We narrowed to 15,534 results for: LEA

1. AP2u2-CMV-S_protein

   Plasmid

   #226456

   Purpose

   Mammalian expression of S protein of SARS-CoV2 under CMV promoter, tagged with 6His and released extracellular environment after expression for protein purification

   Depositor

   Urartu Seker

   Article

   Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(

   Insert

   Spike Protein
   

   Use

   Tags

   6xHis Tag, Foldon Domain, and Thrombin Cleavage S…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBAD-WzxE-TEV-His10

   Plasmid

   #226781

   Purpose

   Expression of C-terminally TEV cleavable His10-tagged WzxE from E. coli in E. coli

   Depositor

   James Naismith

   Article

   Le Bas et al Open Biol. 2025 Jan;15(1):240310. doi: 1

   Insert

   wzxE
   

   Use

   Tags

   TEV cleavable 10xHistidine tag

   Expression

   Bacterial

   Mutation

   valine insertion in position 2 due to the cloning…

   Promoter

   araBAD

   Available since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-myc:Skp1(dC111):gs10:FRB:HA

   Plasmid

   #228532

   Purpose

   A fragment of human Skp1 protein in fusion with FRB domain through a flexible glycine-serine linker.

   Depositor

   Roman Jerala

   Article

   Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

   Insert

   myc:Skp1(dC111):gs10:FRB:HA (SKP1 Synthetic, Human)
   

   Use

   Tags

   HA and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cdk13_intron3_sg3_pX458

   Plasmid

   #127338

   Purpose

   sgRNA that cuts within intron 3 of mouse CDK13 genomic locus- guide #3

   Depositor

   Phil Sharp

   Article

   Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

   Insert

   Mouse Cdk13 Intron 3 sgRNA
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   U6 Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cdk13_intron4_sg4_pX330

   Plasmid

   #127341

   Purpose

   sgRNA that cuts within intron 4 of mouse CDK13 genomic locus- guide #4

   Depositor

   Phil Sharp

   Article

   Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

   Insert

   Mouse Cdk13 Intron 4 Targeting sgRNA
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   U6 Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMS2374

   Plasmid

   #225631

   Purpose

   High Copy number plasmid containing Ple391 (DRD2 MiniPromoter) insert

   Depositor

   Elizabeth Simpson

   Article

   de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

   Insert

   Ple391 (DRD2 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEMS2368

   Plasmid

   #225632

   Purpose

   High Copy number plasmid containing Ple385 (GPR6 MiniPromoter) insert

   Depositor

   Elizabeth Simpson

   Article

   de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

   Insert

   Ple385 (GPR6 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEMS2372

   Plasmid

   #225630

   Purpose

   High Copy number plasmid containing Ple389 (ADORA2A MiniPromoter) insert

   Depositor

   Elizabeth Simpson

   Article

   de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

   Insert

   Ple389 (ADORA2A Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcs2-cDELE1(142-515)-sumo

   Plasmid

   #226107

   Purpose

   cDELE1 (corresponding to mitochondrially imported, cleaved DELE1, residues 142-515) fused to sumo used for in-vitro translation by wheat germ extract in in-vitro ubiquitylation assays

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   DELE1 (DELE1 Human)
   

   Use

   Tags

   Sumo

   Expression

   Mammalian

   Mutation

   encodes only residues 142-515 of DELE1 (represent…

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKM-U6-reRNA-hsRUNX

    Plasmid

    #205639

    Purpose

    reRNA guide targeting Homo sapiens RUNX

    Depositor

    Gerald Schwank

    Article

    Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

    Insert

    reRNA-hsRUNX (RUNX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET41-FeoB-OL2del

    Plasmid

    #216742

    Purpose

    Study the interaction of FeoB in P. aeruginosa, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL2 in its interaction with exogenous peptides.

    Depositor

    Gordon Laurie

    Article

    Sharifian Gh et al J Biol Chem. 2025 May;301(5):108455. doi

    Insert

    feoB (PA4358 P. aeruginosa (Bacteria))
    

    Use

    Tags

    8x His

    Expression

    Bacterial

    Mutation

    Outer loop 2 (OL2) with the sequence of [LEDSGYMA…

    Promoter

    Available since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. mTph2p(330bp)-Luc

    Plasmid

    #223663

    Purpose

    Firefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoter

    Depositor

    Kyungjin Kim

    Article

    Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

    Insert

    mTph2 promoter (Tph2 Mouse)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    mTph2 promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSAVED-CHAT-cbm_PCaspase

    Plasmid

    #214047

    Purpose

    Bacterial expression plasmid for SAVED-CHAT cA3 binding pocket mutant and PCaspase from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-PCaspase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    K121E, Q122A, N274A, R276E, Y285A, F286A

    Promoter

    lacUV5 promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIG-LSL-LRRC37B

    Plasmid

    #220791

    Purpose

    To express the human receptor LRRC37B (cre-dependant) in mammalian cells together with EGFP

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

    Insert

    LRRC37B (LRRC37B Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCIG-LRRC37B-HA

    Plasmid

    #220792

    Purpose

    To express the human receptor LRRC37B HA-tagged in mammalian cells together with EGFP

    Depositor

    Pierre Vanderhaeghen

    Article

    Libe-Philippot et al Cell. 2023 Dec 21;186(26):5766-5783.e25.

    Insert

    LRRC37B (LRRC37B Human)
    

    Use

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOTTC1789 - pAAV (flox-stop) mGrid1 390F gRNA A EF1a eGFP-KASH

    Plasmid

    #131684

    Purpose

    An adeno-associated viral vector expressing nuclear envelope-embedded eGFP and a Cre-dependent guide RNA for mGrid1

    Depositor

    Christopher Richie

    Article

    Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

    Inserts

    EGFP-KASH
    SpCas9 sgRNA vs mouse GRID1
    

    Use

    AAV

    Tags

    KASH

    Expression

    Mutation

    Promoter

    EF1a and mU6-LSL (Cre dependent)

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSAVED-CHAT_PCaspase-N-C-term

    Plasmid

    #214046

    Purpose

    Bacterial expression plasmid for SAVED-CHAT, PCaspase N-terminal fragment (aa 1-153), and C-terminal fragment (154-666) from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-PCaspase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    aa 1-153, 154-666

    Promoter

    lacUV5 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSAVED-CHAT-sm_PCaspase

    Plasmid

    #214048

    Purpose

    Bacterial expression plasmid for SAVED-CHAT CHAT-CHAT singlet interface mutant and PCaspase from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-PCaspase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R456E, D459K, D476K, R479E

    Promoter

    lacUV5 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJS-BCD-Strep-SAVED-CHAT

    Plasmid

    #214030

    Purpose

    Bacterial expression plasmid for strep-tagged wild-type SAVED-CHAT from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJS-BCD-Strep-dSAVED-CHAT

    Plasmid

    #214031

    Purpose

    Bacterial expression plasmid for strep-tagged catalytically dead SAVED-CHAT mutant from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    H375A, C422A

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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