We narrowed to 9,829 results for: pho;

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His-ATG3-H262A

Plasmid

#202782

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H262A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

H262A

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-T244A

Plasmid

#202781

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-T244A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

T244A

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-Y209A

Plasmid

#202779

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-Y209A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

Y209A

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-Y210A

Plasmid

#202780

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-Y210A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

Y210A

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-K208D

Plasmid

#202785

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-K208D-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

K208D

Available Since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-C264A

Plasmid

#202778

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-C264A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

C264A

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-R265A

Plasmid

#202783

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-R265A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

R265A

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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His-ATG3-H266A

Plasmid

#202784

Purpose

ATG3 mutant overexpression in E.Coli. 6His-TEVsite-ATG3-H266A-stop

Depositor

James Hurley

Article

Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

Insert

ATG3 (ATG3 Human)

Tags

6xHis, TEV

Expression

Bacterial

Mutation

H266A

Available Since

July 19, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-PFKM_sgRNA1

Plasmid

#201610

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

PFKM (PFKM Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-PFKM_sgRNA2

Plasmid

#201611

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

PFKM (PFKM Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-NDUFB5_sgRNA1

Plasmid

#201606

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

NDUFB5 (NDUFB5 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-NDUFB5_sgRNA2

Plasmid

#201607

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

NDUFB5 (NDUFB5 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-PSK2 (K57A)

Plasmid

#197118

Purpose

Expression of kinase-defective MYC-tagged PSK2 (K57A) / TAOK1 (K57A) in mammalian cells

Depositor

Jonathan Morris

Article

Zihni et al J Biol Chem. 2006 Mar 17;281(11):7317-23

Insert

TAOK1 (K57A) (TAOK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Changed K 57 to A

Promoter

SV40

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET-15b(+) Danio rerio PRL (Δprenylation motif)

Plasmid

#166417

Purpose

Express pET-15b(+) Danio rerio PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Danio rerio PRL (ptp4a3a Zebrafish)

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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VHACg/pDONR221

Plasmid

#172094

Purpose

Genomic fragment containing the upstream region and coding region of DET3/VHA-C cloned into pDONR221

Depositor

George Haughn

Article

Shi et al Plant Physiol. 2019 Nov;181(3):901-915.

Insert

VHA-C genomic region including 530bp upstream of the ATG, exons, introns, and no stop codon (DET3 Mustard Weed)

Use

Gateway Cloning

Promoter

530bp upstream of VHA-C ATG

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-EF1α1.1-FLEX-rc [ChromeQ-GFP]

Plasmid

#153542

Purpose

AAV-mediated expression of ChromeQ-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeQ-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

EF1α1.1

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-FLEX-rc [ChromeT-GFP]

Plasmid

#153541

Purpose

AAV-mediated expression of ChromeT-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.

Depositor

Edward Boyden

Article

Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

Insert

ChromeT-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

EF1α1.1

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO FAPP2-GLTPH

Plasmid

#170739

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Ochoa-Lizarralde et al J Biol Chem. 2018 Oct 26;293(43):16709-1

Insert

PLEKHA8-GLTPH (PLEKHA8 Human)

Tags

SUMO

Expression

Bacterial

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO ACD11

Plasmid

#170738

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Simanshu et al Cell Rep. 2014 Jan 30;6(2):388-99. doi:

Insert

acd11 (ACD11 Mustard Weed)

Tags

SUMO

Expression

Bacterial

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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LR (hsp70I: cPLA2-mK2)

Plasmid

#162564

Purpose

Measuring cPLA2 activation in live cells after heat shock.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Inserts

Heat shock protein 70 (HSPA1A Human)
cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Use

Gateway Cloning

Tags

mK2

Promoter

cmlc2

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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