We narrowed to 10,964 results for: T7

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pDONR221-kcnj6

Plasmid

#173133

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Article

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj6 (kcnj6 Zebrafish)

Use

Pcr cloning vector

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (E60amb)-His

Plasmid

#168043

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding E60 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Glu 60 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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bdSUMO-5.3-GFP-HiBit

Plasmid

#171014

Purpose

For bacterial expression of bdSUMO tagged muGFP with 5.3 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-5.3-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pThuAsol

Plasmid

#171675

Purpose

pThuAsol expression in E. coli

Depositor

Wilfred van der Donk

Article

Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

Insert

SUMO-TEV-ThuAsol

Tags

His-tag and SUMO-tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-4

Plasmid

#163130

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-DogTag Loop C

Plasmid

#171781

Purpose

Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp173 and Gly174

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-DogTag Loop C

Tags

His6

Expression

Bacterial

Mutation

DogTag and linkers inserted between residues Asp1…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-SpyTag003 Loop B

Plasmid

#171777

Purpose

Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp102 and Asp103

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-SpyTag003 Loop B

Tags

His6

Expression

Bacterial

Mutation

SpyTag003 and linkers inserted between residues A…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-SpyTag003 Loop C

Plasmid

#171778

Purpose

Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp173 and Gly174

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-SpyTag003 Loop C

Tags

His6

Expression

Bacterial

Mutation

SpyTag003 and linkers inserted between residues A…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (E143amb)-His

Plasmid

#168046

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding E143 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Glu 143 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (S87amb)-His

Plasmid

#168044

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding S87 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Ser 87 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (N116amb)-His

Plasmid

#168045

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding N116 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Asn 116 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (N21amb)-His

Plasmid

#168041

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding N21 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Asn 21 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Fgβ-anticalin-His-ybbr

Plasmid

#168040

Purpose

Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag, Fgβ peptide, and ybbr tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin (I55amb)-His

Plasmid

#168042

Purpose

Expresses anticalin targeting CTLA-4 with the codon encoding I55 changed to amber codon (TAG).

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag

Expression

Bacterial

Mutation

Ile 55 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-3

Plasmid

#163129

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-2

Plasmid

#163128

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding MalE signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Ec-5

Plasmid

#163131

Purpose

pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Article

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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OfCasp3a

Plasmid

#166053

Purpose

a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but has CARD domain and function as an initiator caspase

Depositor

Clay Clark

Article

Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

Insert

Orbicella faveolata caspase-3a

Tags

His tag

Expression

Bacterial

Promoter

T7 lac promoter

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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PaCasp7a

Plasmid

#166051

Purpose

a caspase of Porites astreoides, which has higher sequence similarity with human caspase-7 but has a CARD domain and function as an initiator caspase

Depositor

Clay Clark

Article

Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1

Insert

Porites astreiodes caspase-7a

Tags

His tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRSF-ChPylTMSK

Plasmid

#163915

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK), into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Abdelkader et al J Am Chem Soc. 2021 Jan 5. doi: 10.1021/

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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