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pcDNA3.1 His-GFP-EWSR1-myc-His

Plasmid

#46385

Purpose

expresses EGFP tagged EWSR1 in mammalian cells

Depositor

Heinz Gehring

Article

Pahlich et al Proteins. 2008 Sep;72(4):1125-37. doi: 1

Insert

EWSR1 (EWSR1 Human)

Use

Tags

EGFP, His, and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC13A5

Plasmid

#131945

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC13A5 (SLC13A5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3HA-AR-LBD

Plasmid

#89111

Purpose

mammalian expression of human androgen receptor ligand binding domain amino acid residues 624-919 with N-terminal double HA tag

Depositor

Elizabeth Wilson

Article

He et al J Biol Chem. 1999 Dec 24;274(52):37219-2

Insert

hAR ligand binding domain (AR Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

contains Ligand Binding Domain (624-919)

Promoter

CMV

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-GFP-IMP2-2_antisense-control

Plasmid

#42174

Depositor

Alexandra Kiemer

Article

Kessler et al Am J Physiol Gastrointest Liver Physiol.

Insert

Insulin-like growth factor 2 mRNA binding protein 2-2 antisense control (IGF2BP2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

T7

Available since

June 14, 2013

Availability

Academic Institutions and Nonprofits only
pGL2-Mcl-1 mut

Plasmid

#19133

Depositor

Wafik El-Deiry

Article

Ricci et al Cancer Cell. 2007 Jul . 12(1):66-80.

Insert

Mcl-1 promoter (MCL1 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

NFkB binding-site mutant, the sequence GGGGTCTTCC…

Promoter

Available since

Oct. 2, 2008

Availability

Academic Institutions and Nonprofits only

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CMV-FFluc-GPD1L-3'UTR Full mut

Plasmid

#32489

Depositor

Pere Puigserver

Article

Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

Insert

GPD1L 3'UTR (Gpd1l Mouse)

Use

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated in the entire miR-210 binding region

Promoter

CMV

Available since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only
pMeCP2G pc1121

Plasmid

#167566

Purpose

The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced GFP (isolated from pEGFP-N1 vector; CLONTECH Laboratories, Inc.)

Depositor

Cristina Cardoso

Article

Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

Inserts

Methyl-CpG binding protein 2 (Mecp2 Rat)
GFP

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-DmrB-DmrB-Ripk3-2A-mCherry-puro

Plasmid

#231972

Purpose

Tet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosis

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

Ripk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGS_128

Plasmid

#160651

Purpose

Expresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

Depositor

Li-Huei Tsai, Susan Lindquist

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e3 (APOE Human)

Use

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e3 allele

Promoter

pZ estradiol inducible promoter

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pGS_129

Plasmid

#160652

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

Depositor

Li-Huei Tsai, Susan Lindquist

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Use

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

pZ estradiol inducible promoter

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pRetroX GFP Mps1 wt

Plasmid

#63702

Purpose

Inducible expression of GFP-Mps1 fusion in mammalian cells

Depositor

Floris Foijer

Article

Foijer et al Proc Natl Acad Sci U S A. 2014 Sep 16;11

Insert

Mps1 (TTK Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

tight TRE promoter

Available since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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HA-HA-AR-DBD-LBD

Plasmid

#171237

Purpose

Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain and Ligand-binding domain (DBD-LBD)

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

2xHA-tagged human androgen receptor trancate:DNA-binding domain and Ligand-binding domain (AR Human)

Use

Tags

HA

Expression

Bacterial and Mammalian

Mutation

AR truncate

Promoter

CMV

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC13A5_STOP

Plasmid

#161111

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC13A5 (SLC13A5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D

Plasmid

#110300

Purpose

Expresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1

Depositor

Chris Kok-Lung Chan

Article

Addis Jones et al Nat Commun. 2019 Jun 28;10(1):2861. doi:

Insert

p53-binding protein 1 (TP53BP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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V5-ERG-R367K

Plasmid

#118624

Purpose

Expresses V5 tagged ERG R367K (DNA binding mutant) protein

Depositor

William Hahn

Article

Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

Insert

ERG (R367K DNA binding mutant) (ERG Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

R367K

Promoter

EF1a

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX MLKL(1-180)-2A-mCherry-puro

Plasmid

#231976

Purpose

Tet inducible expression of DmrB-Mlkl with mCherry to induce MLKL 1-180 necroptosis

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

MLKL 1-180 construct with bi-cistronic mCherry (Mlkl Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX MLKL(S345D)-2A-mCherry-puro

Plasmid

#231975

Purpose

Tet inducible expression of DmrB-Mlkl with mCherry to induce phosphomimetic MLKL S345D necroptosis

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

MLKL S345D construct with bi-cistronic mCherry (Mlkl Mouse)

Use

Lentiviral

Tags

Expression

Mutation

MLKL S345D

Promoter

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-DmrB-Casp8-2A-mCherry-puro

Plasmid

#231971

Purpose

Tet inducible expression of DmrB-Casp8 with mCherry to induce Casp8 apoptosis

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

Casp8 dimerizing construct with bi-cistronic mCherry (CASP8 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-EWS_RRM(359-447)

Plasmid

#188045

Purpose

Expresses His8-EWS_RRM(359-447) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Biopolymers. 2023 May;114(5):e23536. doi

Insert

EWSR1 (EWSR1 Human)

Use

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-358, 446-656

Promoter

T7

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-EWS_RRM-RGG(359-513)

Plasmid

#188046

Purpose

Expresses His8-EWS_RRM-RGG(359-513) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Biopolymers. 2023 May;114(5):e23536. doi

Insert

EWSR1 (EWSR1 Human)

Use

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-358, 514-656

Promoter

T7

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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