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We narrowed to 2,467 results for: puc19

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Showing: 371 - 380 of 2467 results

  1. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid
    #223137
    Purpose
    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223137-plasmid-map-sequence-id-447707

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  3. pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)

    Plasmid
    #133792
    Purpose
    U6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    St3Cas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Muller et al. Molecular T…
    Promoter
    U6
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133792-plasmid-map-sequence-id-268589

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  4. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid
    #215769
    Purpose
    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus
    Depositor
    Dimitrios Wagner
    Article
    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:
    Inserts
    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Bovine, Mouse, Human)
    TRAC-3' homology arm (TRAC Human)
    Expression
    Bacterial
    Mutation
    Cas12a PAM mutated
    Available Since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    215769-plasmid-map-sequence-id-435524

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  8. pUC19 (10/20nt-gap) (NN1)

    Plasmid
    #257527
    Purpose
    To create a 10nt single-stranded gap by nicking with (Nb.BbvCI & Nb.BsmI) or (Nb.BsmI & Nt.BspQI) or a 20nt single-stranded gap by nicking with Nb.BbvCI, Nb.BsmI, and Nt.BspQI
    Depositor
    Michael O'Donnell
    Insert
    Nb.BbvCI, Nb.BsmI, and Nt.BspQI nicking sites
    Use
    Not for gene expression. a dna template for bioch…
    Available Since
    June 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    257527-plasmid-map-sequence-id-512365

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  9. pUC19-KanMX-msGFP2-YPT6

    Plasmid
    #255054
    Purpose
    Integrate an N-terminally tagged msGFP2-Ypt6 sequence at the YPT6 locus. This is a second copy integration.
    Depositor
    Benjamin Glick
    Insert
    msGFP2-Ypt6
    Expression
    Yeast
    Available Since
    June 1, 2026
    Availability
    Academic Institutions and Nonprofits only
    255054-plasmid-map-sequence-id-511758

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Showing: 371 - 380 of 2467 results