We narrowed to 36,304 results for: NAM

1. pTXB1-TP1

   Plasmid

   #160084

   Purpose

   Bacterial expression of Tn5-APEX2 fusion protein, no linker

   Depositor

   Pier Pandolfi

   Article

   Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

   Insert

   Tn5 transposase/APEX2 peroxidase fusion protein
   

   Use

   Tags

   FLAG and Mxe intein - Chitin-binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTXB1-TP2

   Plasmid

   #160085

   Purpose

   Bacterial expression of Tn5-APEX2 fusion protein with linker PAPAP

   Depositor

   Pier Pandolfi

   Article

   Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

   Insert

   Tn5 transposase/APEX2 peroxidase fusion protein
   

   Use

   Tags

   FLAG and Mxe intein - Chitin-binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDEST53-Arl13b20

   Plasmid

   #40876

   Depositor

   Tamara Caspary

   Article

   Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

   Insert

   Arl13b (Arl13b Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   deleted Arl N-terminal domain; added stop codon

   Promoter

   CMV

   Available since

   Nov. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

4. ROZA-XL YF

   Plasmid

   #64195

   Purpose

   Fluorescent reporter for ZAP-70 tyrosine kinase activity- Y to F mutated control sequence

   Depositor

   Annemarie Lellouch

   Article

   Cadra et al Biochem Biophys Res Commun. 2015 Feb 28.

   Insert

   ROZA-XL YF
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   ROZA-XL tyrosine phosphorylation site mutated to …

   Promoter

   CMV

   Available since

   April 23, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pOPTXGARE

   Plasmid

   #68542

   Purpose

   cyclic nucleotide reporter for bacteria or plant cells

   Depositor

   Helen Irving

   Article

   Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

   Insert

   OPTX promoter with GARE (AT1G33440 Mustard Weed)
   

   Use

   Tags

   luciferase

   Expression

   Bacterial and Plant

   Mutation

   5x GARE inserted 190 bp 5' pf ATG (GARE = ta…

   Promoter

   OPTX promoter with GARE

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNB785

   Plasmid

   #60164

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus-PEST
   

   Use

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   SIC1pr

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-mCherry-LKB1(S431A)

   Plasmid

   #84641

   Purpose

   mCherry-tagged, LKB1(S431A); lacks C-terminal PKA phosphorylation site

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   mCherry-LBK1(S431A) (STK11 Synthetic, Human)
   

   Use

   Tags

   6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

   Expression

   Mammalian

   Mutation

   PKA phosphorylation site Serine 428 mutated to Al…

   Promoter

   CMV

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNB787

   Plasmid

   #60152

   Purpose

   Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus-PEST
   

   Use

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   LEU1pr

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNB789

   Plasmid

   #60166

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus-PEST
   

   Use

   Tags

   PEST degron

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   RNR1pr

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPD95.75-cYAP-1

    Plasmid

    #66857

    Purpose

    Generate transgenic C.elegans with cYAP-1 fused to GFP

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

    Insert

    C.elegans YAP-1 (yap-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Serine104 is mutated to alanine

    Promoter

    C. elegans 5'UTR

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only