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We narrowed to 9,921 results for: pET

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Showing: 7581 - 7600 of 9921 results

  1. Kif1A-EGFP-SNAP

    Plasmid
    #229851
    Purpose
    Kif1A (aa1-351)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif1A was used to link an oligo to the motor using the SNAP tag for single molecule experiments
    Depositor
    William Hancock
    Insert
    Kif1A-EGFP-SNAP (Kif1a Rat)
    Tags
    6xHis, EGFP, and SNAP-tag
    Expression
    Bacterial
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229851-plasmid-map-sequence-id-460711

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  4. 6XKC

    Plasmid
    #162271
    Purpose
    Bacterial Expression
    Depositor
    Cheryl Arrowsmith
    Insert
    FEM1C (FEM1C Human)
    Tags
    6xHis, GST and Thrombin
    Expression
    Bacterial
    Promoter
    GST-F:tgaagctatcccacaaattg
    Available since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162271-plasmid-map-sequence-id-319836

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  6. ITPAA

    Plasmid
    #42345
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ITPAA (ITPA Human)
    Tags
    His6-Thrombin
    Expression
    Bacterial
    Mutation
    See Comments
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. PP-MAPK1 (3OZ6)

    Plasmid
    #28164
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    mitogen-activated protein kinase 1 (cgd2_1960 Cryptosporidium parvum)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains residues 4-390
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. ASMTL

    Plasmid
    #25156
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ASMTL_02 (ASMTL Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains Maf domain
    Available since
    Feb. 22, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. ARL10B:L9-K182

    Plasmid
    #25523
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ARL10B:L9-K182 (ARL8A Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids L9-K182
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. PP-HSP90 (3HJC)

    Plasmid
    #25271
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    LmjF33.0312:H265-V690
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids H265-V690
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. SRS-MTBD(89/82)-ABux

    Plasmid
    #22384
    Depositor
    Ron Vale
    Insert
    Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
    Tags
    His and myc
    Expression
    Bacterial
    Available since
    Nov. 24, 2009
    Availability
    Academic Institutions and Nonprofits only
    22384-plasmid-map-sequence-id-10292

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  12. SRS-MTBD(19/19), dimer, no myc, ux

    Plasmid
    #22388
    Depositor
    Ron Vale
    Insert
    Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
    Expression
    Bacterial
    Available since
    Nov. 24, 2009
    Availability
    Academic Institutions and Nonprofits only
    22388-plasmid-map-sequence-id-10295

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  14. pNICBio2_WDR5:2-334

    Plasmid
    #211027
    Purpose
    bacterial expression of human WDR5 fragment A2 to C334. N-terminal 10X His tag with TEV cleavage and C-terminal biotin acceptor peptide tag.
    Depositor
    Cheryl Arrowsmith
    Publication
    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.
    Insert
    WDR5:A2-C334 (WDR5 Human)
    Tags
    MHHHHHHHHHHDLGTENLYFQS and SSKGGYGLNDIFEAQKIEWHE
    Expression
    Bacterial
    Mutation
    wild type
    Promoter
    T7
    Available since
    April 4, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    211027-plasmid-map-sequence-id-426277

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  16. pAGH51

    Plasmid
    #107255
    Purpose
    HU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.
    Depositor
    Erin O'Shea
    Publication
    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111
    Insert
    HU-FRB
    Use
    Synthetic Biology
    Tags
    FRB
    Promoter
    native HU promoter
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107255-plasmid-map-sequence-id-207562

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  17. pKK-mVenus-TEV

    Plasmid
    #105774
    Purpose
    Expression of your protein of interest in fusion with yellow fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).
    Depositor
    Andrzej Dziembowski
    Publication
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    mVenus-TEV
    Expression
    Mammalian
    Available since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105774-plasmid-map-sequence-id-203363

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  18. pfMDH_H303A

    Plasmid
    #67730
    Purpose
    Expresses H303A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Publication
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed histidine 303 to alanine
    Promoter
    T7
    Available since
    Sept. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pfMDH_D230A

    Plasmid
    #67725
    Purpose
    Expresses D230A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Publication
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed aspartate 230 to alanine
    Promoter
    T7
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. pfMDH_N191A

    Plasmid
    #67724
    Purpose
    Expresses N191A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Publication
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed asparagine 191 to alanine
    Promoter
    T7
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 7581 - 7600 of 9921 results