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sgiRFP3

Plasmid

#113974

Purpose

Single short guide RNA targeting CGTGATGTTGTACCGCTTC in iRFP670 sequence

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

iRFP670 sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMAL-C2X -MTalpha-FliG

Plasmid

#154361

Purpose

Bacterial expression of Mtalpha

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Mtalpha

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMAL-C2X -MTn-FliG

Plasmid

#154360

Purpose

Bacterial expression of MTn

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

MTn

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMAL-C2X dAmp/Kana+-AFP

Plasmid

#154359

Purpose

Bacterial expression of AFP

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

AFP

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMAL-C2X -2AFP-FliG

Plasmid

#154362

Purpose

Bacterial expression of 2AFP

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

2AFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMAL-C2X dAmp/Kana+-2AFP

Plasmid

#154358

Purpose

Bacterial expression of 2AFP

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

2AFP

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSP569

Plasmid

#139499

Purpose

Repair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiae

Depositor

Serge Pelet

Article

Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

Insert

pSTL1 24xPP7sl STL1 100 - 600

Use

Tags

Expression

Yeast

Mutation

WT

Promoter

pSTL1

Available since

June 12, 2020

Availability

Academic Institutions and Nonprofits only

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sg1

Plasmid

#113966

Purpose

Single short guide RNA targeting GTATAGCATACATTATACG

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg1

Use

Tags

Expression

Mutation

Promoter

U6

Available since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTrcHis-7D12Asp73TAG

Plasmid

#125266

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at Asp73 for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

7D12 Asp73TAG

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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AIRAP 5: AIRAP(1-50).GST,V1.pcDNA3

Plasmid

#21795

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

AIRAP (Zfand2a Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

amino acids 1-50

Promoter

Available since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B R398,399Q IRES GFP

Plasmid

#133819

Purpose

Encodes the C2B domain of synaptotagmin 1 with mutations R398,399Q for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Expression

Mutation

R398,399Q

Promoter

hSyn (human synapsin I promoter)

Available since

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B K326,327E IRES GFP

Plasmid

#133820

Purpose

Encodes the C2B domain of synaptotagmin 1 with mutations K326,327E for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Expression

Mutation

K326,327E

Promoter

hSyn (human synapsin I promoter)

Available since

Availability

Academic Institutions and Nonprofits only

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FRB-mCherry-Sec61β

Plasmid

#186574

Purpose

Expression of FRB-mCherry-tagged ER protein Sec61beta in mammalian cells

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

SEC61B (SEC61B Human)

Use

Tags

FRB-mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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PB-fLuc=tdT

Plasmid

#120870

Purpose

Piggybac transposon plasmid for live animal optical imaging

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

pcDNA3-fLuc=tdT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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human oct4-GFP

Plasmid

#21153

Depositor

Rudolf Jaenisch

Article

Green et al Nano Lett. 2008 Oct . 8(10):3126-30.

Insert

hOCT4-GFP (POU5F1 Human)

Use

Human targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Cep83

Plasmid

#128874

Purpose

For mammalian expression of mouse Cep83

Depositor

Aimin Liu

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

Insert

Cep83 (Ccdc41 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pJRH-1362 scFv entry plasmid

Plasmid

#201912

Purpose

Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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σ3-mCherry

Plasmid

#186580

Purpose

Expression of adaptor protein 3 (AP3) sigma subunit AP3S1 tagged with mCherry

Depositor

Stephen Royle

Article

Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

Insert

AP3S1 (AP3S1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pJRH-1180 U6-reci Gag-pol v2

Plasmid

#201915

Purpose

Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-pol

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pJRH-1179 U6-reci Gag-Cas9 v2

Plasmid

#201914

Purpose

Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-Cas9 v2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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