We narrowed to 5,293 results for: pcDNA3.1+

1. pcDNA3.1 FLAG-BACH2

   Plasmid

   #232275

   Purpose

   Expression of FLAG-tagged BACH2 (H. sapiens) in human cell lines

   Depositor

   Michele Pagano

   Article

   Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

   Insert

   BACH2 (BACH2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_iSH2-pMag(3x)-iRFP

   Plasmid

   #67304

   Purpose

   Blue light dependent photoswitching system

   Depositor

   Moritoshi Sato

   Article

   Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

   Insert

   iSH2-pMag(3x)-iRFP (PIK3R1 Human, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-hSTING (N154S)

   Plasmid

   #214160

   Purpose

   Transient expression of gain-of-function STING mutant

   Depositor

   Nan Yan

   Article

   Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

   Insert

   STING (N154S) (STING1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N154S

   Promoter

   CMV

   Available since

   March 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-3C-sGFP1-10_pcDNA3.1(-)

   Plasmid

   #182931

   Purpose

   For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

   Depositor

   William Weis

   Article

   Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

   Type

   Empty backbone

   Use

   Tags

   3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCDNA3.1-ADGRL3-Flag

   Plasmid

   #201218

   Purpose

   pCDNA3.1 with kozak (GCC), ADGRL3 and C-terminal Flag

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(-)-flag-UBR2

   Plasmid

   #50573

   Purpose

   Expresses FLAG-tagged mouse UBR2 in mammalian cells.

   Depositor

   Alexander Varshavsky

   Insert

   UBR2 (Ubr2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

7. His-Precission-NEDD4L_C942S_pCDNA3.1(+)

   Plasmid

   #187729

   Purpose

   Express the catalytically inactive NEDD4L FL in mammalian cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L FL with C874 mutated to a serine (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Mammalian

   Mutation

   Cys874 mutated to a serine

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-mArg1-Flag

   Plasmid

   #34574

   Depositor

   Peter Murray

   Insert

   Arg1 (Arg1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1-FGFR4-V5/HIS

   Plasmid

   #201109

   Purpose

   expression of human FGFR4 receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human FGFR4 receptor tyrosine kinase, full length, wildtype (FGFR4 Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)Gαi3-91-NLuc

    Plasmid

    #207150

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Dominique Schols, Tom Van Loy

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAI3 (GNAI3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)K

    Promoter

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 CLN3-GFP

    Plasmid

    #78110

    Purpose

    Mammalian expression of human CLN3 with C terminal GFP fusion

    Depositor

    Thomas Braulke

    Insert

    CLN3 (CLN3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

12. Dead-Laconic/pcDNA3.1(-)

    Plasmid

    #118627

    Purpose

    To express in mammalian cells a lactate FRET sensor negative control.

    Depositor

    Luis Felipe Barros

    Article

    Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

    Insert

    Dead-Laconic
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Change Histidine 151 to Aspartic Acid of Escheric…

    Promoter

    CMV Promoter

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-Cry2FL-VP64

    Plasmid

    #60554

    Purpose

    Expresses Cry2FL-VP64 in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    Cry2FL-VP64
    

    Use

    Tags

    FLAG, HA tag, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1+/-FLI1-C‐(K)‐DYK

    Plasmid

    #129497

    Purpose

    Mammalian expression of human FLI1

    Depositor

    Marjorie Brand

    Article

    Palii et al Cell Stem Cell. 2019 May 2;24(5):812-820

    Insert

    FLI1 (FLI1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-hRIPK1-Flag

    Plasmid

    #112487

    Purpose

    Mammalian expression of Flag-hRIPK1. Please note Flag tag is N-terminal.

    Depositor

    Junying Yuan

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA3.1-Flag-H2A

    Plasmid

    #63560

    Purpose

    Expression of human H2A tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC13 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-IDH2-R172K

    Plasmid

    #87927

    Purpose

    Expresses IDH2 (R172K mutant) in mammalian cells

    Depositor

    Reproducibility Project Cancer Biology

    Article

    Showalter et al Elife. 2017 Jun 27;6. pii: e26030. doi:

    Insert

    isocitrate dehydrogenase (NADP(+)) 2, mitochondrial (IDH2 Human)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    R172K

    Promoter

    CMV

    Available since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1_iSH2-pMagFast2(3x)-iRFP

    Plasmid

    #67298

    Purpose

    Blue light dependent photoswitching system

    Depositor

    Moritoshi Sato

    Article

    Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

    Insert

    iSH2-pMagFast2(3x)-iRFP (PIK3R1 Human, Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1/hChR2-EYFP

    Plasmid

    #20939

    Purpose

    Mammalian expression of humanized ChR2 fused to EYFP

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-GGamma8-GFP2

    Plasmid

    #140990

    Purpose

    Encodes a G gamma subunit (GNG8) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GGamma8-GFP2 (GNG8 Human)
    

    Use

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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