We narrowed to 17,014 results for: EMB

1. pYFP (GB0053)

   Plasmid

   #68197

   Purpose

   Provides the CDS of the Yellow fluorescente protein as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   YFP coding region
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBGSA FPR1

   Plasmid

   #62600

   Purpose

   Constitutive expression of FPR1 in mammalian cells

   Depositor

   Heini Miettinen

   Article

   Miettinen et al J Immunol. 1997 Oct 15;159(8):4045-54.

   Insert

   FPR1 (FPR1 Human)
   

   Expression

   Mammalian

   Promoter

   SRalpha

   Available Since

   Feb. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

3. phNFL-Q332P

   Plasmid

   #132599

   Purpose

   Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E Q332P mutation

   Depositor

   Anthony Brown

   Article

   Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

   Insert

   Nefl (Nefl Human)
   

   Expression

   Mammalian

   Mutation

   995A>C;Gln332Pro

   Promoter

   CMV

   Available Since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGB3_omega2

   Plasmid

   #68239

   Purpose

   GoldenBraid level 1 Omega plant expression vector

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   empty vector
   

   Use

   Synthetic Biology

   Expression

   Plant

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. PIGAZFN-R2

   Plasmid

   #37800

   Depositor

   Linzhao Cheng

   Article

   Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

   Insert

   PIGA zinc finger R2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFP.R375

   Plasmid

   #138245

   Purpose

   Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(SNL).NrdA-2*.(CNE)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   P(araBAD) and iPC

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTol2-HybridBarcode

   Plasmid

   #174037

   Purpose

   For Tol2 transgenesis of expressed barcode containing SpyCas9, SauCas9, and LbCas12a target sites

   Depositor

   James Gagnon

   Article

   Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

   Insert

   CRISPR target sites
   

   Use

   Tol2 transgenesis

   Available Since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKI-cjVASA-tdTomato

   Plasmid

   #160872

   Purpose

   Marmoset Targeting

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al Stem Cell Res. 2021 Mar 24;53:102308. do

   Insert

   tdTomato
   

   Use

   Marmoset targeting

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMaCTag-P32

   Plasmid

   #120043

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with S-Tag

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   S-Tag
   

   Use

   Pcr template

   Tags

   S-Tag

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMaCTag-Z25

    Plasmid

    #120068

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with YFP-LID

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    YFP-LID
    

    Use

    Pcr template

    Tags

    YFP-LID

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. C455-E01: attB4-stuffer-attB5

    Plasmid

    #162983

    Purpose

    Gateway attB4-attB5 entry clone containing a non-transcribed/translated region for use in generating negative control vectors

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    stuffer fragment for vector controls
    

    Use

    Synthetic Biology

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. C515-E01: attB5r-stuffer-attB1r

    Plasmid

    #162968

    Purpose

    Gateway attB5r-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    stuffer fragment for vector controls
    

    Use

    Synthetic Biology

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. P16ΔS:S10-(MCS)-3xHA

    Plasmid

    #108183

    Purpose

    Binary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Plant

    Promoter

    P16ΔS

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-TGF beta type I receptor (mL45)

    Plasmid

    #34701

    Depositor

    Ying Zhang

    Article

    Yu et al EMBO J. 2002 Jul 15;21(14):3749-59.

    Insert

    TGF beta type I receptor (Tgfbr1 Rat)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    N265A, D267A, N268A

    Promoter

    CMV

    Available Since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA5/FRT/TO_Lyn11-STf

    Plasmid

    #182008

    Purpose

    CMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    Lyn11-STf
    

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. PIGAZFN-L2

    Plasmid

    #37798

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    PIGA zinc finger L2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMaCTag-P08

    Plasmid

    #120019

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mClover3

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mClover3
    

    Use

    Pcr template

    Tags

    mClover3

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-hygro-eGFP-HEC1-9D

    Plasmid

    #114029

    Purpose

    Inducible expression of eGFP-tagged HEC1-9D in FLPin Trex cell lines

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-HEC1 - 9D (NDC80) (NDC80 Human)
    

    Expression

    Mammalian

    Mutation

    Hec1 - 9D

    Available Since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS_FLPER(T)

    Plasmid

    #31131

    Depositor

    Gerhart Ryffel

    Article

    Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

    Insert

    FLP-ER ligand binding domain (ESR1 Human, Budding Yeast)
    

    Expression

    Mammalian

    Mutation

    full length FLP linked to ligand binding domain o…

    Available Since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEG BacMam N term myc eGFP 3C

    Plasmid

    #160682

    Purpose

    Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Tags

    myc eGFP 3C

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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