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pENTR_hhN-HA

Plasmid

#240225

Purpose

Gateway entry clone with N-terminal hedgehog (hhN) tagged with HA (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

hedgehog N-terminal fragment (hh Fly)

Use

Gateway shuttle vector

Tags

HA-spGFP11

Mutation

aa 1-257

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hXIAP_1k)-PGKpuro2ABFP-W

Plasmid

#208420

Purpose

Lentiviral gRNA plasmid targeting human XIAP gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

XIAP (XIAP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hXIAP_2b)-PGKpuro2ABFP-W

Plasmid

#208421

Purpose

Lentiviral gRNA plasmid targeting human XIAP gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

XIAP (XIAP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hRIPK3_1k)-PGKpuro2ABFP-W

Plasmid

#208424

Purpose

Lentiviral gRNA plasmid targeting human RIPK3 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

RIPK3 (Ripk3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hRIPK3_2b)-PGKpuro2ABFP-W

Plasmid

#208425

Purpose

Lentiviral gRNA plasmid targeting human RIPK3 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

RIPK3 (Ripk3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV24-1

Plasmid

#237945

Purpose

Encodes TRBV24-1 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV24-1-TRAC (TRBV24-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV39

Plasmid

#237993

Purpose

Encodes TRAV39 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV39 (TRAV39 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV40

Plasmid

#237994

Purpose

Encodes TRAV40 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV40 (TRAV40 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV28

Plasmid

#237948

Purpose

Encodes TRBV28 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV28-TRAC (TRBV28 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV30

Plasmid

#237950

Purpose

Encodes TRBV30 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV30-TRAC (TRBV30 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV24

Plasmid

#237981

Purpose

Encodes TRAV24 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV24 (TRAV24 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV26-1

Plasmid

#237983

Purpose

Encodes TRAV26-1 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV26-1 (TRAV26-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV11-3

Plasmid

#237934

Purpose

Encodes TRBV11-3 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV11-3-TRAC (TRBV11-3 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV12-4

Plasmid

#237936

Purpose

Encodes TRBV12-4 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV12-4-TRAC (TRBV12-4 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV13

Plasmid

#237938

Purpose

Encodes TRBV13 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV13-TRAC (TRBV13 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRBV12-5

Plasmid

#237937

Purpose

Encodes TRBV12-5 allele and TRAC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBV12-5-TRAC (TRBV12-5 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV16

Plasmid

#237973

Purpose

Encodes TRAV16 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV16 (TRAV16 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV17

Plasmid

#237974

Purpose

Encodes TRAV17 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV17 (TRAV17 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV13-1

Plasmid

#237970

Purpose

Encodes TRAV13-1 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV13-1 (TRAV13-1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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TRAV13-2

Plasmid

#237971

Purpose

Encodes TRAV13-2 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV13-2 (TRAV13-2 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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