We narrowed to 9,184 results for: Pol;

1. pFastBac HTB His-TEV-BN-rNLRP1-FLAG

   Plasmid

   #217323

   Purpose

   Purification of Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells

   Depositor

   Yifan Wang

   Insert

   NLRP1
   

   Tags

   FLAG and His-TEV

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac HTB His-TEV-LEW-rNLRP1-FLAG

   Plasmid

   #217320

   Purpose

   Purification of Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells

   Depositor

   Yifan Wang

   Insert

   NLRP1
   

   Tags

   FLAG and His-TEV

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57.1-GFP_mmu-miR-16-5p_sponge

   Plasmid

   #132624

   Purpose

   Expresses a TurboGFP transcript with microRNA sponge for mmu-miR-16-5p as 3'UTR

   Depositor

   Constance Ciaudo

   Insert

   stop codon, mmu-miR-16-5p sponge sequence and polyA signal
   

   Use

   Lentiviral; Doxycycline inducible

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. UTP15:PBC028-C01:C245785

   Plasmid

   #183973

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   UTP15 (UTP15 Human)
   

   Use

   Baculovirus expresssion

   Tags

   6xHis-TEV

   Promoter

   Polyhedrin

   Available Since

   May 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. LIPS:SDC101a-A08:C34411

   Plasmid

   #183978

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   LIPS
   

   Use

   Baculovirus expresssion

   Tags

   C-terminal 8xHis tag:EFVEHHHHHHHH and N-termina…

   Promoter

   Polyhedrin

   Available Since

   May 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCDZ2FE_Rev3_1873-2025

   Plasmid

   #105672

   Purpose

   Expresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3 protein

   Depositor

   Thanos Halazonetis

   Insert

   Rev3L (REV3L Human)
   

   Tags

   2X Flag tag

   Expression

   Mammalian

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOpen-ECOligA

   Plasmid

   #165564

   Purpose

   Connects preferentially cohesive double-stranded DNA ends, active on blunt end DNA in the presence of Ficoll or polyethylene glycol. Requires Mg2+ and NAD+. Ligation when blunt end or RNA/ DNA ligation needs to be avoided.

   Depositor

   Drew Endy, Jennifer Molloy, FreeGenes Project

   Insert

   E. coli DNA Ligase
   

   Use

   Synthetic Biology

   Available Since

   Aug. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pTU1-D-spsCD

   Plasmid

   #112788

   Purpose

   Downstream homology arm, SpsC is involved in spore coat polysaccharide synthesis

   Depositor

   Chris French

   Article

   Wicke et al bioRxiv 185108

   Insert

   spsCD
   

   Expression

   Bacterial

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTU1-A-spsCU

   Plasmid

   #112787

   Purpose

   Upstream homology arm, SpsC is involved in spore coat polysaccharide synthesis

   Depositor

   Chris French

   Article

   Wicke et al bioRxiv 185108

   Insert

   spsCU
   

   Expression

   Bacterial

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p404GALS

    Plasmid

    #17435

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    GALS promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    GALS promoter is between SacI and XbaI restrictio…

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac1_FLAG-BPTF

    Plasmid

    #102242

    Purpose

    Expression of human Flag-BPTF using baculovirus from pFastBac1

    Depositor

    Joe Landry

    Insert

    Flag-BPTF (BPTF Human)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    Polyhedron

    Available Since

    Dec. 11, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. 53 pLIB_MBP

    Plasmid

    #160301

    Purpose

    N-term 3C cleavable MBP tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    6xHis and MBP

    Expression

    Insect

    Promoter

    polyhydrin

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOpen-BovDNTT

    Plasmid

    #165580

    Purpose

    Adds dT at the end of PCR blunted DNA fragments/vectors that can then be used in TOPO cloning; Addition of homopolymer tails to the 3' ends of DNA; Labeling the 3' ends of DNA with modified nucleotides (e.g., ddNTP, DIG-dUTP); TUNEL assay (in situ localization of apoptosis); TdT dependent PCR

    Depositor

    Drew Endy, Jennifer Molloy, FreeGenes Project

    Insert

    Bovine DNTT
    

    Use

    Synthetic Biology

    Available Since

    July 28, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. 47 pLIB_GST

    Plasmid

    #160272

    Purpose

    N-term 3C cleavable GST tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    GST

    Expression

    Insect

    Promoter

    polyhydrin

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. 50 pLIB_STREP

    Plasmid

    #160275

    Purpose

    N-term 3C cleavable 2xStrepII tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    2xStrepII

    Expression

    Insect

    Promoter

    polyhydrin

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. p406CYC1

    Plasmid

    #15975

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    CYC1 promoter + polylinker + CYC1 terminator (CYC1 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    CYC1 promoter is between SacI and XbaI restrictio…

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. Stagia3

    Plasmid

    #28177

    Depositor

    Connie Cepko

    Article

    Billings et al PLoS One. 2010 . 5(10):e13739.

    Insert

    Stop TATA
    

    Use

    Chicken expression

    Expression

    Mammalian

    Available Since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRAV23

    Plasmid

    #67715

    Purpose

    Parental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag for native purification

    Depositor

    Robert Batey, Jeffrey Kieft

    Article

    Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

    Insert

    Transcription cassette for 3'-tagged RNAs
    

    Expression

    Bacterial

    Promoter

    T7 RNA polymerase

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

19. YWHAG

    Plasmid

    #39129

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    YWHAG (YWHAG Human)
    

    Tags

    Tev His6

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. LEGO-GM55

    Plasmid

    #217413

    Purpose

    Luciferase expression vector with the minimal GM-CSF promoter

    Depositor

    Peter Cockerill

    Insert

    Luciferase, GFP
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    Human -55 to +28 minimal CSF2 promoter

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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