We narrowed to 24,348 results for: Sis

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3xEGFP-mMeikin (E156R, R159E)

Plasmid

#174710

Purpose

Mammalian expression of mouse Meikin with Separase-resistant mutations

Depositor

Iain Cheeseman

Article

Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

Insert

Meikin (Meikin Mouse)

Tags

3xEGFP

Expression

Mammalian

Mutation

E156R, R159E

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pNOC-CRISPR-35S

Plasmid

#99861

Purpose

Expresses Cas9-Nlux and sgRNA. Contains CEN/ARS and 35 terminator. No selection marker.

Depositor

Eva Farre

Article

Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

Type

Empty backbone

Use

Algae, nannochloropsis expression

Tags

Cas9-Nlux

Promoter

Ribi promoter

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pOVC1_AtPHYB-S_X

Plasmid

#131183

Purpose

Expresses AtPHYB-S (Arabidopsis thaliana) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

AtPHYB-S

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pPAtUbq10 (GB0223)

Plasmid

#68174

Purpose

Provides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtUbq10 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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SEPLuc-IRES-Antares

Plasmid

#141085

Purpose

precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEPLuc-EMCV IRES-Antares

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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pPAct2noUTR (GB0192)

Plasmid

#68177

Purpose

Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtAct2 promoter (no UTR)

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTAtUbq3 (GB0219)

Plasmid

#68192

Purpose

Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtUbq3 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pHyg-AID(31-114)-9myc

Plasmid

#99517

Purpose

Truncated C-terminal AID-9myc degron cassette with HygR marker for S. cerevisiae

Depositor

Helle Ulrich

Article

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(31-116) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR-IAA17_no stop

Plasmid

#83855

Purpose

Gateway donor vector for IAA17 with no stop codon

Depositor

Jennifer Nemhauser

Article

Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

Insert

IAA17 (AXR3 Mustard Weed)

Use

Gateway Cloning

Mutation

no stop codon

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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JW616

Plasmid

#64805

Purpose

CUC2 expression in yeast

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC2 (CUC2 Mustard Weed)

Tags

GAL4 Activation Domain, HA, and SV40 NLS

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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