We narrowed to 8,596 results for: Set

1. pSCL452

   Plasmid

   #219502

   Purpose

   Golden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t

   Depositor

   Seth Shipman

   Article

   Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

   Insert

   GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t
   

   Expression

   Yeast

   Promoter

   GAL7

   Available Since

   May 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pILGFP4QE2Crimson

   Plasmid

   #218280

   Purpose

   Introduction of a bicistronic expression cassette of yEGFP and E2Crimson under the control of the SkGAL2 promoter

   Depositor

   Claudia Vickers

   Article

   Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

   Insert

   pURA3>KlURA3>tKlURA3-pSkGAL2>yEGFP>ERBV1.2A>E2Crimson>tURA3
   

   Expression

   Yeast

   Available Since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AA363

   Plasmid

   #215955

   Purpose

   Fragmid fragment: (guide cassette) guide expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   dU6:3_v1; BsmBI_v14; BsmBI_v1; trRNA_v1 [Sp]
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AA303

   Plasmid

   #216071

   Purpose

   Fragmid fragment: (guide cassette) guide expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; BsmBI_v0; BsmBI_v6; trRNA_v1 [Nme2]; terminator_v1
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AA022

   Plasmid

   #215930

   Purpose

   Fragmid fragment: (guide cassette) 2xPP7 & 2xMS2 in trRNA

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; sgCD4_v3 [Sp]; trRNA_v14 [Sp]; CS2
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AA016

   Plasmid

   #215927

   Purpose

   Fragmid fragment: (guide cassette) guide expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v3
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AA017

   Plasmid

   #215928

   Purpose

   Fragmid fragment: (guide cassette) 2xPP7 & 2xMS2 in trRNA

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v14 [Sp]; CS2
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AA403

   Plasmid

   #215960

   Purpose

   Fragmid fragment: (guide cassette) guide expression

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; DR_v0 [Cas7-11]; BsmBI_v16; BsmBI_v12
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AA151

   Plasmid

   #215938

   Purpose

   Fragmid fragment: (guide cassette) for dual expression sgRNA

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6_v1; BsmBI_v0; BsmBI_v4; rev{H1_v1}
   

   Use

   CRISPR; Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AA400

    Plasmid

    #215958

    Purpose

    Fragmid fragment: (guide cassette) guide expression

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; DR_v1 [Rfx]; BsmBI_v1; BsmBI_v12
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AA401

    Plasmid

    #215959

    Purpose

    Fragmid fragment: (guide cassette) guide expression

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6_v1; DR_v1 [Dj]; BsmBI_v1; BsmBI_v12
    

    Use

    CRISPR; Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMDC32B-AtTAS1c-D2-NbSu-2-AtMIR173a

    Plasmid

    #213401

    Purpose

    Plant expression vector (2x35S) for expressing a syn-tasiRNA against Nicotiana benthamiana SULFUR gene from AtTAS1c precursor

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:

    Insert

    Arabidopsis TAS1c with a syn-tasiRNA sequence at D2 for silencing N. benthamiana SULFUR gene. MIR173 cassette.
    

    Expression

    Plant

    Promoter

    2x35S

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV.5’eGFP/sgLMNA

    Plasmid

    #170546

    Purpose

    Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP without homology arms

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    sgRNA targeting 5'- end of LMNA
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV.5’eGFP/sgVIM

    Plasmid

    #170548

    Purpose

    Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP without homology arms

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    sgRNA targeting 5'- end of VIM
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA

    Plasmid

    #174897

    Purpose

    ochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sf GFP
    

    Expression

    Mammalian

    Mutation

    150TAA in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGA

    Plasmid

    #174898

    Purpose

    opal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TGA in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGA-red-mini

    Plasmid

    #196338

    Purpose

    Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology; Golden gate assembly backbone

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGA-red-maxi

    Plasmid

    #196337

    Purpose

    Easy-MISE toolkit Level 1 destination plasmid for cassette cloning with BsaI-based Golden Gate Assembly; allowing fast postitive clones test with mRFP1 fluorescent protein-based red/white screening

    Depositor

    Paola Branduardi

    Article

    Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

    Type

    Empty backbone

    Use

    Synthetic Biology; Golden gate assembly backbone

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSCL.178

    Plasmid

    #184999

    Purpose

    Express -Eco1 FANCF editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: FANCF targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    FANCF donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSCL.176

    Plasmid

    #184997

    Purpose

    Express -Eco1 RNF2 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RNF2 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    RNF2 donor, a1/a2 length extended for 27 bp

    Promoter

    H1

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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