We narrowed to 7,351 results for: ski

1. pU6-TREX1 g2 Cas9-T2A-mCherry

   Plasmid

   #164251

   Purpose

   Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   sgTREX1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3-lyn10-mCherry- LOVPLD-P (LOVPLD)

   Plasmid

   #219555

   Purpose

   PM-targeted LOVPLD

   Depositor

   Jeremy Baskin

   Article

   Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

   Insert

   lyn10-mCherry-LOVPLD
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSETT4gap

   Plasmid

   #153413

   Purpose

   φC31-integrated plasmid including BsaI-recognition sites and T4-terminators surrounding the expression cassette

   Depositor

   Joern Kalinowski

   Article

   Schaffert et al Microbiol Resour Announc. 2020 Sep 24;9(

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   gapDH promoter from Eggerthella lenta

   Available since

   July 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCellFree_G03 WWP1

   Plasmid

   #67125

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   WWP1 (WWP1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCellFree_G04

   Plasmid

   #67138

   Purpose

   Gateway destination vector for cell-free expression with C-term eGFP 8xHis tag

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

   Type

   Empty backbone

   Use

   Cell-free expression

   Tags

   EGFP and HIS

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pICSL50005

   Plasmid

   #117536

   Purpose

   Level0 Golden Gate part: c-terminal tag, YFP

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   c-term tag YFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   G65S, L68V, A72S, Y203T, L231H

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)

   Plasmid

   #173453

   Purpose

   Contains mCherry-mNeonGreen tandem fluorescent protein timer

   Depositor

   Anton Khmelinskii

   Article

   Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

   Insert

   mCherry-mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   no promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Endosome-targeted optodPLD

   Plasmid

   #140055

   Purpose

   Encodes CRY2-mCherry-dPLD-P2A-CIBN-2xFYVE for mammalian expression

   Depositor

   Jeremy Baskin

   Article

   Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Phospholipase D
   

   Use

   Tags

   2xFYVE, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Mutation

   H170A (catalytic dead)

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCellFree_G02

   Plasmid

   #67136

   Purpose

   Gateway destination vector for cell-free expression with C-term 8xHis tag

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

   Type

   Empty backbone

   Use

   Cell-free expression

   Tags

   His

   Expression

   Mutation

   Promoter

   Available since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCellFree_G03 POLD1

    Plasmid

    #67128

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    POLD1 (POLD1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pU6-TREX1 g1 Cas9-T2A-mCherry

    Plasmid

    #164250

    Purpose

    Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgTREX1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJEP230-Lenti-TRE3G-4xMyc-GluN2B(E1479Q)-WPRE-pA

    Plasmid

    #164795

    Purpose

    pLenti vector backbone designed to express 4XMyc Tagged-GluN2B(E1479Q) from a TRE3G promoter.

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    GluN2B
    

    Use

    Lentiviral

    Tags

    4xMyc

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEPOR0SP0020

    Plasmid

    #117531

    Purpose

    Level0 Golden Gate part: CDS, SaCas9

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SaCas9 (with stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCellFree_G03 TFAP2B

    Plasmid

    #67092

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TFAP2B (TFAP2B Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCellFree_G03 RAD54B

    Plasmid

    #67124

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    RAD54B (RAD54B Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pIRES_iSeroSnFR_cytosolic

    Plasmid

    #214132

    Purpose

    Fluorescent biosensor for intracellular serotonin (5-HT)

    Depositor

    Steffen Sinning

    Article

    Bukowski et al Research Square PREPRINT. 18 August 2023

    Insert

    iSeroSnFR_cytosolic
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC-PTP-NEO

    Plasmid

    #51710

    Purpose

    contains a C-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

    Depositor

    Arthur Gunzl

    Article

    Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

    Type

    Empty backbone

    Use

    T. brucei genome integration vectors

    Tags

    PTP (ProtC-TEV-ProtA)

    Expression

    Mutation

    Promoter

    Available since

    March 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCHNFI-B2

    Plasmid

    #31405

    Depositor

    Richard Gronostajski

    Article

    Chaudhry et al J Biol Chem. 1998 Jul 17. 273(29):18538-

    Insert

    Nuclear Factor IB isoform 2 (Nfib Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Not the same as reference seq. See sequence for m…

    Promoter

    Available since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pN-PURO-PTP

    Plasmid

    #51711

    Purpose

    contains an N-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

    Depositor

    Arthur Gunzl

    Article

    Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

    Type

    Empty backbone

    Use

    T. brucei genome integration vectors

    Tags

    PTP (ProtC-TEV-ProtA)

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMLC-BB1

    Plasmid

    #166922

    Purpose

    Backbone for assembly of multi-level gene expression controllers

    Depositor

    Thomas Gorochowski

    Article

    Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

    Insert

    CyOFP - Orange fluorescent protein - flanked by BsaI sites
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23100 constitutive

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge