Addgene

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HEK4-53bp-tag-insertion-pegRNA

Plasmid

#227673

Purpose

PegRNA for 53 bp insertion

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

SpCas9 sgRNA scaffold (EPHA3 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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eIF3a- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192236

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3a

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3a-knockin-homology arm-mAID-mCherry2-Hygro (EIF3A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3a- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192235

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3a

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3a-knockin-homology arm-mAID-mClover-NeoR (EIF3A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3k- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192243

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3k

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3k-knockin-homology arm-mAID-mClover-NeoR (EIF3K Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3k- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192244

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3k

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3k-knockin-homology arm-mAID-mCherry2-Hygro (EIF3K Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3b- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192237

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3b

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3b-knockin-homology arm-mAID-mClover-NeoR (EIF3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3b- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192238

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3b

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3b-knockin-homology arm-mAID-mCherry2-Hygro (EIF3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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PB-TAC-OSKM

Plasmid

#80481

Purpose

piggyBac transposon for dox-inducible expression of the OSKM polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OSKM (Pou5f1, Sox2, Klf4, Myc Mouse)

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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PB-TAC-MKOS

Plasmid

#80484

Purpose

piggyBac transposon for dox-inducible expression of the MKOS polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

MKOS (Myc, Klf4, Pou5f1, Sox2 Mouse)

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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PB-TAC-OKMS

Plasmid

#80480

Purpose

piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OKMS (Pou5f1, Klf4, Myc, Sox2 Mouse)

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Klf4 [10-483]

Promoter

tetO

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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PB-TET-MKOS

Plasmid

#20959

Purpose

piggyBac vector with tet inducible cMyc, KLF4, Oct4, Sox2 for creation of induced pluripotent stem cells

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

cMyc, KLF4, Oct4, Sox2 (Myc, Klf4, Pou5f1, Sox2 Mouse)

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2009

Availability

Academic Institutions and Nonprofits only

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PB-TAC-OK+9MS

Plasmid

#80482

Purpose

piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OK+9MS (Pou5f1, Klf4, Myc, Sox2 Mouse)

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Klf4 [1-483]

Promoter

tetO

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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PB-TAC-EB-C5

Plasmid

#80485

Purpose

piggyBac transposon for dox-inducible expression of the EB-C5 polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

lin-28 homolog A (C. elegans) (Pou5f1, Sox2, Klf4, Myc, Lin28a Mouse)

Use

Piggybac transposon

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
plp

Plasmid

#22310

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Type

Empty backbone

Use

Integrating plasmid

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pRS403-PGAL1-strongSC_GFP

Plasmid

#22316

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

GFP-rad9-GFP

Use

Integrating plasmid

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pYES2.1-Dcr1

Plasmid

#22307

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

DCR1

Use

2micron

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pYES2.1-Ago1

Plasmid

#22306

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

AGO1

Use

2micron

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pRS403-PGAL1-weakSC_GFP

Plasmid

#22315

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

GFP-rad9

Use

Integrating plasmid

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pET101-Dcr1

Plasmid

#22309

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

DCR1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only
plp-strongSC_GFP

Plasmid

#22312

Depositor

David Bartel

Article

Drinnenberg et al Science. 2009 Sep 10. ():.

Insert

GFP-rad9-GFP

Use

Integrating plasmid

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge