We narrowed to 1,020 results for: TSK

1. pAAV-TRE-DIO-GCaMP6s

   Plasmid

   #183809

   Purpose

   his plasmid is for use with neuronal cell-type selective activity tagging. The GCaMP6 expression requires both neural activity and Cre recombinase.

   Depositor

   William Wisden

   Article

   Yu et al Science. 2022 Jul;377(6601):63-72. doi:

   Insert

   AAV transgene TetO promoter-DIO/flex-GCaMP6
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNH605-HSE-YFP

   Plasmid

   #172131

   Purpose

   Fluorescent reporter for Hsf1 activity

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   4xHSE-YFP
   

   Use

   Vector for genomic integration into the yeast gen…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CYC1 core promotor with 4 tandem repeats of the H…

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CaMKII-iGECI-NES

   Plasmid

   #160424

   Purpose

   AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI with a nuclear export signal sequence

   Depositor

   Vladislav Verkhusha

   Article

   Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

   Insert

   iGECI-NES
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Hela kinase-dead MLCK-GFP

   Plasmid

   #46317

   Depositor

   Anne Bresnick

   Article

   Dulyaninova et al J Cell Sci. 2004 Mar 15;117(Pt 8):1481-9

   Insert

   HeLa long myosin light chain kinase-dead (MYLK Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   E1626K

   Promoter

   CMV

   Available since

   July 18, 2013

   Availability

   Academic Institutions and Nonprofits only

5. FUW-TetO-Esrrb

   Plasmid

   #40798

   Depositor

   Rudolf Jaenisch

   Article

   Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

   Insert

   Esrrb (Esrrb Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal CMV + TetO

   Available since

   Oct. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

6. Designed VEGF-binding scFv G6des13

   Plasmid

   #110213

   Purpose

   Yeast Surface Display of G6des13

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   G6des13
   

   Use

   Tags

   cmyc tag

   Expression

   Yeast

   Mutation

   L A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95F

   Promoter

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUW-TetO-Ctcf

   Plasmid

   #40801

   Depositor

   Rudolf Jaenisch

   Article

   Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

   Insert

   Ctcf (Ctcf Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Minimal CMV + TetO

   Available since

   Oct. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. Designed VEGF-binding scFv G6des1

   Plasmid

   #110212

   Purpose

   Yeast Surface Display of G6des1

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   G6des1
   

   Use

   Tags

   cmyc tag

   Expression

   Yeast

   Mutation

   L A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…

   Promoter

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNH605-PACE-YFP

   Plasmid

   #172002

   Purpose

   Fluorescent reporter for Rpn4 activity

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   4xPACE-YFP
   

   Use

   Vector for genomic integration into the yeast gen…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   CYC1 core promotor with 4 tandem repeats of Rpn4 …

   Available since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCCLc-MND-B2705-SABR-Backbone

    Plasmid

    #119051

    Purpose

    3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site.

    Depositor

    David Baltimore

    Article

    Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2+-HypocratesCS

    Plasmid

    #161960

    Purpose

    Mammalian expression of non-targeted inactivated control version of genetically encoded biosensor for (pseudo)hypohalous acids and their derivatives

    Depositor

    Dmitry Bilan

    Article

    Kostyuk et al Nat Commun. 2022 Jan 10;13(1):171. doi:

    Insert

    Hypocrates
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Cysteine 355 to Serine

    Promoter

    CMV, SP6

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid

    #102887

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid

    #102888

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-flex-GFP-shRNA-scramble

    Plasmid

    #182503

    Purpose

    Expresses scramble shRNA for control expts. Flexed cassette driven by pan neuronal gene promoter

    Depositor

    William Wisden

    Article

    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

    Insert

    pan neuronal promoter driving flexed GFP-shRNA scramble from mir 30 cassette
    

    Use

    AAV, Cre/Lox, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCCLc-MND-B2705-KK10-SABR

    Plasmid

    #119053

    Purpose

    3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the HIV(gag) KK10 epitope "KRWIILGLNK" cloned via BsmBI

    Depositor

    David Baltimore

    Article

    Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

    Insert

    B2705-KK10-SABR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MND

    Available since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMG81-MBP-t-M.MpeI-N374K-His

    Plasmid

    #197985

    Purpose

    Expresses CpG Carboxymethyltransferase (M.MpeI N374K) in bacteria. The gene also encodes an N-terminal (TEV cleavable) MBP tag as well as C-terminal His tag.

    Depositor

    Rahul Kohli

    Article

    Wang et al Nat Chem Biol. 2023 Jun 15. doi: 10.1038

    Insert

    MBP-t-M.MpeI-N374K-His
    

    Use

    Tags

    His and MBP

    Expression

    Bacterial

    Mutation

    M.MpeI-N374K

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMIR-21 Reporter

    Plasmid

    #71870

    Purpose

    Mammalian expression vector for the analysis of miR-21 activity

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    Reverse complementary sequence of miR-21
    

    Use

    Tags

    firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNH605-CCAAT-YFP

    Plasmid

    #172132

    Purpose

    Fluorescent reporter for HAP complex activity

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Insert

    4xCCAAT-YFP
    

    Use

    Vector for genomic integration into the yeast gen…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CYC1 core promotor with 4 tandem repeats of the H…

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FH1-MGT#1-mVenus

    Plasmid

    #164094

    Purpose

    Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

    Depositor

    Gaetano Gargiulo

    Article

    Schmitt et al Cancer Discov. 2021 Mar;11(3):754-777. d

    Insert

    MGT#1-mVenus
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX5X.1 PINK1 KD

    Plasmid

    #13322

    Depositor

    Mark Cookson

    Article

    Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

    Insert

    PINK1 kinase domain (PINK1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Amino acids 112-496; Kinase dead: K219D/D362A/D3…

    Promoter

    Available since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only