We narrowed to 10,431 results for: plasmids 101

1. loxP-G418-LoxP-TurboID-Rab11 HR

   Plasmid

   #230026

   Purpose

   Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells

   Depositor

   Francesca Bottanelli

   Article

   Adarska et al STAR Protoc. 2025 Aug 4;6(3):103949. doi

   Insert

   genomic sequence Rab11 locus (RAB11A Human)
   

   Use

   CRISPR

   Tags

   Turbo-ID, V5

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-tetO-NGN2

   Plasmid

   #197090

   Purpose

   PiggyBac plasmid with tet-inducible expression of transcription factors for cortical neuron differentiation

   Depositor

   Brian Wainger

   Article

   Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

   Insert

   NGN2 (NEUROG2 Human)
   

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPBpuro-NRG1-ScNeo

   Plasmid

   #209906

   Purpose

   To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   NRG1-ScNeo (NRG1 Human)
   

   Tags

   mNeonGreen and mScarlet

   Expression

   Mammalian

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. LV-Sox10MCS5-GFP-EF1a-mCherry

   Plasmid

   #115782

   Purpose

   Sox10-MCS5-GFP and a constitutive EF1α-mCherry reporter plasmid. Construct generates constitutive red fluorescence and basal promoter cfos conjugated SOXMCS5 enhancer driven GFP expression in a cell.

   Depositor

   Fraser Sim

   Article

   Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1

   Inserts

   mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
   EF1alpha promoter driven constitutive mCherry
   

   Use

   Lentiviral

   Tags

   EF1α promoter fused to mCherry and cfos promoter …

   Promoter

   SOX10 Multiple Species Conserved enhancer element…

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLIK-IDH2-FLAG

   Plasmid

   #66806

   Purpose

   Tet-inducible expression of IDH2 in mammalian cells

   Depositor

   Christian Metallo

   Article

   Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

   Insert

   IDH2 (IDH2 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Bxb1-GT_AIO-TetOn_mNgn2 _Down-Tandem

   Plasmid

   #229795

   Purpose

   Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of mouse neurogenin 2

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-mNgn2; rtTA-T2A-mTagBFP2 (Neurog2 Mouse)
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   TRE and CAG

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-tetO-Esrrb

   Plasmid

   #71152

   Purpose

   Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Esrrb

   Depositor

   Hubert Schorle

   Article

   Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

   Insert

   Estrogen related receptor, beta (Esrrb Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSIIpuro-NRG1-ScNeo

   Plasmid

   #209900

   Purpose

   To monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   NRG1-ScNeo (NRG1 Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen and mScarlet

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-DIO {ChETA-mRuby2}on-W3SL

   Plasmid

   #111389

   Purpose

   AAV vector with hSynapsin promoter, Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   ChETA-mRuby2
   W3SL
   

   Use

   AAV

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   W3SL is built from a shortened WPRE, an upstream …

   Promoter

   hSynapsin

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLPC-MYC-hTRF1

    Plasmid

    #64164

    Purpose

    Retroviral vector expressing human TRF1 with N-terminal MYC tag

    Depositor

    Titia de Lange

    Article

    Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

    Insert

    hTRF1 (TERF1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9-HF1-plus

    Plasmid

    #126768

    Purpose

    Expression plasmid for human codon-opt. increased fidelity SpCas9-HF1-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A; Q695A; Q926A; amino acids 1005-1013 replac…

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCKC775

    Plasmid

    #226626

    Purpose

    piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT S. pyogenes, Human)
    EF1a promoter, sfGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    7xTCF-LEF and EF1a

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCKC764

    Plasmid

    #226625

    Purpose

    piggyBAC pCAG-hCas9-T2A-Unbiased3

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Chicken, S. pyogenes, Human)
    EF1a promoter, blasticidin resistance
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV and EF1a

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCKC776

    Plasmid

    #226627

    Purpose

    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT S. pyogenes, Human)
    EF1a promoter, mCherry
    

    Use

    Synthetic Biology

    Tags

    oxygen dependent degron (ODD)

    Expression

    Mammalian

    Promoter

    4xHRE_YB TATA and EF1a

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLL3.7-CAG-hMGT-Ef1a-puro

    Plasmid

    #172395

    Purpose

    Lentiviral expression vector for human direct cardiac reprogramming

    Depositor

    Li Qian

    Article

    Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

    Insert

    Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)
    

    Use

    Lentiviral

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-DIO {mCAR}off-{GCaMP6f}on-WPRE

    Plasmid

    #111393

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON GCaMP6f (ultrasensitive calcium sensor)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    mCAR (Cxadr Mouse)
    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis, Myc, T7, and Xpress

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLL3.7-UBCp-hMGT-Ef1a-puro

    Plasmid

    #172396

    Purpose

    Lentiviral expression vector for human direct cardiac reprogramming

    Depositor

    Li Qian

    Article

    Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

    Insert

    Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)
    

    Use

    Lentiviral

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a_MYC deltaMBII_P2A_Hygro_Barcode

    Plasmid

    #120505

    Purpose

    Barcoded lentiviral vector to express c-MYC deltaMBII in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    c-MYC deltaMBII (MYC Human)
    

    Use

    Lentiviral

    Mutation

    Deletion of MYC Box II domain

    Promoter

    EF1a

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCSIIpuro-BTC-ScNeo

    Plasmid

    #209898

    Purpose

    To monitor the status of Betacellulin, the plasmid encodes a recombinant BTC fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    BTC-ScNeo (BTC Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mScarlet

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-DIO {hCAR}off-{ChETA}on-WPRE

    Plasmid

    #111388

    Purpose

    AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

    Depositor

    Adam Kepecs

    Article

    Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

    Inserts

    hCAR (CXADR Human)
    ChETA
    

    Use

    AAV

    Tags

    HA and Myc

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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