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pCDNA3-p18-mCherry-LOVPLD

Plasmid

#219560

Purpose

lysosome-targeted LOVPLD

Depositor

Jeremy Baskin

Article

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

p18-mCherry-LOVPLD

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pBetaActin-dCer3-C2-FRB

Plasmid

#191327

Purpose

Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-C2-dCer3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FRB-dCer3-CAAX

Plasmid

#191326

Purpose

Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-dCer-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-mEos3.2

Plasmid

#191320

Purpose

Expresses fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mEos3.2 (Tubb2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-Dronpa

Plasmid

#191321

Purpose

Expresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV light

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-Dronpa (Tubb2a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJEP229-Lenti-TRE3G-4xMyc-GluN2B-WPRE-pA

Plasmid

#164794

Purpose

pLenti vector backbone designed to express 4XMyc Tagged-GluN2B from a TRE3G promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

4xMyc

Expression

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p09008_AF1-4+GFP5-7

Plasmid

#173715

Purpose

Plasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.

Depositor

Johannes Kabisch

Article

Zibulski et al Front Bioeng Biotechnol. 2022 Apr 1;10:8

Inserts

hybrid GFP
mKate2

Use

E. coli cell free protein synthesis extracts

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP231-Lenti-TRE3G-Flag-GluN2B(E1479Q)-WPRE-pA

Plasmid

#164796

Purpose

pLenti vector backbone designed to express Flag Tagged-GluN2B(E1479Q) from a TRE3G promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP201-Lenti-TRE3G-Flag-GluN2B-WPRE-pA

Plasmid

#164775

Purpose

pLenti vector backbone designed to express Flag-GluN2B from a TRE3G promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP226-Lenti-CMV-Flag-GluN2B-WRPE-pA

Plasmid

#84275

Purpose

pLenti vector backbone designed to express Flag-GluN2B from a CMV promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

CMV

Available since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP225-Lenti-CMV-Flag-GluN2A-WPRE-pA

Plasmid

#84274

Purpose

pLenti vector backbone designed to express Flag-GluN2A from a CMV promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Flag-GluN2A

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

CMV

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP200-Lenti-TRE3G-Flag-GluN2A-WPRE-pA

Plasmid

#164724

Purpose

pLenti vector backbone designed to express Flag-GluN2A from a TRE3G promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2A

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP202-Lenti-TRE3G-GFPGluN2A-WPRE-pA

Plasmid

#164776

Purpose

pLenti vector backbone designed to express GFP-GluN2A from a TRE3G promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2A

Use

Lentiviral

Tags

GFP

Expression

Mutation

Promoter

1.3αCaMKII

Available since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP214-Lenti-0.5Synapsin P-GFP- WPRE-pA

Plasmid

#62929

Purpose

pLenti vector backbone designed to express GFP from a 0.5Synapsin promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

0.5synapsin and 1.3αCaMKII

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP215-Lenti-0.4CaMKIIa P-GFP-WPRE-pA

Plasmid

#62927

Purpose

pLenti vector backbone designed to express GFP from a 0.4CaMKIIa promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

0.4αCaMKII and 1.3αCaMKII

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP213-Lenti-1.1Synapsin P-GFP-WPRE-pA

Plasmid

#62930

Purpose

pLenti vector backbone designed to express GFP from a 1.1Synapsin promoter .

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Green Fluorescent protein (GFP)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

1.1Synapsin and 1.3αCaMKII

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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RP51A-No5'ss-NoBS-NoPseudoBS

Plasmid

#100987

Purpose

RP51A with T7 promoter in HindIII/EcoRI sites of pUC18. No 5'ss, No BS, No pseudo BS

Depositor

Aaron Hoskins

Article

Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

Insert

RP51A (RPS17A Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

5'ss mut to C/ATACCT BS mut to GTTAGTG, pseu…

Promoter

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-TREX1 g3 Cas9-T2A-mCherry

Plasmid

#164252

Purpose

Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgTREX1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSTAR2

Plasmid

#166940

Purpose

STAR-based multi-level gene expression controller (MLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Circuit expressing GFP- Small transcription activating RNAs

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PTAC

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pSTAR

Plasmid

#166939

Purpose

STAR-based multi-level gene expression controller (MLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Genetic circuit expressing GFP- Small Transcription activating RNA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PTAC

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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