (OFP) to track miRNA expression
    Depositor
    Michele De Palma
    Article
    Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d
    Insert
    Let-7d-5p - OFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    SFFV promoter
    Available since
    Oct. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117055-plasmid-map-sequence-id-223998

    Enlarge

  11. pCFJ2437 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid
    #159857
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Depositor
    Christian Froekjaer-Jensen
    Article
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
    Use
    Tags
    Expression
    Worm
    Mutation
    Not applicable
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159857-plasmid-map-sequence-id-318022

    Enlarge

  15. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

    Plasmid
    #128404
    Purpose
    Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli
    Depositor
    Michael Jewett
    Article
    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9
    Insert
    Exotoxin A (toxA P. aeruginosa)
    Use
    Tags
    6xHis tag and DsbA signal sequence for periplasmi…
    Expression
    Bacterial
    Mutation
    242DNNNS246 and 384DQNRT388 engineered glycosylat…
    Promoter
    trc
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    128404-plasmid-map-sequence-id-261690

    Enlarge

  17. pCFJ2452 - [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid
    #159854
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Depositor
    Christian Froekjaer-Jensen
    Article
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    [1-2] ENTR - Fluor - ce-mNeon(PATCs(900bp), NLS, no_atg, no stop)
    Use
    Tags
    Expression
    Worm
    Mutation
    Not applicable
    Promoter
    Available since
    Dec. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    159854-plasmid-map-sequence-id-318017

    Enlarge

  19. pCFJ2451 - [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid
    #159845
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Depositor
    Christian Froekjaer-Jensen
    Article
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)
    Use
    Tags
    Expression
    Worm
    Mutation
    Not applicable
    Promoter
    Available since
    Dec. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    159845-plasmid-map-sequence-id-317997

    Enlarge

  20. pCFJ2442 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid
    #159858
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Depositor
    Christian Froekjaer-Jensen
    Article
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
    Use
    Tags
    Expression
    Worm
    Mutation
    Not applicable
    Promoter
    Available since
    Dec. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    159858-plasmid-map-sequence-id-318023

    Enlarge

Showing: 721 - 740 of 3009 results