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pcDNA5-FRT-TO-Nsp8-HF

Plasmid

#157696

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp8-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp12-HF

Plasmid

#157711

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp12-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA5-FRT-TO-Nsp1-HF

Plasmid

#157681

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

HF-Nsp1 (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp7-HF

Plasmid

#157690

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp7-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA5-FRT-TO-Nsp15-HF

Plasmid

#157722

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp15 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp15-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA5-FRT-TO-Nsp2-HF

Plasmid

#157684

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp2-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(D256A/PAMmut)

Plasmid

#176141

Purpose

EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutation Asp256Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

XRCC1-T2A-PolB (XRCC1, POLB Human)

Use

Lentiviral

Tags

EGFP and MYC

Expression

Mammalian

Mutation

Myc-tagged PolB with mutation in Asp256 to Ala, a…

Promoter

EF1A

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSDH60

Plasmid

#139029

Purpose

MosSCI SapTrap assembly: Cassette 1 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

spe-11 promoter (spe-11 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pXF121

Plasmid

#139028

Purpose

MosSCI SapTrap assembly: Cassette 1 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

short mex-5 promoter (mex-5 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSDH54

Plasmid

#139052

Purpose

MosSCI SapTrap assembly: Cassette 4 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

tbb-2 3'UTR (tbb-2 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pXF276

Plasmid

#139048

Purpose

MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

lov domain (includes STOP codon)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pXF85

Plasmid

#139051

Purpose

MosSCI SapTrap assembly: Cassette 4 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

pie-1 3'UTR (pie-1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pXF262

Plasmid

#139038

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

cox-4 (no STOP codon) (W09C5.8 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSDH50

Plasmid

#139037

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

tomm-20 (1-55 a.a.) (no STOP codon) (tomm-20 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pJF7

Plasmid

#139036

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

tomm-20 (no STOP codon) (tomm-20 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSDH66

Plasmid

#139053

Purpose

MosSCI SapTrap assembly: Cassette 4 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

unc-54 3'UTR (unc-54 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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Xalb_I-C_array_GG

Plasmid

#178767

Purpose

Golden Gate vector to generate X. albilineans type I-C single arrays

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

Golden Gate vector BaeI recognition site to generate X. albilineans type I-C single arrays

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGWB517

Plasmid

#74859

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB519

Plasmid

#74861

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB516

Plasmid

#74858

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge