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lenti-ER-mTagBFP2

Plasmid

#216127

Purpose

Lentiviral vector for expressing mTagBFP2 localizing to the endoplasmic reticulum.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-ProlactinEndoplasmicReticulumSignalSequence-mTagBFP2-KDEL-WPRE

Use

Lentiviral

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pC0046-EF1a-PspCas13b-NES-HIV

Plasmid

#103862

Purpose

Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

PspCas13b

Use

CRISPR and Lentiviral

Tags

3xHA and HIV NES

Expression

Mammalian

Available Since

Nov. 28, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pHIE050 ZF43x0 mKate2 EF1a VP16-ZF43 EF1a EBFP2-P2A-BlastR

Plasmid

#138731

Purpose

Integration Vector, assembled with pHIE042 (TUPV1), pJM466 (TUPV2), pJM452 (TUPV3), pPD613

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x0

Use

Synthetic Biology

Expression

Mammalian

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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myc-BioID2-MCS

Plasmid

#74223

Purpose

To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag

Depositor

Kyle Roux

Article

Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

Myc and humanized A. aeolicus BioID2 with R40G

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV MP-84 mRFP

Plasmid

#206926

Purpose

The micro promoter MP-84 is an AAV specific strong promoter for gene expression in mammalian cells. The small size of the promoter (84 bp) allows delivery of large genes in AAV.

Depositor

Markus Grompe

Article

Chai et al Mol Ther Methods Clin Dev. 2023 May 17;2

Insert

mRFP

Use

AAV

Expression

Mammalian

Promoter

MP-84

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-PGK-puromycin

Plasmid

#51133

Purpose

expresses sgRNA from the U6 promoter.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry_P2AT2A_GFP-nb-KS

Plasmid

#238233

Purpose

For overexpression of mCherry_P2AT2A_GFP-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-GFP-Sec61β

Plasmid

#186597

Purpose

Third generation lentiviral vector expressing GFP-Sec61β

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Sec61β (SEC61B Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Neo DEST (705-1)

Plasmid

#17392

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-COL1A1

Plasmid

#66602

Purpose

Expresses procollagen 1 alpha 1 as a GFP fusion

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2002 Mar 15;115(Pt 6):1149-6

Insert

COL1A1 (COL1A1 Human)

Expression

Mammalian

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pUC57-sgRNA expression vector

Plasmid

#51132

Purpose

For in vitro transcription of sgRNA from the T7 promoter.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

T7

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Flag-WTAP

Plasmid

#53741

Purpose

For expression of WTAP (Wilms tumor 1 associated protein) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Wilms tumor 1 associated protein (WTAP Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry_P2AT2A_GFP-nb-2xKS_FtoG

Plasmid

#238237

Purpose

For overexpression of mCherry_P2AT2A_GFP-nb-2xKS_FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-2xKS_FtoG

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-Mm.cargo(Cre)

Plasmid

#174862

Purpose

Expresses mouse Peg10 cargoRNA encoding Cre recombinase

Depositor

Feng Zhang

Article

Segel et al Science. 2021 Aug 20;373(6557):882-889.

Insert

Cre flanked by MmPeg10 UTRs

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGIPZ-PD-L1-EGFP

Plasmid

#120933

Purpose

Express PD-L1-EGFP fusion protein in mammalian cells

Depositor

Mien-Chie Hung

Article

Yang et al Cell Res. 2018 Aug;28(8):862-864. doi: 1

Insert

PD-L1-EGFP

Expression

Bacterial

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Circular-oc-EMCV-GFP

Plasmid

#226261

Purpose

Production of in vitro circularizable RNA via IVT

Depositor

Prashant Mali

Article

Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

Insert

EGFP

Use

In vitro transcription

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pDualEX-SMN2

Plasmid

#214431

Purpose

Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Insert

SMN2 EXON 7 (SMN2 Human)

Expression

Mammalian

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pT3-EF1a-NICD1

Plasmid

#46047

Depositor

Xin Chen

Article

Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

Insert

Notch1 (C-term) (Notch1 Mouse)

Tags

Myc tag

Expression

Mammalian

Mutation

Cytoplasmic domain initiating at amino acid 1753

Promoter

EF1a

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only

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hprtSAGFP

Plasmid

#41594

Purpose

In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences

Depositor

Maria Jasin

Article

Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.

Insert

SA-GFP reporter

Use

Mouse Targeting; Dsb repair reporter substrate

Expression

Mammalian

Available Since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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cfSGFP2-col4a1

Plasmid

#160317

Purpose

cfSGFP2-tagged collagen4a1 for mammalian expression

Depositor

Ikuo Wada

Article

Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

Insert

COL4A1 (COL4A1 Human)

Tags

cfSGFP2

Expression

Mammalian

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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