We narrowed to 11,284 results for: VARS

1. TRE3GV-SpdCas9-Dnmt3A/3L-catΔ

   Plasmid

   #222508

   Purpose

   Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant catΔ (C706A and R832E) and hPGK promoter driven mCherry

   Depositor

   Isaac Hilton

   Article

   Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

   Inserts

   S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant catΔ (C706A and R832E) engineered fusion
   mCherry
   

   Use

   Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9; C706A and R832…

   Promoter

   TRE3GV and hPGK

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. IL2-AHDC2-Cherry in pmCherryN1

   Plasmid

   #187291

   Purpose

   Express pmCherry-IL2-AH-deltaC2

   Depositor

   Alpha Yap

   Article

   Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

   Insert

   Scraps interleukin-2 receptor alpha-subunit, anillin (IL2, ANLN Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   AH-deltaC2

   Promoter

   CMV

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONOR-MARINA

   Plasmid

   #223328

   Purpose

   DONOR vector for genomic integration of MARINA voltage sensitive indicator gene from Platisa et al., 2017 (10.1021/acschemneuro.6b00234).

   Depositor

   Jonathan Arias

   Article

   Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do

   Inserts

   MARINA
   KIR2.1 channel Golgi-to-plasma membrane trafficking signal
   

   Use

   CRISPR and Synthetic Biology

   Tags

   mCherry and super-ecliptic pHluorin

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSF_his6_sumo_hRNMT_RAM

   Plasmid

   #213528

   Purpose

   Expresses the human RNMT-RAM complex

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Inserts

   human RNMT (RNMT Human)
   RAM
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSF_his6_sumo_SARS_CoV2_NSP14

   Plasmid

   #213518

   Purpose

   Expresses NSP14 of SARS-CoV-2

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
   

   Tags

   N-terminal His6-sumo

   Expression

   Bacterial

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSF_his6_sumo_SARS_CoV2_NSP14_V290A

   Plasmid

   #213522

   Purpose

   Expresses NSP14 of SARS-CoV-2 with a single point mutation V290A

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   changed V290 to A

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSF_his6_sumo_SARS_CoV2_NSP14_N306K

   Plasmid

   #213523

   Purpose

   Expresses NSP14 of SARS-CoV-2 with a single point mutation N306K

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   SARS-CoV2-NSP14 (ORF1ab SARS-CoV-2)
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   changed N306 to K

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSF_his6_sumo_SARS_CoV2_NSP14_P335S

   Plasmid

   #213524

   Purpose

   Expresses NSP14 of SARS-CoV-2 with a single point mutation P335S

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   changed P335 to S

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSF_his6_sumo_SARS_CoV2_NSP14_P335H

   Plasmid

   #213525

   Purpose

   Expresses NSP14 of SARS-CoV-2 with a single point mutation P335H

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
   

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   changed P335 to H

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRSF_his6_sumo_SARS_CoV2_NSP14_H424R

    Plasmid

    #213526

    Purpose

    Expresses NSP14 of SARS-CoV-2 with a single point mutation H424R

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    changed H424 to R

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. macroH2A2-V192F-GFP (pc5116)

    Plasmid

    #223439

    Purpose

    Mutation of valine 192 to phenylalanine (V192F) in macroH2A2.

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

    Insert

    macroH2A2-F192V (MACROH2A2, H2AFY2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Mutation of valine 192 to phenylalanine (V192F)

    Promoter

    CMV

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGL4.23-hs737

    Plasmid

    #222472

    Purpose

    Luciferase vector containing the hs737 enhancer sequence (reference sequence).

    Depositor

    Tychele Turner

    Article

    Padhi et al Hum Genomics. 2021 Jul 13;15(1):44. doi:

    Insert

    hs737 enhancer (reference sequence) (LOC110120928 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. p-Tet-O-EYA2-Puro

    Plasmid

    #221669

    Purpose

    Expresses EYA2 in mammalian cells

    Depositor

    Vanessa Hall

    Article

    Bergmann et al Front Cell Dev Biol. 2022 Aug 15;10:9765

    Insert

    EYA2 (EYA2 Human)
    

    Use

    Lentiviral

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. p-Tet-O-Foxp1A-Puro

    Plasmid

    #221665

    Purpose

    Expresses Foxp1A in mammalian cells

    Depositor

    Vanessa Hall

    Article

    Bergmann et al Front Cell Dev Biol. 2022 Aug 15;10:9765

    Insert

    Foxp1A (Foxp1 Mouse)
    

    Use

    Lentiviral

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX458-3xHA-FnCas9ABEmax8.17d

    Plasmid

    #201955

    Purpose

    Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    D11A on FnCas9, V82S, V106W, Q154R on mutant TadA

    Promoter

    Cbh

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. str-KDEL_Stx5L(M55V)-SBP-mCitrine

    Plasmid

    #209846

    Purpose

    Synchronize trafficking of Stx5L (M55V) from the ER, for use with FLIM (RUSH system)

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    STX5 (STX5 Human)
    

    Tags

    Streptavidin Binding Protein (SBP) and mCitrine

    Expression

    Mammalian

    Mutation

    Changed Methionine 55 to Valine, causes loss of S…

    Promoter

    CMV

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. sh2g-ZMYND8-human

    Plasmid

    #201406

    Purpose

    Knockdown of Zinc Finger MYND Type-8. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Carney et al Clin Cancer Res. 2023 Jan 24:CCR-22-1896

    Insert

    ZMYND8 (ZMYND8 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. sh4p-ZMYND8-mouse

    Plasmid

    #201405

    Purpose

    Knockdown of Zinc Finger MYND Type-8. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Carney et al Clin Cancer Res. 2023 Jan 24:CCR-22-1896

    Insert

    ZMYND8
    

    Expression

    Mammalian

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1-U6-sgCas9

    Plasmid

    #190903

    Purpose

    AAV-KamiCas9 vector expressing expressing thew GFP reporter gene and sgHTT and sgCas9

    Depositor

    Nicole Déglon

    Article

    Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

    Insert

    GFP, sgHTT and sgCas9 (HTT Human)
    

    Use

    AAV and CRISPR

    Promoter

    EFS and U6

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-HNF1B-FUS-IDR

    Plasmid

    #183240

    Purpose

    Expression of HNF1B fused to a intrinsically disordered regions of the FUS protein

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    HNF1 homeobox B (HNF1B Human)
    

    Tags

    Fusion to an IDR from the FUS protein and FLAG ta…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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