We narrowed to 8,563 results for: sars

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pMXS-mitoGshF-blasticidin

Plasmid

#210314

Purpose

Expresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cells

Depositor

Kivanc Birsoy

Article

Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

Insert

Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)

Use

Retroviral

Tags

FLAG peptide, Homo sapiens ACO2 mitochondrial tar…

Mutation

Codon optimized for expression in human cells

Available Since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pTCB-Gal9NFcLALAPGhole6xHis

Plasmid

#237381

Purpose

pTwist CMV BetaGlobin encoding Galectin-9 N terminal carbohydrate binding domain-Fc fusion with LALA P329G hole Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

Galectin-9-silentFc (N-terminal carbohydrate binding domain only) (LGALS9 Human)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pJL1-YPet

Plasmid

#102633

Purpose

In vitro expression of YPet from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

YPet

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE

Plasmid

#162381

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

CAG

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pJL1-mKalama1

Plasmid

#102639

Purpose

In vitro expression of mKalama1 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mKalama1

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE

Plasmid

#162380

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP8s

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

CAG

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE

Plasmid

#162382

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the CAG promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8f

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

Q315L

Promoter

CAG

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-U6-Agrp-(mouse)-MS2gRNA-hSyn-MCP-MSN

Plasmid

#210709

Purpose

This Plasmid express U6 promoter driven SpdCas9 specific gRNA and hSyn promoter driven MCP-MSN transactivation module

Depositor

Isaac Hilton

Article

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

SpdCas9 specific Agrp (mouse) MS2gRNA and MCP-MSN

Use

AAV and CRISPR

Expression

Mammalian

Mutation

N55K in MCP

Promoter

U6

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-THRA-RFP-EGFP

Plasmid

#228537

Purpose

Bichromatic fluorescent reporter of THRA isoform 1 and isoform 2 splicing in mammalian cells

Depositor

Markus Schuelke

Article

Graceffo et al Int J Mol Sci. 2024 Dec 17;25(24):13512.

Insert

Thyroid Hormone Receptor Alpha (THRA Human)

Tags

eGFP and tagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FFSS-BACH1

Plasmid

#232273

Purpose

Expression of N-terminal tagged 2xFLAG-2xSTREP-BACH1 (H. sapiens) in human cell lines

Depositor

Michele Pagano

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

BACH1 (BACH1 Human)

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAC-VIOL

Plasmid

#53087

Purpose

Produces the carotenoid violaxanthin in E. coli.

Depositor

Francis X Cunningham Jr

Article

Neuman et al Plant J. 2014 Apr;78(1):80-93. doi: 10.1

Insert

zep (ABA1 Mustard Weed)

Use

Low copy number bacterial cloning vector

Mutation

Lacks codons for the first 60 N-terminal amino ac…

Promoter

T7

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pBABE-neomycin-PC-MYC

Plasmid

#184550

Purpose

Retroviral vector to express human PC with MYC tag

Depositor

Gerardo Ferbeyre

Article

Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

Insert

Pyruvate Carboxylase (PC Human)

Use

Retroviral

Tags

MYC

Expression

Mammalian

Promoter

LTR

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-FnCas9

Plasmid

#130969

Purpose

3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

Insert

FnCas9

Use

CRISPR

Tags

3xHA-NLS and NLS-T2A-EGFP

Expression

Mammalian

Promoter

Cbh

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-mCherry-FKBP-GEF(ARHGEF1)

Plasmid

#85152

Purpose

acute activation of Rho through rapamycin-induced translocation to membrane-localized FRB

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

Fusion of mCherry, FKBP, and the DH-domain of ARHGEF1 (amino acids 380-630) (ARHGEF1 Human)

Tags

mCherry-FKBP

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pJL1-eBFP2

Plasmid

#102640

Purpose

In vitro expression of eBFP2 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

eBFP2

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-MEKDD

Plasmid

#31202

Purpose

Gateway entry vector containing MEKDD.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

MAPKK1 (MAP2K1 Human)

Use

Gateway donor vector

Mutation

S218D S222D

Available Since

June 24, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HNF1A-WT-Flag-UTR

Plasmid

#183237

Purpose

Expression of FLAG tagged HNF1A

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

HNF1 homeobox A (HNF1A Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pVD1 tRNA-gRNA position E2 (GB2239)

Plasmid

#160561

Purpose

tRNA and scaffold for the assembly of GBoligomers for position [2-3] of a polycistronic tRNA-gRNA

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

tRNA-gRNA position E2 (Multiplexing Edit)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHOT-P2Y2R

Plasmid

#159108

Purpose

entiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high affinity G-protein coupled receptor that mobilizes Ca2+ from intracellular stores upon binding extracellular ATP.48,49 By using the Gibson

Depositor

Fabio Mammano

Article

Mazzarda et al Lab Chip. 2020 Jul 23. doi: 10.1039/d0lc

Insert

P2Y2R

Use

Lentiviral

Promoter

UBC

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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Gal4-HA-HIF1α(530-652)

Plasmid

#24887

Depositor

William Kaelin

Article

Ivan et al Science. 2001 Apr 20. 292(5516):464-8.

Insert

hypoxia inducible factor1alpha (HIF1A Human)

Tags

Gal4dbd and HA-tag

Expression

Mammalian

Mutation

partial HIF1alpha cDNA (encoding aa 530-652)fused…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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