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pB-CAGGS-dCas9-KRAB

Plasmid

#110822

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pJT305 Pa01 Lenti pEF-attB LSR GFP

Plasmid

#184948

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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dCas9-KRAB

Plasmid

#110820

Purpose

Conventional dCas9 repressor-dCas9-KRAB

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CMV

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-SNAP-Rab6a_T27N

Plasmid

#248794

Purpose

Expresses SNAP-Rab6a-T27N (dominant negative) in mammalian cells. Generated from Addgene #46782 plasmid.

Depositor

Michael Robichaux

Article

Hekmatara et al bioRxiv [Preprint]. 2025 Sep 25:2025.09.

Insert

Rab6a (RAB6A Human)

Tags

SNAPf

Expression

Mammalian

Mutation

Change T 27 to N

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pJT301 BxBi Lenti pEF-attB LSR GFP

Plasmid

#184944

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

BxBi Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCDF-His-mTET1CD

Plasmid

#81053

Purpose

bacterial expression of the catalytic domain of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Mutation

catalytic domain amino acid 1367-2057

Promoter

T7

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pJT306 Kp03 Lenti pEF-attB LSR GFP

Plasmid

#184949

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Kp03 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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2C-EGFP-t2a-mCD4

Plasmid

#216213

Purpose

Expresses GFP and mCD4 transmembrane domain under control of the 2C-specific MERVL promoter

Depositor

Michelle Percharde

Article

Xie et al Genes Dev. 2022 Mar 1;36(5-6):331-347. d

Insert

CD4 (Cd4 Mouse)

Tags

GFP-T2A

Expression

Mammalian

Mutation

CD4 transmembrane domain only

Promoter

MERVL

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pB-CAGGS-dCas9

Plasmid

#110823

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pJT302 Ec03 Lenti pEF-attB LSR GFP

Plasmid

#184945

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Ec03 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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