We narrowed to 7,762 results for: loxP
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Plasmid#204725PurposeConstitutive CRISPRi by Zim3-dCas9InsertZim3-dCas9
ExpressionMammalianAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C1
Plasmid#189618PurposeCloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertMitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINSPECT:NEAT1-total_SigP:NLuc
Plasmid#197267PurposeDonor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.DepositorInsertNEAT1-total homology arms
UseCRISPR, Luciferase, Synthetic Biology, and Unspec…ExpressionMammalianAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWPI-UPP2-3xFLAG
Plasmid#201645PurposeOver-expression of 3xFLAG-tagged human UPP2DepositorAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C4
Plasmid#189621PurposeCloned Reduced Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertReduced Mitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionExpressionBacterial and YeastMutationRepeat region removed, and minor additional mutat…Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTP-PCR C2
Plasmid#189619PurposeCloned Mitochondrial Genome of the diatom Thalassiosira pseudonana in yeast and bacteriaDepositorInsertMitochondrial Genome of Thalassiosira pseudonana
UseSynthetic Biology; Diatom expressionExpressionBacterial and YeastMutationMinor additional mutations described in manuscriptAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPT-PCR C1
Plasmid#141248PurposePCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 1DepositorPublicationInsertReduced Mitochondrial Genome of Phaeodactylum tricornutum
UseSynthetic Biology; Diatom expressionExpressionBacterial and YeastMutationRepeat region removed, additional minor mutations…Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pInducer 21-3xFlag-hMEFV-F479L
Plasmid#195477PurposepInducer21 plasmid containing the human MEFV gene with the F479L mutation (associated with Familial Mediterranean Fever) with a 3xFlag tag at the N-terminusDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMammalianMutationchanged phenylalanine 479 to leucineAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pInducer 21-3xFlag-hMEFV-ΔPYD
Plasmid#195478PurposepInducer21 plasmid containing the human MEFV gene with a deletion of residues 1 - 92 (the resulting pyrin protein lacks the PYD domain) with a 3xFlag tag at the N-terminusDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMammalianMutationdeletion of residues 1-92 of pyrin (the PYD domai…Available sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pInducer21-3xFlag-hMEFV-Q426R
Plasmid#195475PurposepInducer21 plasmid containing the human MEFV gene with the Q426R mutation (associated with Familial Mediterranean Fever) with a 3xFlag tag at the N-terminusDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMammalianMutationchanged glutamine 426 to arginineAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pInducer 21-3xFlag-hMEFV-ΔPLD
Plasmid#195479PurposepInducer21 plasmid containing the human MEFV gene with a deletion of residues 88 - 310 (the resulting pyrin protein lacks the Phosphorylated Linker Domain domain) with a 3xFlag tag at the N-terminusDepositorInsertMEFV (MEFV Human)
UseLentiviralTags3xFlagExpressionMammalianMutationdeletion of residues 88 - 310 (the resulting pyri…Available sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV/EF1a::DIO-iLMO2
Plasmid#129482PurposeAAV2 transfer plasmid for Cre-dependent expression of iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemicalDepositorInsertiLMO2
UseAAVExpressionMammalianPromoterEF1aAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgT-REX17_EF1a-Puro-T2A-BFP
Plasmid#195501PurposeLentiviral BFP expression vector bearing a sgRNA targeting the promoter of human T-REX17DepositorInsertsgRNA
UseCRISPR and LentiviralTagsBFP, Puro, and T2AExpressionMammalianAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3a- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192236Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3aDepositorInserteIF3a-knockin-homology arm-mAID-mCherry2-Hygro (EIF3A Human)
ExpressionMammalianAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3k- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192244Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3kDepositorInserteIF3k-knockin-homology arm-mAID-mCherry2-Hygro (EIF3K Human)
ExpressionMammalianAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
eIF3b- pMK293 (mAID-mCherry2-Hygro) plasmid
Plasmid#192238Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3bDepositorInserteIF3b-knockin-homology arm-mAID-mCherry2-Hygro (EIF3B Human)
ExpressionMammalianAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCXLE-mCherry-t2a-SOX2AV-p2a-KLF4
Plasmid#193295Purposeepisomal vector expressing mCherry + human SOX2A61V + KLF4DepositorExpressionMammalianMutationSOX2A61V is a highly cooperative point mutant of …PromoterCAGAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS-dsarm(E)::flag
Plasmid#187871PurposeGal4/UAS expression of dSarm(isoE)::FLAGDepositorInsertdSarm isoform E (Sarm Fly)
TagsFLAGAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-GG-hUbC-EBPF2-gRNA-RBMS3
Plasmid#185557PurposeLentiviral backbone with EBFP2 reporter and gRNA targeting RBMS3DepositorInsertRBMS3 gRNAs
UseCRISPR and LentiviralExpressionMammalianAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINF Ta-sro1 PARP
Plasmid#192549PurposeE. coli expression vector for Triticum aestivum sro1 PARP domain (amino acids 246-434)DepositorInsertSIMILAR TO RCD ONE 1 (SRO1) PARP domain, cultivar Shanrong No. 3 (SR3)
TagsHis6, 3C protease cleavage siteExpressionBacterial, Insect, and Mamm…PromoterT7 promoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only