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We narrowed to 3,609 results for: golden gate

Showing: 741 - 760 of 3609 results

  1. pUCC001

    Plasmid
    #124451
    Purpose
    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
    Depositor
    John Morrissey
    Article
    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97
    Insert
    HH-BsaI
    Use
    Tags
    Expression
    Yeast
    Mutation
    gRNA expression cassette from pUDP002 modified to…
    Promoter
    TDH3 promoter from S.cerevisiae (already present …
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124451-plasmid-map-sequence-id-257763

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  2. MSGV Mouse Acceptor

    Plasmid
    #128176
    Purpose
    A TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR ref Biotechniques. 2015 Mar 1;58(3):135-9
    Depositor
    David Ott
    Article
    Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    MSCV
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128176-plasmid-map-sequence-id-252503

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  3. pCASB

    Plasmid
    #190175
    Purpose
    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence
    Depositor
    Jessica Tyler
    Article
    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:
    Insert
    sgRNA cloning site
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Added 5'-agagacc-3' upstream and 5'…
    Promoter
    Available since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    190175-plasmid-map-sequence-id-372326

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  4. pKEK3395

    Plasmid
    #234079
    Purpose
    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.
    Depositor
    Karl Klose
    Article
    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000
    Insert
    YukonOFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pJ23100-BsaI
    Available since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234079-plasmid-map-sequence-id-464930

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  8. pLPO3

    Plasmid
    #209977
    Purpose
    Contains Level 0 Part: replication origin (pAMBeta1) for the construction of Level 1 plasmids
    Depositor
    Thorsten Mascher
    Article
    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2
    Insert
    replication origin (pAMBeta1)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    209977-plasmid-map-sequence-id-419379

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  13. pGTRm

    Plasmid
    #127197
    Purpose
    pGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequences
    Depositor
    Jordan Green
    Article
    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6
    Insert
    tRNA-gRNA PCR template
    Use
    Template plasmid for generation of golden gate pc…
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    127197-plasmid-map-sequence-id-248423

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Showing: 741 - 760 of 3609 results