We narrowed to 1,232 results for: sumo

1. USPL1cat-pETM30

   Plasmid

   #85768

   Purpose

   bacterial expression of USPL1 catalytic domain (aa 212-514) as His-GST-TEVsite fusion

   Depositor

   Frauke Melchior

   Article

   Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

   Insert

   USPL1, catalytic domain (aa 212-514) (USPL1 Human)
   

   Use

   Tags

   GST and His

   Expression

   Bacterial

   Mutation

   catalytic domain (aa 212-514)

   Promoter

   T7-lac

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. hGATA-1 K137R

   Plasmid

   #118353

   Purpose

   This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hGATA-1 (GATA1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K137R

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. hHSF1 K298R

   Plasmid

   #118362

   Purpose

   This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K298R

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. USPL1cat-pEYFP_C1

   Plasmid

   #85766

   Purpose

   mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion

   Depositor

   Frauke Melchior

   Article

   Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

   Insert

   USPL1, catalytic domain (aa 212-514) (USPL1 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   catalytic domain (aa 212-514)

   Promoter

   CMV

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pInducer10-UBC9-sh4

   Plasmid

   #168988

   Purpose

   Expresses RFP cDNA and UBC9 shRNA 4 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Ubiquitin Conjugating Enzyme 9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. hHSF1 S303A

   Plasmid

   #118363

   Purpose

   This plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   S303A

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. NP-11-63

   Plasmid

   #234358

   Purpose

   Expressing and purifying R.rubrum rubisco using a his-sumo tag

   Depositor

   David Savage

   Article

   Prywes et al Nature. 2025 Jan 22. doi: 10.1038/s41586

   Insert

   R.rubrum rubisco
   

   Use

   Synthetic Biology

   Tags

   N-term SUMO tag

   Expression

   Mutation

   Promoter

   T7

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EGFP-NIP45 delta-SLD1

   Plasmid

   #210264

   Purpose

   Inducible expression of N-terminally EGFP-tagged NIP45 delta-SLD1. Use with Flp-In T-Rex system.

   Depositor

   Niels Mailand

   Article

   Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

   Insert

   NIP45 delta-SLD1 (delta aa 260-335) (NFATC2IP Synthetic)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   delta-SLD1

   Promoter

   CMV/TetO2

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EGFP-NIP45 D394R

   Plasmid

   #210265

   Purpose

   Inducible expression of N-terminally EGFP-tagged NIP45 D394R resistant to siRNA. Use with Flp-In T-Rex system.

   Depositor

   Niels Mailand

   Article

   Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

   Insert

   NIP45 D394R siRES (NFATC2IP Synthetic)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D394R, siRES

   Promoter

   CMV/TetO2

   Available since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EGFP-NIP45 delta-N1

    Plasmid

    #210268

    Purpose

    Inducible expression of N-terminally EGFP-tagged NIP45 delta-N1 resistant to siRNA. Use with Flp-In T-Rex system.

    Depositor

    Niels Mailand

    Article

    Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

    Insert

    NIP45 delta-N1 (aa 208-419) siRES (NFATC2IP Synthetic)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta-N1, siRES

    Promoter

    CMV/TetO2

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EGFP-NIP45 delta-N2

    Plasmid

    #210269

    Purpose

    Inducible expression of N-terminally EGFP-tagged NIP45 delta-N2 resistant to siRNA. Use with Flp-In T-Rex system.

    Depositor

    Niels Mailand

    Article

    Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

    Insert

    NIP45 delta-N2 (aa 261-419) siRES (NFATC2IP Synthetic)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta-N2, siRES

    Promoter

    CMV/TetO2

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mCherry-NIP45 D394R

    Plasmid

    #210267

    Purpose

    Inducible expression of N-terminally mCherry-tagged NIP45 D394R resistant to siRNA. Use with Flp-In T-Rex system.

    Depositor

    Niels Mailand

    Article

    Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

    Insert

    NIP45 D394R siRES (NFATC2IP Synthetic)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D394R, siRES

    Promoter

    CMV/TetO2

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mCherry-NIP45 wt

    Plasmid

    #210266

    Purpose

    Inducible expression of N-terminally mCherry-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

    Depositor

    Niels Mailand

    Article

    Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

    Insert

    NIP45 wt siRES (NFATC2IP Synthetic)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    siRES

    Promoter

    CMV/TetO2

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EGFP-NIP45 delta-SLD2

    Plasmid

    #210263

    Purpose

    Inducible expression of N-terminally EGFP-tagged NIP45 delta-SLD2. Use with Flp-In T-Rex system.

    Depositor

    Niels Mailand

    Article

    Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

    Insert

    NIP45 delta-SLD2 (aa 1-343) (NFATC2IP Synthetic)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    delta-SLD2

    Promoter

    CMV/TetO2

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. EGFP-NIP45 wt

    Plasmid

    #210262

    Purpose

    Inducible expression of N-terminally EGFP-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.

    Depositor

    Niels Mailand

    Article

    Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

    Insert

    NIP45 wt siRES (NFATC2IP Synthetic)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    siRES

    Promoter

    CMV/TetO2

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCA528 CHMP4C, residues 156-233, no Cys

    Plasmid

    #180642

    Purpose

    Bacterial expression for CHMP4C C-terminal tail, residues 156-233. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-14.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP4C (CHMP4C Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    residues 316-366

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCA528 IST1, residues 315-360, no Cys

    Plasmid

    #180643

    Purpose

    Bacterial expression of IST1 C-terminal tail, residues 315-360. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-99.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    IST1 (IST1 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    residues 315-360

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCA528 KATNA1 MIT residues 1-79

    Plasmid

    #180614

    Purpose

    Bacterial expression for KATNA1 MIT domains, residues 1-79. Has N-terminal HIS-SUMO tag. Internal ID: WISP19-20.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-79

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCA528 KATNA1 MIT residues 1-79, V55D

    Plasmid

    #180615

    Purpose

    Bacterial expression for KATNA1 MIT domains, residues 1-79. Has V55D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-23.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-79, V55D mutation

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCA528 KATNAL1 MIT residues 1-87

    Plasmid

    #180618

    Purpose

    Bacterial expression for KATNAL1 MIT domains, residues 1-87. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-54.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNAL1 (KATNAL1 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-87

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge