We narrowed to 10,431 results for: plasmids 101

1. pXJ-Nluc-_(PLRSKK)-CA1-19

   Plasmid

   #232657

   Purpose

   Nluc-tagged modified CG_ in mammalian cells; lectin-captured luciferase reporter assay

   Depositor

   Chen-Yang Shen

   Article

   Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

   Insert

   CGA (CGA Human)
   

   Use

   Luciferase

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

   Plasmid

   #229823

   Purpose

   A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase-P2A-H2A-mCherry
   

   Expression

   Mammalian

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 945)

   Plasmid

   #229837

   Purpose

   A Piggybac compatible, tet-on, NEO selectable, vector for expressing luciferase followed by a P2A cleavage peptide and H2A fused mCherry.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase
   

   Expression

   Mammalian

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 1154)

   Plasmid

   #229840

   Purpose

   A Piggybac compatible, tet-on, dual reporter encoding luciferase followed by a P2A cleavage peptide and then an H2A fused mCherry for nuclear labeling.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   Luciferase
   

   Expression

   Mammalian

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBiFC-SS-VC155-IRE1-dLD

   Plasmid

   #217751

   Purpose

   Expresses the VC fragment of BiFC-IRE1-dLD

   Depositor

   Christina Chan

   Article

   Cho et al Cell Rep. 2019 Apr 2;27(1):307-320.e5. d

   Insert

   VC155-IRE1-dLD (ERN1 Human)
   

   Tags

   Venus (155-238, A206K)

   Expression

   Mammalian

   Mutation

   deleted amino acids 29-408

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiCRISPRv2_sgALDH7A1

   Plasmid

   #218273

   Purpose

   CRISPR KO plasmid for ALDH7A1 in human cell lines

   Depositor

   Seth Parker

   Article

   Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

   Insert

   Inserted sgRNA targetting ALDH7A1 (ALDH7A1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPBbleo-TGFa-ScNeo

   Plasmid

   #209904

   Purpose

   To monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   TGFa-ScNeo (TGFA Human)
   

   Tags

   GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

   Expression

   Mammalian

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPBbsr2-TGFa-ScNeo

   Plasmid

   #209908

   Purpose

   To monitor the status of TGFα, the plasmid encodes a recombinant TGFα, fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   TGFa-ScNeo (TGFA Human)
   

   Tags

   GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

   Expression

   Mammalian

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBK1300-AAV-EFSNC-dCjCas9-MBD2(176-231)

   Plasmid

   #223145

   Purpose

   Expression of truncated MBD2 with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   MBD2 (MBD2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 176-231

   Promoter

   EF1a

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBK1310-AAV-EFSNC-dSaCas9-MBD2(176-231)

    Plasmid

    #223155

    Purpose

    Expression of truncated MBD2 with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    MBD2 (MBD2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 176-231

    Promoter

    EF1a

    Available Since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. B2M-SDMutation-gRNA

    Plasmid

    #215547

    Purpose

    gRNA targeting B2M to introduce splice donor mutation on the first intron of the locus

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Insert

    B2M (B2M Human)
    

    Use

    CRISPR

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV6-AN-DDK_S172A_M173A RAD23A

    Plasmid

    #201574

    Purpose

    Expresses a variant of human RAD23A containing mutations S172A and M173A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains mutations S172A and M173A

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV6-AN-DDK_V195A RAD23A

    Plasmid

    #201575

    Purpose

    Expresses a variant of human RAD23A containing mutation V195A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains mutation V195A

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV6-AN-DDK_C344A RAD23A

    Plasmid

    #201577

    Purpose

    Expresses a variant of human RAD23A containing mutation C344A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains mutation C344A

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV6-AN-DDK_F354A RAD23A

    Plasmid

    #201578

    Purpose

    Expresses a variant of human RAD23A containing mutation F354A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains mutation F354A

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV6-AN-DDK_C344A_F354A RAD23A

    Plasmid

    #201579

    Purpose

    Expresses a variant of human RAD23A containing mutations C344A and F354A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog A (RAD23A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains mutations C344A and F354A

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV6-AN-DDK_WT RAD23B_delta aa 276-339 (delta XPC-binding domain)

    Plasmid

    #201547

    Purpose

    Expresses a mutant form of human RAD23B that lacks the XPC-binding domain. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

    Insert

    UV excision repair protein RAD23 homolog B (RAD23B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lacks residues 276-339

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. B-HeFSpCas9-A695Q

    Plasmid

    #207368

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A695Q
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, K848A, Q926A, K1003A, R1060A, amino…

    Promoter

    CBh

    Available Since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti HsATP10B_G671R/N865K

    Plasmid

    #204478

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP10B G671R/N865K mutants

    Depositor

    Veerle Baekelandt

    Article

    Wouters et al Biochim Biophys Acta Mol Cell Res. 2024

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Lentiviral

    Mutation

    G671R / N865K

    Promoter

    CMV

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. B-HeFSpCas9-A926Q

    Plasmid

    #207370

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HeFSpCas9-A926Q
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, K848A, K1003A, R1060A, amino…

    Promoter

    CBh

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge