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Plasmid#217328PurposePurification of rat DPP9 fusion with C-terminal FLAG tag from insect cellsDepositorInsertDPP9
UseTagsFLAG and His-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-rDPP9(S729A)
Plasmid#217327PurposePurification of catalytically-dead rat DPP9 from insect cellsDepositorInsertDPP9
UseTagsHis-TEVExpressionInsectMutationS729APromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-BN-rNLRP1-FLAG
Plasmid#217323PurposePurification of Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual-GST-TEV-SINTBAD-mCherry
Plasmid#208874PurposeFor expression in insect cells of SINTBAD-mCherryDepositorInsertSINTBAD (TBKBP1 Human)
UseTagsGST-TEVExpressionInsectMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)
Plasmid#198072PurposeUsed for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)DepositorInsertTBK1 (TBK1 Human)
UseTagsGST-TEV-EGFPExpressionInsectMutationTBK1ΔCTD (res 1-666)PromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual PNG172-STII PLU-HIS
Plasmid#112992PurposeBaculovirus expression of Drosophila png172-Flag and plu-HisDepositorUseTagsFlag and HisExpressionInsectMutationpng172 mutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual PNG-STII PLU-HIS
Plasmid#112993PurposeBaculovirus expression of Drosophila png-Flag and plu-HisDepositorUseTagsFlag and HisExpressionInsectMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZC09 (pFA6a-TEV-6xGly-PrtA-His3MX)
Plasmid#44073DepositorTypeEmpty backboneUsePcr-based yeast c-terminal taggingTags6xGly linker, ADH1 transcription terminator, His3…ExpressionMutationPromoterAvailable sinceApril 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHT (His10-4xFRB-SNAP-Lck G2A)
Plasmid#177887PurposeBaculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2ADepositorInsertLck (LCK Human)
UseTags10xHis-TEV-4xFRB-SNAP tagExpressionInsectMutationG2APromoterPolyhedrinAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 human full-length CENP-E-mNeon-6xHis
Plasmid#180484PurposepFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cellsDepositorInsertCENP-E (CENPE human, Synthetic, Human)
UseTagsmNeon-6xHisExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual His6 TEV LIC cloning vector (5B)
Plasmid#30122DepositorTypeEmpty backboneUseTagsHis6-TEVExpressionInsectMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1-PGAL1-GFP (F64L, S65T, V163A)
Plasmid#53206PurposeN-terminal chromosomal tagging with stable GFP under control of GAL promoterDepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A) and TRP1ExpressionMutationPromoterGAL1 promoterAvailable sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a SVEM hphMX6 lys1-I-SceI cleavage site
Plasmid#67648PurposeI-SceI cleavage site at lys1-hphMX6DepositorInsertsUseTo test i-scei cleavage efficiencyTagsExpressionMutationPromoterAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GFP (F64L, S65T, V163A)
Plasmid#53205PurposeN-terminal chromosomal tagging with stable GFP under control of GAL promoterDepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A) and kanMX6ExpressionMutationPromoterGal1 promoterAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)
Plasmid#79031PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(African green monkey pygerythrus)
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterpolyhedrinAvailable sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-HIS3MX6-PGAL1-GFP (F64L, S65T, V163A)
Plasmid#53207PurposeN-terminal chromosomal tagging with stable GFP under control of GAL promoterDepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsGFP (F64L, S65T, V163A) and HIS3MX6ExpressionMutationPromoterGAL1 promoterAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D)
Plasmid#79036PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5Cyp (owl monkey) (K283D, Q287D)
UseTagsOneSTrEP-FLAGExpressionInsectMutationChanged Lys 283 to Asp and Gln 287 to AspPromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-rDPP9(S729A)-FLAG
Plasmid#217329PurposePurification of catalytically-dead rat DPP9 fusion with C-terminal FLAG tagfrom insect cellsDepositorInsertDPP9
UseTagsFLAG and His-TEVExpressionInsectMutationS729APromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-BN-rNLRP1(N61K)-FLAG
Plasmid#217325PurposePurification of Brown Norway rat NLRP1 (N61K mutation) fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationN61KPromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB His-TEV-BN-rNLRP1(S969A)-FLAG
Plasmid#217324PurposePurification of autocleavage-deficient Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cellsDepositorInsertNLRP1
UseTagsFLAG and His-TEVExpressionInsectMutationS969APromoterpolyhedrinAvailable sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only