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pHR-CD80-mTFP1

Plasmid

#223605

Purpose

Lentiviral expression of human CD80 with mTFP1 tag in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

mTFP1

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SNAP-PDL1

Plasmid

#223602

Purpose

Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

PD-L1 (CD274 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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BS-TRP1-TetOff(7repeats)-GFP-Pho8

Plasmid

#207011

Purpose

Tet-Off controlled GFP-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.

Depositor

Zhiping Xie

Publication

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

Pho8

Tags

GFP

Expression

Yeast

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1_V5_mNeonGreen_FLAG_C

Plasmid

#218073

Purpose

RESOLUTE cloning plasmid

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Gelová et al J Mol Biol. 2024 Jun 13:168665. doi: 10.

Type

Empty backbone

Use

Lentiviral

Tags

FLAG-mNeonGreen-V5

Available since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3_CDC50A

Plasmid

#203694

Purpose

expresses CDC50A

Depositor

Peter Vangheluwe

Publication

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

TMEM30A (TMEM30A Human)

Expression

Mammalian

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pminiTol2-HuC-NE2m

Plasmid

#208688

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m pan-neuronally in zebrafish

Depositor

Yulong Li

Publication

Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2m

Use

Cloning vector

Promoter

Huc

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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p15a_AcCas12n

Plasmid

#203806

Purpose

p15a-based plasmid containing a rpsL driven AcCas12n cassette and the araBAD-driven lambda-red recombinase cassette. Apramycin resistance.

Depositor

Quanjiang Ji

Publication

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12n

Use

CRISPR

Promoter

rpsl

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSGKP_AcsgRNA

Plasmid

#203807

Purpose

pSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.

Depositor

Quanjiang Ji

Publication

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcgRNA

Use

CRISPR

Promoter

J23119

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-CD79A-YFP

Plasmid

#187004

Purpose

Expression of human CD79A as YFP fusion protein

Depositor

Hermann Eibel

Publication

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

CD79A (CD79A Human)

Use

Lentiviral

Tags

YFP

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMX-SHP1C-ires-hCD8

Plasmid

#183720

Purpose

Expresses the myc-tagged catalytic domains of wild-type SHP-1.

Depositor

Takeshi Tsubata

Publication

Alborzian Deh Sheikh et al J Immunol. 2021 Jun 1;206(11):2544-2551.

Insert

SHP-1

Use

Retroviral

Mutation

catalitic domain alone

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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R

Plasmid

#167170

Purpose

Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cells

Depositor

Susanne Dobler

Publication

Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

Inserts

ATP1A1-R
ATP1B1

Expression

Bacterial and Insect

Promoter

P10 and PH

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-KalTA4-; HE1A:BFP -1

Plasmid

#180011

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Publication

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-KalTA4; HE1A: BFP

Use

Synthetic Biology

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-AviMta3-3xFiBsd

Plasmid

#140962

Purpose

full-length wt Avi-Mta3-3XFLAG-iBsd cDNA w/CAG promoter and Blasticidin resistance, cloned from mouse ES cells

Depositor

Brian Hendrich

Publication

Burgold et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

metastasis associated 3 (Mta3 Synthetic, Mouse)

Tags

3xFLAG, Avi, and TEV

Expression

Mammalian

Promoter

CAG

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pX330_CALR sgRNA / hSpCas9

Plasmid

#172838

Purpose

Mammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Publication

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-B.1.351.v2

Plasmid

#173786

Purpose

Mammalian expression of SARS-CoV-2 Spike protein South African variant version 2

Depositor

Priyamvada Acharya

Publication

Gobeil et al Science. 2021 Jun 24. pii: science.abi62

Insert

Spike (S-GSAS-B.1.351 variant version 2) (S Severe acute respiratory syndrome coronavirus 2)

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-B.1.351.v3

Plasmid

#173787

Purpose

Mammalian expression of SARS-CoV-2 Spike protein South African variant version 3

Depositor

Priyamvada Acharya

Publication

Gobeil et al Science. 2021 Jun 24. pii: science.abi62

Insert

Spike (S-GSAS-B.1.351 variant version 3) (S Severe acute respiratory syndrome coronavirus 2)

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-B.1.351.v6

Plasmid

#173791

Purpose

Mammalian expression of SARS-CoV-2 Spike protein South African variant version 6

Depositor

Priyamvada Acharya

Publication

Gobeil et al Science. 2021 Jun 24. pii: science.abi62

Insert

Spike (S-GSAS-B.1.351 variant version 6) (S Severe acute respiratory syndrome coronavirus 2)

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCW57.1-FLAG-SRSF3_Truncated_Codon.Optimized-Blast

Plasmid

#171952

Purpose

All-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, truncated SRSF3

Depositor

Sujatha Jagannathan

Publication

Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:

Insert

serine and arginine rich splicing factor 3 (SRSF3 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized, truncated at amino acid 114 with…

Promoter

TRE

Available since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-deltaH69/V70

Plasmid

#171744

Purpose

Mammalian expression of SARS-CoV-2 Spike protein S-GSAS-D614G-deltaH69/V70 variant

Depositor

Priyamvada Acharya

Publication

Gobeil et al Science. 2021 Jun 24. pii: science.abi62

Insert

Spike (S-GSAS-D614G-deltaH69/V70 variant) (S Severe acute respiratory syndrome coronavirus 2)

Tags

HRV3C cleavage site, 8X His tag, 2X Strep-Tag II

Expression

Mammalian

Mutation

Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pJV137

Plasmid

#124256

Purpose

Lentiviral vector (pRRL) containing Her2-ITD specific T cell receptor

Depositor

Stanley Riddell

Publication

Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

Insert

T cell receptor (ERBB2 Human)

Use

Lentiviral

Promoter

MSCV

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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