We narrowed to 11,255 results for: nar;

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mCh-Drp1

Plasmid

#49152

Purpose

mammalian expression of Drp1 fused to mCherry

Depositor

Gia Voeltz

Article

Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

Insert

Drp1 (DNM1L Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8f

Plasmid

#162373

Purpose

Mammalian expression of ultrafast protein calcium sensor

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8f

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

CMV

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV/D377Y-mPCSK9

Plasmid

#58376

Purpose

Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

Depositor

Jacob Bentzon

Article

Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

Has Service

AAV8

Insert

proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)

Use

AAV

Expression

Mammalian

Mutation

D377Y

Promoter

HCRApoE/hAAT

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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DsRed-rab11 WT

Plasmid

#12679

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Tags

DsRed and HA

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-jRCaMP1a

Plasmid

#61562

Purpose

Red fluorescent calcium sensor protein

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Insert

NES-jRCaMP1a

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-SPOTlight-U2GCR

Plasmid

#164819

Purpose

Reporter to measure ISR state-dependent protein synthesis

Depositor

Nicole Calakos

Article

Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

Insert

SPOTlight-U2GCR

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCCLc-MND-A0201-SABR-Backbone

Plasmid

#119050

Purpose

3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

Depositor

David Baltimore

Article

Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

MND

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCCLc-MND-B2705-SABR-Backbone

Plasmid

#119051

Purpose

3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

Depositor

David Baltimore

Article

Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAY15 (OsTIR1(F74G) mAID-EGFP-NLS)

Plasmid

#140534

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-NLS TOL2

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G) mAID-EGFP-NLS TOL2

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES-jRGECO1a.WPRE.SV40

Plasmid

#100854

Purpose

AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

Insert

jRGECO1a

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)

Plasmid

#140659

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-Nluc

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G) mAID-EGFP-Nluc TOL2

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-Tet-OsTIR1(F74G)-V5

Plasmid

#158664

Purpose

Tet-OsTIR1(F74G)-V5 donor for integration at the AAVS1 locus

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

Tet-OsTIR1(F74G) AAVS1

Use

CRISPR

Expression

Mammalian

Mutation

F74G

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.LSL.tdTomato

Plasmid

#100048

Purpose

AAV mediated Cre-dependent expression of tdtomato (Lox-Stop-Lox)

Depositor

Hongkui Zeng

Article

Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

Insert

tdtomato

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-rab7 WT

Plasmid

#12605

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Tags

GFP and HA

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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6His-MBP-TEV-huAsCpf1

Plasmid

#90095

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huAsCpf1

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV4a-Flag-c-Myc

Plasmid

#102625

Purpose

Expresses c-Myc in mammalian cells

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

MYC (MYC Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-3XFLAG-hINO80

Plasmid

#44149

Depositor

Joan Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

hINO80 full length (INO80 Human)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-Rab27B

Plasmid

#89447

Purpose

Expression of GFP-tagged human Rab27B in mammalian cells

Depositor

Wendy Westbroek

Article

Hendrix et al J Natl Cancer Inst. 2010 Jun 16;102(12):

Insert

human Rab27B (RAB27B Human)

Tags

eGFP

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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(559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

Plasmid

#163024

Purpose

Expression of CRISPRi with U6-gRNA (Bsa1 sites)

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

AAV

Tags

KRAB, SV40 polyA, and myc NLS

Expression

Mammalian

Promoter

hEF-1a

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-CFP;UBC-Cre25nt

Plasmid

#65727

Purpose

Expresses Cre recombinase with a 25 nucleotide extracellular vesicle targeting sequence under the UBC promotor and CFP under the CMV promotor.

Depositor

Jacco van Rheenen

Article

Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

Inserts

CMV promoter driving CFP expression
UBC promoter driving Cre-25nt expression

Use

Cre/Lox

Expression

Mammalian

Promoter

CMV and UBC

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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