Addgene

We narrowed to 1,561 results for: CAST

Showing: 741 - 760 of 1561 results

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pPTctp (GB0083)

Plasmid

#68169

Purpose

Provides the S. Lycopersicum translationally-controlled tumor protein homolog promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlTCTP promoter

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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p5'UTRAct2 (GB0193)

Plasmid

#68178

Purpose

Provides the 5'-untranslated region of the Arabidopsis thaliana actin2 gene as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

5'UTR AtAct2

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pEGB 1alfa1R Tnos:Hygromycin:PNos (GB0235)

Plasmid

#68213

Purpose

Transcriptional unit for Hygromycin resistance gene plant expression

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Hyg

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pDGB1omega1_SF (GB0158)

Plasmid

#68242

Purpose

Twister plasmid to swap inserts from an omega2 or omega2R vector to any alpha level vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Solanum lycopersicum intergenic region

Use

Synthetic Biology; Unspecified

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pMyb12 (GB0075)

Plasmid

#68199

Purpose

Provides the CDS of theSolanum lycopersicum Myb12 transcription factor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Myb12 coding region

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pPAt2S3 (GB0029)

Plasmid

#68162

Purpose

Provides the Arabidopsis thaliana Seed-specific promoter 2S3 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

At2S3 promoter

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pUPD_Mini35s(-60,UTR) (GB0050)

Plasmid

#68176

Purpose

Provides the CaMV Mini35S Promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

CaMV Mini35S promoter

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pIS57 PGM2 3'UTR mut

Plasmid

#14492

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

PGM2 3'UTR mutant (PGM2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CATTCC’s were changed to AAGTAC's

Promoter

Available since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pIS56 NETO2 3'UTR mut

Plasmid

#14496

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

NETO2 3'UTR mutant (NETO2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CATTCC’s were changed to AAGTAC's

Promoter

Available since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pIS55 POGK 3'UTR mut

Plasmid

#14498

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

POGK 3'UTR mutant (POGK Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

First, fourth, and fifth occurrences of CATTCC’s …

Promoter

Available since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
pIS53 SERP1 3'UTR mut

Plasmid

#14500

Depositor

David Bartel

Article

Lim et al Nature. 2005 Feb 17. 433(7027):769-73.

Insert

SERP1 3'UTR mutant (SERP1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

CATTCC’s were changed to AAGTAC's

Promoter

Available since

March 7, 2007

Availability

Academic Institutions and Nonprofits only
AAV-KP1

Plasmid

#206504

Purpose

AAV packaging vector containing AAV2 rep and KP1 cap and partial AAV2 p5 promoter

Depositor

Mark Kay

Article

Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

Insert

KP1 capsid

Use

AAV

Tags

Expression

Mutation

Promoter

p5

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-EGFPKASH

Plasmid

#124882

Purpose

labelling of neuronal nuclear envelope with EGFP

Depositor

Vassilis Pachnis

Article

Obata et al Nature. 2020 Feb;578(7794):284-289. doi:

Insert

EGFP

Use

AAV

Tags

KASH

Expression

Mutation

Promoter

CaMKII

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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Cas12aUltra_5XNLS

Plasmid

#218775

Purpose

His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS

Depositor

Ross Wilson

Article

Sahu et al bioRxiv 2024.07.14.603391

Insert

5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a

Use

CRISPR

Tags

6XHis (C terminal), HA, Nucleoplasmin, SV40, and …

Expression

Bacterial

Mutation

Nucleotides mutations were made to encode for arg…

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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CMV BARK D110A p2A mCherry

Plasmid

#117690

Purpose

CMV-iBARK(D110A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs D110A peptide

Use

Tags

HA / FLAG

Expression

Mammalian

Mutation

D110A

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV9-null

Plasmid

#200182

Purpose

Expresses AAV2 Rep and AAV9 Cap with nonsense mutation at VR8 site

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV9 Cap w/ nonsense mutation at VR8

Use

AAV

Tags

Expression

Mammalian

Mutation

Replaced amino acids 586-592 in Cap with nonsense…

Promoter

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Aqp4-BioID2-BioID2-HA

Plasmid

#176742

Purpose

Expresses Aquaporin-4 fused with BioID2 at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Aqp4-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Mutation

Promoter

gfaABC1D

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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PZac2.1 hSyn1-HA-BioID2-SAPAP3

Plasmid

#176857

Purpose

Expresses SAPAP3 protein fused with BioID2 at the N-terminus in neurons

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

HA-BioID2-SAPAP3

Use

AAV

Tags

HA

Expression

Bacterial

Mutation

Promoter

hSyn1

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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GfaABC1D BARK p2A mCherry

Plasmid

#117691

Purpose

GfaABC1D-iBARK-p2A-mCherry: Viral expression vector for astrocyte Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs peptide

Use

AAV

Tags

HA / FLAG

Expression

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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HDAC8 Flag

Plasmid

#13825

Depositor

Eric Verdin

Article

Waltregny et al FASEB J. 2005 Jun . 19(8):966-8.

Insert

HDAC8 (HDAC8 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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