We narrowed to 13,664 results for: Cre
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pDR111_Bgal NLS
Plasmid#188396PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptide and SV40 NLSExpressionBacterialPromoterPhyper spankAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-R536C-WLS-HA-IRES-GFP
Plasmid#178065PurposeExpression of WLS-HA protein (R536C mutant)DepositorAvailable SinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_S.FLAG.VSVg_NGFR
Plasmid#158345PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsS.FLAG.VSVgMutationTruncation of the signaling domainPromoterEF1aAvailable SinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO_S.NWS.VSVg_NGFR
Plasmid#158348PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsS.NWS.VSVgMutationTruncation of the signaling domainPromoterEF1aAvailable SinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HA.NWS.V5_NGFR
Plasmid#158248PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsHA.NWS.V5MutationTruncation of the signaling domainPromoterEF1aAvailable SinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HA.NWS.FLAG_NGFR
Plasmid#158249PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsHA.NWS.FLAGMutationTruncation of the signaling domainPromoterEF1aAvailable SinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO_NWS.FLAG.VSVg_NGFR
Plasmid#158233PurposeProtein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).DepositorInsertPro-Code Tagged human dNGFR (NGFR Human)
UseCRISPR and LentiviralTagsNWS.FLAG.VSVgMutationTruncation of the signaling domainPromoterEF1aAvailable SinceSept. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
BE3-NLS-NLS-P2A-BLAST
Plasmid#135348PurposeExpression of BE3 (Base editor generation three) with dual C-terminal NLS in a single operon encoding P2A blasticidine resistanceDepositorInsertBE3-2XNLS
UseCRISPRExpressionMammalianMutationWTAvailable SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS L30 Ruby3-Rab7A
Plasmid#135651PurposeRab7A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.DepositorAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
D5_Ld_280600
Plasmid#135821PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertmajor surface protease gp63, putative,leishmanolysin, putative
Tagsbiotinylation sequence and His tagExpressionMammalianAvailable SinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-RhoAa
Plasmid#109525PurposeMuscle specific zebrafish expression of mKate2 fused to RhoAaDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
actc1b-mKate2-RhoAa-DN
Plasmid#109526PurposeMuscle specific zebrafish expression of mKate2 fused to dominant negative RhoAaDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
C1ORF43-3xFLAG
Plasmid#130880PurposeExpresses human C1ORF43 with C-terminal 3xFLAG tagDepositorAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
JE301: pMVP (L5-L4) Tir1-P2A-AID
Plasmid#121722PurposepMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.DepositorInsertosTir1-P2A-AID (open)
UseGateway Cloning and Synthetic BiologyTags9x myc (C-term on osTIR1)Available SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
myc-hIFITM3-delta1-21
Plasmid#104365PurposeExpresses human IFITM3-delta1-21 with an N-terminal myc tag in mammalian cells.DepositorInsertIFITM3 (IFITM3 Human)
TagsmycExpressionMammalianMutationdeleted amino acids 1-21PromoterCMVAvailable SinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPTK004-2-pTPI1
Plasmid#84963PurposeType 2, pTPI1, Triose phosphate isomerase 1 promoterDepositorInsertTriose phosphate isomerase 1 promoter
UseSynthetic BiologyExpressionYeastPromoterNonAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
miR190 in pcDNA3.1
Plasmid#78122PurposeMammalian expression of miR190DepositorAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3g.a(L)-Fc-His
Plasmid#72151PurposeExpresses the Sema3G, isoform a protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema4f.b-Fc-His
Plasmid#72159PurposeExpresses the extracellular region of the Sema4F, isoform b protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only
