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pET28a-HypD

Plasmid

#110288

Purpose

Expresses t-4-hydroxy-L-proline dehydratase in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

t-4-Hydroxy-L-proline Dehydratase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-PDAE

Plasmid

#110180

Purpose

Expresses propanediol dehydratase activating enzyme in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase Activating Enzyme

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-PD-H166Q

Plasmid

#110179

Purpose

Expresses propanediol dehydratase mutant H166Q in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Tags

His6 and S-tag

Expression

Bacterial

Mutation

H166Q mutant

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-PD-E440Q

Plasmid

#110178

Purpose

Expresses propanediol dehydratase mutant E440Q in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Tags

His6 and S-tag

Expression

Bacterial

Mutation

E440Q mutant

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-PD-C438S

Plasmid

#110177

Purpose

Expresses propanediol dehydratase mutant C438S in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Tags

His6 and S-tag

Expression

Bacterial

Mutation

C438S mutant

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-PD-G817A

Plasmid

#110174

Purpose

Expresses propanediol dehydratase mutant G817A in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Tags

His6 and S-tag

Expression

Bacterial

Mutation

G817A mutant

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-PD

Plasmid

#110168

Purpose

Expresses propanediol dehydratase in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

Propanediol Dehydratase

Tags

His6 and S-tag

Expression

Bacterial

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS Exon

Plasmid

#110119

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicing

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

dati (dati Fly)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati Exons 1-3

Plasmid

#110113

Purpose

Drosophila dati minigene plasmid that expresses both a linear RNA and a circular RNA

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

dati (dati Fly)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU74.1-2.2

Plasmid

#110689

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Tags

flag

Expression

Yeast

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRS315Nab3-11

Plasmid

#110534

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

Insert

NAB3 (NAB3 Budding Yeast)

Expression

Yeast

Mutation

two missense mutations creating Nab3-11

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU70.1-2.2

Plasmid

#110682

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Tags

flag

Expression

Yeast

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU70.2-2.2

Plasmid

#110683

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4

Expression

Yeast

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCHKU33.2-2.2

Plasmid

#110667

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6

Expression

Yeast

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU72.1-2.2

Plasmid

#110686

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Tags

flag

Expression

Yeast

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCHKU32.2-2.2

Plasmid

#110665

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4

Expression

Yeast

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU72.2-2.2

Plasmid

#110687

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3

Expression

Yeast

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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St1Cas9_LMD-9_sgRNA_pUC19

Plasmid

#110627

Purpose

U6-driven sgRNA expression plasmid for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9).

Depositor

Yannick Doyon

Article

Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

Insert

Empty St1Cas9 LMD-9 sgRNA backbone

Use

CRISPR and Synthetic Biology

Tags

None

Expression

Mammalian

Promoter

hU6

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS circfirefly_1100

Plasmid

#110117

Purpose

Expresses a 1100-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS circfirefly_900

Plasmid

#110116

Purpose

Expresses a 900-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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