We narrowed to 15,536 results for: LEA

1. pAAV-U6-SpCas9_gRNA(RHO-P23H)-CMV-mTagBFP2_(RAS3613)

   Plasmid

   #228252

   Purpose

   pU6 (human U6) expression of SpCas9 sgRNA targeting RHO P23H mutation and pCMV expression of mTagBFP2

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   SpCas9 P23H sgRNA, mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and U6

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

   Plasmid

   #228253

   Purpose

   pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   U6

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-multi-CRISPR-sgCasp8_#2-puro

   Plasmid

   #231978

   Purpose

   Knockout mouse Casp8

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-multi-CRISPR-sgCasp8_#1-puro

   Plasmid

   #231977

   Purpose

   Knockout mouse Casp8

   Depositor

   Brian Ruffell

   Article

   Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

   Insert

   sgRNA with Cas9 with puromycin resistance (Casp8 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. N-Terminal Split Cas9 with GyrA intein

   Plasmid

   #58693

   Purpose

   Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production

   Depositor

   Gang Bao

   Article

   Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103

   Insert

   Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
   

   Use

   AAV and CRISPR

   Tags

   3xFlag, GyrA Nsplit Intein, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-EF1a-DIO-FLAG-KORD-P2A-ArgiNLS-AausFP1

   Plasmid

   #220612

   Purpose

   Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and a single-cell discriminating version of AausFP1 as a reporter.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   FLAG-KORD-P2A-ArgiNLS-AausFP1
   

   Use

   AAV and Cre/Lox

   Tags

   FLAG; ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1-IDO1-p2a-eGFP

   Plasmid

   #187026

   Purpose

   Expresses IDO1 and GFP (seperately) in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

   Insert

   Indoleamine 2,3-dioxygenase 1 (IDO1 Human)
   

   Use

   Tags

   P2A-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5-FRT/TO-3*Flag-APEX2 N-term SRSF1

   Plasmid

   #187575

   Purpose

   Express FLAG epitope and APEX2-tagged SRSF1 fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   SRSF1 (SRSF1 Human)
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-MCP-SRSF1-V5-mCherry

   Plasmid

   #235088

   Purpose

   SRSF1 mCherry fusion protein with MCP domain

   Depositor

   Mitchell Guttman

   Article

   Bhat et al Nature. 2024 May;629(8014):1165-1173. do

   Insert

   pCAG-MCP-SRSF1-V5-mCherry (SRSF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLHCX-ATF4 mScarlet NLS

    Plasmid

    #115970

    Purpose

    ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I (retroviral vector backbone)

    Depositor

    David Andrews

    Article

    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

    Insert

    activating transcription factor 4 (ATF4 Human, Synthetic)
    

    Use

    Retroviral

    Tags

    HA tag, c-myc NLS, and mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMSCV-FLAG-hIKAROS-IRES-GFP

    Plasmid

    #74046

    Purpose

    retroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    human Ikaros (IKZF1) (IKZF1 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    pMSCV-LTRs

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAVS1-eTLR

    Plasmid

    #214737

    Purpose

    encodes the enhanced traffic light reporter (eTLR) flanked by homology arms for integration into the AAVS1 safe harbor locus

    Depositor

    Gil Westmeyer

    Article

    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

    Insert

    AAVS1-eTLR (PPP1R12C CMV, Anguilla japonica, influenza, Bovine, Chicken, Rabbit, Synthetic, Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pINTO-N3::hTET3

    Plasmid

    #136364

    Purpose

    Expresses epitope-tagged human TET3 WT in mammalian cells

    Depositor

    Roberto Bonasio

    Article

    Beck et al Am J Hum Genet. 2020 Jan 3. pii: S0002-9

    Insert

    TET3 (TET3 Human)
    

    Use

    Tags

    2xStrep-tagII, FLAG, and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDH-CMV-hLamin_A-IRES-copGFP-EF1-puro

    Plasmid

    #132773

    Purpose

    Expression of human LMNA.

    Depositor

    Jan Lammerding

    Article

    Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

    Insert

    Lamin A (LMNA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDule2-3-nitroTyrosine (A7)

    Plasmid

    #174079

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32H H70T D158H I159A L162R

    Promoter

    GlnRS (constitutive)

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5-FRT/TO-3*Flag-APEX2 N-term COILIN

    Plasmid

    #187581

    Purpose

    Express FLAG epitope and APEX2-tagged COILIN fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    COILIN (COIL Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. kiCAP-AAV-MyoAAV1E

    Plasmid

    #224439

    Purpose

    Rep/Cap plasmid for the production of MyoAAV 1E, a muscle-tropic AAV capsid in mice.

    Depositor

    Pardis Sabeti

    Article

    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

    Insert

    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    

    Use

    AAV

    Tags

    Expression

    Mutation

    RGDTMSK insert between amino acids 588 and 589 of…

    Promoter

    p41

    Available since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAGT9054

    Plasmid

    #219430

    Purpose

    Transcriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCS

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    LB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)
    

    Use

    CRISPR; Moclo compatible level 1 module (position…

    Tags

    PapE-4LF2

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SaCas9 variant click editor (CE1) - pCMV-T7-PCV2-nSaCas9-EcKlenow (MLE194)

    Plasmid

    #217801

    Purpose

    Variant CE1 construct with SaCas9, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSaCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSaCas9(N580A); EcKlenow(-exo;D355A/E357A)

    Promoter

    CMV and T7

    Available since

    Sept. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUltra-Hot-PC

    Plasmid

    #184466

    Purpose

    Lentiviral vector for bi-cistronic expression of mCherry and PC (seperated by P2A)

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Pyruvate Carboxylase (PC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge