We narrowed to 9,897 results for: UTY

1. nGR2175-gs10-NLS-VPR

   Plasmid

   #188264

   Purpose

   Plasmid ensures constitutive expression of the N-terminal fragment of split GR2 (aa 1-175), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

   Depositor

   Roman Jerala

   Article

   Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

   Insert

   nGR2175
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZH522

   Plasmid

   #102675

   Purpose

   Tetracycline inducible expression of GFPmut2 with constitutive TetR; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-hDAGLb-V5

   Plasmid

   #87675

   Purpose

   Expresses human DAGLb protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminal

   Depositor

   Pat Doherty

   Insert

   Human Diacylglycerol Lipase Beta (DAGLB Human)
   

   Use

   Tags

   HA and V5 tag

   Expression

   Mammalian

   Mutation

   Hemagglutinin (HA) peptide sequence (YPYDVPDYA) f…

   Promoter

   T7

   Available Since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. BII-ChPtW-PLAGL2

   Plasmid

   #133380

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   PLAGL2 (PLAGL2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQnl_Pcphy23

   Plasmid

   #191354

   Purpose

   Clostridium plasmid expressing NanoLuc reporter from moderate, constitutive promoter

   Depositor

   Andrew Tolonen

   Article

   Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

   Insert

   Pcphy23-NanoLuc
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pcphy23

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZA-GFP-R5

   Plasmid

   #106256

   Purpose

   Constitutively expresses GFP fused with silaffin-R5 silica affinity peptide

   Depositor

   Urartu Seker

   Article

   Olmez et al ACS Omega. 2018 Jan 31;3(1):585-594. doi

   Insert

   Green fluorescent protein
   

   Use

   Tags

   Added silaffin R5 peptide to C terminus of GFP af…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMOD8A-RGR-r10

   Plasmid

   #74370

   Purpose

   This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.

   Depositor

   Eric Klavins

   Article

   Gander et al bioRxiv 041871

   Insert

   RGR-r10
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   April 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWZL-Stat5a1*6

   Plasmid

   #130669

   Purpose

   Retroviral vector for the expression of constitutive Stat5a (hygromycin resistance)

   Depositor

   Gerardo Ferbeyre

   Article

   Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

   Insert

   Stat5a (Stat5a Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   changed Histidine 298 to Arginine and Serine 710 …

   Promoter

   LTR

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRnc-gGFP161

   Plasmid

   #189536

   Purpose

   Expresses E. Coli RNase III under control of pBAD promoter and constitutively expresses gUTR-161-GFP

   Depositor

   Marc Ostermeier

   Article

   Hauk et al CRISPR J. 2022 Dec 9. doi: 10.1089/crisp

   Inserts

   RNase III
   gUTR-161-GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   J23119 and pBAD

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. GLURP-bio-His

    Plasmid

    #50802

    Purpose

    Expresses enzymatically monobiotinylated full length GLURP

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    GLURP
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPotef-LN22*-3xGNLS-cbx

    Plasmid

    #86780

    Purpose

    Expresses lambda N protein fused to triple Gfp coupled to an NLS sequence under the control of the constitutively active Potef promotor.

    Depositor

    Michael Feldbrügge

    Article

    Pohlmann et al Elife. 2015 May 18;4. doi: 10.7554/eLife

    Insert

    LN*
    

    Use

    Ustilago maydis expression

    Tags

    Gfp

    Expression

    Bacterial

    Mutation

    Promoter

    Potef

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Prohibitin-bio-His

    Plasmid

    #50800

    Purpose

    Expresses enzymatically monobiotinylated full length Prohibitin

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    Prohibitin
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. human syndecan 2 EFYA

    Plasmid

    #64971

    Purpose

    Constitutive expression in mammalian cells

    Depositor

    Enric Espel

    Article

    Rovira-Clave et al Cell Signal. 2014 Jul;26(7):1489-99. doi

    Insert

    syndecan 2 (SDC2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted EFYA

    Promoter

    CMV

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

14. human syndecan 2 FAAF

    Plasmid

    #64972

    Purpose

    Constitutive expression in mammalian cells

    Depositor

    Enric Espel

    Article

    Rovira-Clave et al Cell Signal. 2014 Jul;26(7):1489-99. doi

    Insert

    syndecan 2 (SDC2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y179F, S187A, S188A, Y191F

    Promoter

    CMV

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pLenti-FNLSHiFi-P2A-Puro

    Plasmid

    #136902

    Purpose

    Lentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHiFi
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Expression

    Mutation

    R691A

    Promoter

    EF1s

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSELt_S7.A

    Plasmid

    #125835

    Purpose

    Negative selection plasmid for evolution of an amber stop codon suppression tRNA

    Depositor

    Andrew Ellington

    Article

    Maranhao et al ACS Synth Biol. 2017 Jan 20;6(1):108-119

    Insert

    ACP-2xAmb-pheS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pr.trpL*

    Available Since

    May 15, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pPD385 VPR-ZF43(RAAA)

    Plasmid

    #138824

    Purpose

    Constitutive expression of VPR-ZF43(RAAA) under the CMV promoter.

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    VPR-ZF43(RAAA)
    

    Use

    Synthetic Biology

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    R15A, R43A, R71A

    Promoter

    CMV

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMOD8A-iRGR-r11

    Plasmid

    #74369

    Purpose

    This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.

    Depositor

    Eric Klavins

    Article

    Gander et al bioRxiv 041871

    Insert

    RGR-r11
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AHD1

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. IBMc233

    Plasmid

    #161939

    Purpose

    Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PPhlF

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSBbi-TIR1-tTA/Neo

    Plasmid

    #171684

    Purpose

    constitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (G418)

    Depositor

    Randy Poon

    Article

    Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

    Insert

    Neomycin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RPBSA promoter

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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