Addgene

We narrowed to 1,942 results for: eda

Showing: 721 - 740 of 1942 results

Results per page:

Sort results by:

pMpGWB116

Plasmid

#68570

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Nuclear localization signal-tagged tdTomato (tdTo…

Expression

Plant

Mutation

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCG-TPMV Hd32-IL-13

Plasmid

#163869

Purpose

Expresses TPMV H glycoprotein bearing IL-13 in mammalian cells

Depositor

Jakob Reiser

Article

Argaw et al Mol Ther Methods Clin Dev. 2021 Apr 24;2

Insert

TPMV H glycoprotein/IL-13

Use

Tags

Human IL-13

Expression

Mammalian

Mutation

Amino acids 2 to 33 at N terminus deleted

Promoter

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CaMP0.4-FHS-paCaMKII-WPRE3

Plasmid

#165429

Purpose

Expresses photoactivatable CaMKII in mammalian cells to induce synaptic plasticity.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

FHS-paCaMKII

Use

AAV

Tags

FHS(Flag, HisX6, Strep) tag

Expression

Mammalian

Mutation

Promoter

CaMKII0.4

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINB-OTUB1*

Plasmid

#65441

Purpose

Expresses activated OTUB1 in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Inserts

UbcH5b (UBE2D2 Human)
otubain-1 (OTUB1 )

Use

Tags

His-3C

Expression

Bacterial

Mutation

Active site cysteine mutated to alanine

Promoter

T7

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only
pMpGWB215

Plasmid

#68606

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Use

Tags

Nuclear localization signal-tagged Citrine (Citri…

Expression

Plant

Mutation

Promoter

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
L1_lacZgRNA-Ck3

Plasmid

#136136

Purpose

L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
L1_Cas9-Ck4

Plasmid

#136135

Purpose

L1 in position 4, for AtcoCas9 (Puchta) expression in L2 CRISPR vectors

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpEF1a:Cas9_3tNos-35S

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV.CMV.flex.hM3Dq.Cherry

Plasmid

#170360

Purpose

Cre-inducible lentiviral vector expressing hM3Dq

Depositor

Johan Jakobsson

Article

Klawonn et al Immunity. 2021 Feb 9;54(2):225-234.e6. d

Insert

flex.hM3Dq.Cherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
L1_lacZgRNA-Ck2

Plasmid

#136137

Purpose

L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CMV-GFP-2A-G(N2c)-WPRE

Plasmid

#194352

Purpose

Lentivirus expressing eGFP and Rabies Glycoprotein (G) from CVS-N2c strain

Depositor

Marco Tripodi

Article

Lee et al Nat Methods. 2023 Apr;20(4):580-589. doi

Inserts

Enhanced Green Fluorescent Protein (eGFP)
Rabies CVS-N2c glycoprotein (G)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pClneoMyc mouse LNX2

Plasmid

#37010

Depositor

Yutaka Hata

Article

Kansaku et al Oncogene. 2006 Aug 24;25(37):5071-84. Ep

Insert

LNX2 (Lnx2 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2012

Availability

Academic Institutions and Nonprofits only
pLVX-Tight-Puro_Nkx2.2

Plasmid

#64840

Purpose

A lentiviral vector for the expression of mouse Nkx2.2 under doxycyline control.

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Nkx2.2 (Nkx2-2 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Tight-Puro_MYRF

Plasmid

#64843

Purpose

A lentiviral vector for the expression of mouse MYRF under doxycyline control.

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

MYRF (Myrf Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pOPINS-AREL1

Plasmid

#66710

Purpose

Expresses AREL1 HECT domain in E. coli

Depositor

David Komander

Article

Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

Insert

KIAA0317 (AREL1 )

Use

Tags

His-SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only
CMV-tdTomato-P2A-paCaMKII(K42M)

Plasmid

#165433

Purpose

Expresses tdTomato and photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

tdTomato-P2A-paCaMKII(K42M)

Use

Tags

tdTomato

Expression

Mammalian

Mutation

K42M: Kinase dead mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221_SLC22A1

Plasmid

#132034

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

Insert

SLC22A1 (SLC22A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLVX-Tight-Puro_Olig2

Plasmid

#64842

Purpose

A lentiviral vector for the expression of mouse Olig2 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Olig2 (Olig2 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PROM_MpUBE2

Plasmid

#136109

Purpose

PROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous expression in early satges of gemmae development.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

PROM_MpUBE2 Mapoly0106s0019

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
5UTR_MpUBE2

Plasmid

#136110

Purpose

5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development.

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

5UTR_MpUBE2 Mapoly0106s0019

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLVX-Tight-Puro_ST18

Plasmid

#64844

Purpose

A lentiviral vector for the expression of mouse ST18 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

ST18 (St18 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge