We narrowed to 15,702 results for: grna

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lenti-EGFR-L858R-T790M-dual-epegRNA

Plasmid

#214100

Purpose

Lentiviral vector expressing epegRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two epegRNAs using independent U6 promoters.

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

EGFR L858R epegRNA/EGFR T790M epegRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-RNF2-tevopreQ1-epegRNA+5G>T_EF1a-puroR

Plasmid

#207355

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 engineered prime editing guide (epegRNA) and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

RNF2 + 5G>T tevopreq1-prime editing guide RNA (epegRNA)

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer

Plasmid

#86195

Purpose

U6 based expression of N meningitidis sgRNA

Depositor

Scot Wolfe

Article

Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

Insert

nmCas9 sgRNA cloning cassete

Use

Lentiviral

Promoter

U6

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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LsgRNA-MS2

Plasmid

#235597

Purpose

sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. The cloning site is BsmbI.

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-XRCC1-gRNA res-Neo

Plasmid

#176149

Purpose

XRCC1 with dual PAM resistance to XRCC1 gRNA1 and XRCC1 gRNA2 & a neomycin/G418 resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

X-ray repair cross complementing 1 (XRCC1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Dual PAM resistance to XRCC1 gRNA 1 and XRCC1 gRN…

Promoter

CMV

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJR100

Plasmid

#187240

Purpose

Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-BFP marker expression

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector for Perturb-seq with mU6 promoter

Use

Lentiviral

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pDY2259 hNOLC1 atgRNA Paired Guide 1

Plasmid

#220989

Purpose

hNOLC1 atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

NOLC1 atgRNA (NOLC1 Human)

Use

CRISPR

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDY2260 hNOLC1 atgRNA Paired Guide 2

Plasmid

#220990

Purpose

hNOLC1 atgRNA

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

Insert

NOLC1 atgRNA (NOLC1 Human)

Use

CRISPR

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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HEK4-53bp-tag-insertion-pegRNA

Plasmid

#227673

Purpose

PegRNA for 53 bp insertion

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

SpCas9 sgRNA scaffold (EPHA3 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-KRAS-G13A-tmpknot-epegRNA

Plasmid

#214095

Purpose

Lentiviral vector expressing epegRNA to induce KRAS G13A mutation

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

KRAS G13A epegRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-GBA-N370S-nick sgRNA

Plasmid

#214096

Purpose

Lentiviral vector expressing nicking sgRNA to induce GBA N370S mutation using PE3

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

KRAS G13A nicking sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA-Cre-GpNluc-sgControl

Plasmid

#208383

Purpose

This sgControl, located in the TP53BP1 intron, serves as a control sgRNA for the others. The vector was cloned from Lenti-sgRNA-Cre-GpNLuc.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgControl (TP53BP1 intron) (Trp53bp1 Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

WT

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA-Cre-GpNLuc-sgmcGAS

Plasmid

#208385

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine cGAS gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgcGAS (Cgas Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

WT

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-sgRNA-Cre-GpNLuc-sgmSTING

Plasmid

#208386

Purpose

Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine STING gene.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

sgSTING (Sting1 Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Mutation

WT

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-AAVS1-sgRNA

Plasmid

#194723

Purpose

based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA that target hAAVS1

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1 (AAVS1 Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

Plasmid

#133793

Purpose

U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

CjeCas9 sgRNA entry vector

Expression

Mammalian

Mutation

V2-TGAA linker sgRNA architecture from Kim et al.…

Promoter

U6

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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8xCTS1_WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

Plasmid

#124546

Purpose

Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

Depositor

Tudor Fulga

Article

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Insert

WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro

Expression

Mammalian

Mutation

WT, WT

Promoter

Minimal ADE

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

Plasmid

#124543

Purpose

Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

Depositor

Tudor Fulga

Article

Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

Insert

deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro

Expression

Mammalian

Mutation

d11-41 C55G, d11-41

Promoter

Minimal ADE

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-GFP sgRNA Target 3- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

Plasmid

#210739

Purpose

Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 3

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Inserts

Sp Cas9 (cas9 S pyogenes)
Sp sgRNA GFP Target 3

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Promoter

H1 and eCMV

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-GFP sgRNA Target 1- eCMV- S.pyoegenes-3XFLAG-SV40NLS-ITR2

Plasmid

#210737

Purpose

Coding for Sp Cas9 alongside Sp sgRNA targeting GFP Target 1

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Inserts

Sp Cas9 (cas9 S pyogenes)
Sp sgRNA GFP Target 1

Use

CRISPR

Tags

3xFLAG, SV40 NLS, PolyA signal

Expression

Mammalian

Promoter

H1 and eCMV

Available Since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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